Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEStigmasterolCat.No.:HY-N0131CASNo.:83-48-7Synonyms:Stigmasterin分?式:C??H??O分?量:412.69作?靶點(diǎn):MMP;EndogenousMetabolite作?通路:MetabolicEnzyme/Protease儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)Acetone:6.67mg/mL(16.16mM;Needultrasonic)H2O:<0.1mg/mL(insoluble)1MNaOH:<1mg/mL(insoluble)Ethanol:<1mg/mL(insoluble)DMSO:<1mg/mL(insolubleorslightlysoluble)DMF:1mg/mL(2.42mM;ultrasonicandwarmingandheatto60°C)MEOH:<1mg/mL(insoluble)MassSolvent1mg5mg10mgConcentration制備儲備液1mM2.4231mL12.1156mL24.2313mL5mM0.4846mL2.4231mL4.8463mL10mM0.2423mL1.2116mL2.4231mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時(shí)，請?jiān)?個(gè)?內(nèi)使?，-20°C儲存時(shí)，請?jiān)?個(gè)?內(nèi)使?。1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEBIOLOGICALACTIVITY?物活性Stigmasterol(Stigmasterin)?種植物性甾醇，不僅具有降低膽醇的活性，并且在風(fēng)濕性疾病的研究中被評價(jià)為抗硬化因?。IC50&TargetHumanEndogenousMetabolite體外研究PreincubationofStigmasteroltoIL-1beta-treatedcellsshowssigni?cantreductionofMMP-3mRNAinhumanandmouse,MMP-3proteininmouse,MMP-13mRNAinmouseandhuman,ADAMTS-4mRNAinhuman,PGE2proteininhumanandmouse.StigmasterolisalsocapableofcounteractingtheIL-1beta-inducedNF-κBpathway[1].PROTOCOLCellAssay[1]Amodelofnewbornmousechondrocytesandhumanosteoarthritis(OA)chondrocytesareusedinprimaryculturestimulatedwithorwithoutIL-1β(10ng/mL),for18h.Cellsarepre-incubatedwithStigmasterol(20mg/mL)for48h[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?JEthnopharmacol.2022Aug2;115586.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].GabayO,etal.Stigmasterol:aphytosterolwithpotentialanti-osteoarthriticproperties.OsteoarthritisCartilage.2010Jan;18(1):106-16.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel: