ICS

65.140

CCS

B47

中華人民共和國(guó)國(guó)家標(biāo)準(zhǔn)

GB/T44349—2024

蜂花粉總多酚的檢測(cè)福林酚試劑

比色法

Detectionoftotalpolyphenolsinbeepollen—Colorimetricmethodusing

Folin-Ciocalteureagent

2024-09-29發(fā)布2025-04-01實(shí)施

國(guó)家市場(chǎng)監(jiān)督管理總局發(fā)布

國(guó)家標(biāo)準(zhǔn)化管理委員會(huì)

GB/T44349—2024

目次

前言

·····································································································

Ⅲ

1

范圍

··································································································

1

2

規(guī)范性引用文件

······················································································

1

3

術(shù)語(yǔ)和定義

···························································································

1

4

原理

··································································································

1

5

試劑和材料

···························································································

1

6

儀器設(shè)備

······························································································

2

7

分析步驟

······························································································

2

7.1

試樣制備

·························································································

2

7.2

試樣前處理

······················································································

2

7.3

空白試驗(yàn)

·························································································

2

7.4

標(biāo)準(zhǔn)曲線的制作

·················································································

2

7.5

試樣測(cè)定

·························································································

2

8

結(jié)果計(jì)算

······························································································

3

9

定量限

································································································

3

10

精密度

·······························································································

3

附錄A（資料性）

福林酚試劑（Folin?Ciocalteureagent）配制方法

································

4

參考文獻(xiàn)

··································································································

5

Ⅰ

GB/T44349—2024

前言

本文件按照GB/T1.1—2020《標(biāo)準(zhǔn)化工作導(dǎo)則第1部分：標(biāo)準(zhǔn)化文件的結(jié)構(gòu)和起草規(guī)則》的規(guī)

定起草。

請(qǐng)注意本文件的某些內(nèi)容可能涉及專利。本文件的發(fā)布機(jī)構(gòu)不承擔(dān)識(shí)別專利的責(zé)任。

本文件由中華全國(guó)供銷(xiāo)合作總社提出。

本文件由全國(guó)蜂產(chǎn)品標(biāo)準(zhǔn)化技術(shù)委員會(huì)（SAC/TC601）歸口。

本文件起草單位：中國(guó)計(jì)量大學(xué)、安徽省王巢食品有限公司、廣東正當(dāng)年生物科技股份有限公司、

中科標(biāo)準(zhǔn)（寧德）科技有限公司、睿科集團(tuán)（廈門(mén)）股份有限公司、深圳職業(yè)技術(shù)大學(xué)、秦皇島海關(guān)技

術(shù)中心、阜陽(yáng)職業(yè)技術(shù)學(xué)院、南京眾譜生物科技有限公司、浙江省農(nóng)業(yè)科學(xué)院、浙江省中醫(yī)藥研究院、

中國(guó)農(nóng)業(yè)科學(xué)院蜜蜂研究所、青海省花寶蜂業(yè)股份合作公司、杭州天廚蜜源保健品有限公司、蘭溪市產(chǎn)

品商品質(zhì)量檢測(cè)研究院、山東世通檢測(cè)評(píng)價(jià)技術(shù)服務(wù)有限公司、陜西國(guó)蜂大健康產(chǎn)業(yè)有限公司、江西汪

氏蜜蜂園有限公司。

本文件主要起草人：李紅亮、胡童霞、岳振峰、吳帆、崔宗巖、徐錦忠、曹聯(lián)飛、韓亞超、

張敬群、王磊、趙光培、蔣先志、陳璇、李強(qiáng)強(qiáng)、董捷、李平、林影、李斌、車(chē)延年、陳云、汪玲。

Ⅲ

GB/T44349—2024

蜂花粉總多酚的檢測(cè)福林酚試劑

比色法

1范圍

本文件描述了測(cè)定蜂花粉中總多酚的福林酚試劑比色方法。

本文件適用于蜂花粉中總多酚含量的測(cè)定。

2規(guī)范性引用文件

下列文件中的內(nèi)容通過(guò)文中的規(guī)范性引用而構(gòu)成本文件必不可少的條款。其中，注日期的引用文

件，僅該日期對(duì)應(yīng)的版本適用于本文件；不注日期的引用文件，其最新版本（包括所有的修改單）適用

于本文件。

GB5009.3—2016食品安全國(guó)家標(biāo)準(zhǔn)食品中水分的測(cè)定

GB/T6682分析實(shí)驗(yàn)室用水規(guī)格和試驗(yàn)方法。

3術(shù)語(yǔ)和定義

下列術(shù)語(yǔ)和定義適用于本文件。

3.1

蜂花粉beepollen

工蜂采集花粉，用唾液和花蜜混合后形成的物質(zhì)。

[來(lái)源：GB/T30359—2021，3.2]

4原理

蜂花粉經(jīng)75%乙醇溶液超聲提取的總多酚物質(zhì)的酚羥基在堿性條件下，與福林酚試劑中磷鎢鉬酸的

六價(jià)鎢離子（W6+）反應(yīng)生成藍(lán)色的五價(jià)鎢離子（W5+），可在765nm波長(zhǎng)處產(chǎn)生最大吸收。在一定濃

度范圍內(nèi)，其吸光度與總多酚類化合物含量成正比。與沒(méi)食子酸標(biāo)準(zhǔn)曲線比較，可定量測(cè)定總多酚化合

物的含量。

5試劑和材料

除非另有規(guī)定，僅使用分析純?cè)噭?/p>

5.1水，GB/T6682，三級(jí)。

5.2無(wú)水乙醇。

5.3無(wú)水碳酸鈉。

5.4福林酚試劑（Folin?Ciocalteureagent）：1mol/L（或1N），以棕色瓶分裝，4℃避光保存。直

接購(gòu)買(mǎi)或配制，配制方法見(jiàn)附錄A。

5.57