Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemELacto-N-fucopentaoseICat.No.:HY-W019806CASNo.:7578-25-8Synonyms:LNFPI分?式:C??H??NO??分?量:853.77作?靶點(diǎn):EndogenousMetabolite;CDK;ReactiveOxygenSpecies;Apoptosis;Enterovirus;Bacterial作?通路:MetabolicEnzyme/Protease;CellCycle/DNADamage;Immunology/Inflammation;NF-κB;Apoptosis;Anti-infection儲存?式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY?物活性Lacto-N-fucopentaoseI(LNFPI)?種?母乳寡糖(HMO)，具有抗病毒和抗活性。Lacto-N-fucopentaoseI通過減少?蛋?VP1來阻斷病毒吸附，增加CDK2、減少cyclinE恢復(fù)細(xì)胞周期S期的阻滯。Lacto-N-fucopentaoseI抑制病毒感染的細(xì)胞凋亡(apoptosis)。Lacto-N-fucopentaoseI也抑制ROS的產(chǎn)?。Lacto-N-fucopentaoseI還可以調(diào)節(jié)腸道群響免疫系統(tǒng)發(fā)育。IC50&TargetHumanEndogenousMetaboliteCDK2/cyclinE體外研究Lacto-N-fucopentaoseI(25-3200μg/mL;48h)exhibitscertaincytotoxicityat3200μg/mLbutnotoxicreactionbelow1600μg/mL[1].Lacto-N-fucopentaoseI(25-1600μg/mL;14-18h)canprotectEV71-infectedRDcellsfromdeath[1].Lacto-N-fucopentaoseI(100-400μg/mL;16h)decreasesmarkedlymRNAlevelsofVP1andROSproductioninEV71-infectedRDcellsat400μg/mL;leadstotherecoveryofEV71-inducedSphasearrestinRDcells[1].Lacto-N-fucopentaoseI(100and200μg/mL;3days)inhibitscellapoptosisinCaenorhabditiselegans;significantlydecreasesthelevelsofEgl-1,Ced-3andCed-4[1].Lacto-N-fucopentaoseIcanreducetheabundanceofSphingobacterium,StenotrophomonasandAchromobacter;canincreasetheabundanceofMicromonospora,Vibrio,Acidibacter,Gaiella,Devosia,Steroidobacter,Variibacter,Dactylosporangium,RB41,Pir4_lineage,Pirellula,Haliangium,Roseiflexus,Pedomicrobium,andBradyrhizobium.CellViabilityAssay[1]1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemECellLine:RDcellsConcentration:25,50,100,200,400,800,1600and3200μg/mLIncubationTime:48hResult:Exhibitedcertaincytotoxicityat3200μg/mLbutnotoxicreactionbelow1600μg/mL.RT-PCR[1]CellLine:RDcells(infectedwithEV71)Concentration:100,200and400μg/mLIncubationTime:16hResult:DecreasedmarkedlymRNAlevelsofVP1onlyat400μg/mL.ApoptosisAnalysis[1]CellLine:RDcells(infectedwithEV71)Concentration:100,200and400μg/mLIncubationTime:16hResult:Decreasedtherateofcellsinearlyapoptosisto10.9%±1.26%at400μg/mL,whiletheuntreatedEV71groupwas27.7%±2.13%.Significantlyinhibitedtheactivityofcaspase-3,caspase-8andcaspase-9.RecoveredthedecreasedmRNAexpressionofPAPR,NF-κBandBcl-2andproperlyregulatedBadandFasintotheirnormallevels.CellCycleAnalysis[1]CellLine:RDcells(infectedwithEV71)Concentration:100,200and400μg/mLIncubationTime:12hResult:RescuedEV71-inducedSphasearrest,whichpromotedthetransitionoftheG1phasetotheSphase.REFERENCES[1].GaoX,etal.FucosylatedoligosaccharideLacto-N-fucopentaoseIamelioratesenterovirus71infectionbyinhibitingapoptosis.FoodChemX.2022Feb4;13:100244.McePdfHeight2/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemECaution:Producthasnotbeenfullyvalidate