參松養(yǎng)心膠囊抗心律失常機(jī)理研究

Studyonthemechanismofantiarrhythmiceffects

ofShensongYangXin

藥學(xué)班程義如ABSTRACT

Cardiacarrhythmiaisakindofcommoncardiovasculardiseasesintheclinicalpractice，whichhavegreateffectsonlifequalityandsafetyofthePatients.AtPresent,antiarrhythrmicdrugshavebeenclassifiedassodiumchannelblockers，β-recepterbloekers，actionPotentialdurationProlongagentsandcalciumchannelblockers.Althoughtheclassificationofantiarrhythmicdrugsaredifferent,modulationtheionchannelsfunctionisthebasicmechanism.摘要心律失常是臨床常見且對(duì)患者的生活質(zhì)量和生命安全影響極大地心血管疾病。目前，抗心律失常西藥主要包括鈉離子通道阻滯劑，β受體阻斷劑、延長(zhǎng)動(dòng)作電位時(shí)程藥和鈣離子通道阻斷劑。盡管藥物分類不同，其作用機(jī)制有差異，歸根到底是通過(guò)對(duì)各種心肌細(xì)胞離子通道功能的調(diào)節(jié)來(lái)起作用的。Comparedtowesternmedicine,someantiarrhythmicChineseherbswithmanycomponentscanmulti一targetmodulatethedisorderofionchannelfunction.Itmightexertantiarrhythmiceffortswithlowerincidenceofadverseeffects.同西藥相比，抗心律失常作用的中藥不僅對(duì)心臟離子通道功能有影響，而且中藥往往是復(fù)合藥物，具有多離子通道靶點(diǎn)的作用，因而毒性低副作用小。ShensongYongxin(SSYX)isoneofthecompoundrecipeofChinesemateriamedicalincluding12ingredients.PreviousstudiesonanimalmodelshowedthatSSYXsignificantlyinhibitedthearrhythmiasicinducedbytoxicchemicalcompoundsorischemia一reperfusioninjury.Smallrandomdouble一blindclinicaltrialsalsosuggestedthatSSYXreducedthenumberofventricularextrabeatsinpatientswithorwithoutstructureheartdisease參松養(yǎng)心膠囊(SSYX)是一種復(fù)方制劑，方中多數(shù)藥物均有較好的抗心律失常作用。動(dòng)物實(shí)驗(yàn)證明，SSYX可以明顯抑制有毒化學(xué)藥物或者局部灌注損傷缺血誘發(fā)的心律失常;隨機(jī)臨床驗(yàn)顯示，SSYX對(duì)治療冠心病心律失常有明顯療效。ObjiectivesTodeterminetheeffectsofShensongYangXin(SSYX)onL一tyPecalciumchannels(ICa，L)，sodiumchannels(INa)，transientoutwardpotassiumcurrent(It)，delayedrectifiercurrent.目的觀察ssyx干粉提取溶液對(duì)單個(gè)心室肌細(xì)胞L型鈣電流(Ica，:)，鈉電流(東a)，瞬時(shí)外向鉀電流(It。)，內(nèi)向整流鉀電流(眾1)，延遲整流鉀電流慢成分(反s)的影響，resultsTheactionpotentialandioncurrentcouldberecordedintheisolatedventricularmyocytesofguineapigsandrats，whichindicatedthemyocyteshadsatisfaetoryelectrophysiologicalproperties.Approximate40一60percenttransfectedHEK293cellstookgreenfluorescence，inwhichIkrcould