Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEML348Cat.No.:HY-100736CASNo.:899713-86-1Synonyms:GNF-Pf-1127分?式:C??H??ClF?N?O?分?量:415.79作?靶點(diǎn):Phospholipase作?通路:MetabolicEnzyme/Protease儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:25mg/mL(60.13mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.4051mL12.0253mL24.0506mL5mM0.4810mL2.4051mL4.8101mL10mM0.2405mL1.2025mL2.4051mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoil1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESolubility:≥2.5mg/mL(6.01mM);ClearsolutionBIOLOGICALACTIVITY?物活性ML348(GNF-Pf-1127)?個(gè)選擇性的和可逆的酰蛋?硫酯酶1(APT1)/溶?磷脂酶1(LYPLA1)抑制劑,IC50值為210nM[1]。IC50&TargetIC50:210nM(LYPLA1)體外研究ML348(5μM;3hours)selectivelyinhibitsLYPLA1inculturedcells[1].CellViabilityAssay[1]CellLine:HEK293Tcells,murineT-cellsConcentration:5μMIncubationTime:3hoursResult:Showpotent,selectiveinhibitionofLYPLA1.體內(nèi)研究ML348(50mg/kg,i.p.,3hours)showspotentandselectiveactivityinvivo,inhibitsLYPLA1enzymesinheart,kidneyandlunginmice[1].AnimalModel:C57BL6malemice(3-4months)[1]Dosage:50mg/kgAdministration:Intraperitonealinjection;3hoursResult:PotentandselectiveinhibitedLYPLA1enzymesinheart,kidneyandlunginvivo.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?AdvMater.2021Dec;33(49):e2103471.?NatBiomedEng.2019Apr;3(4):306-317.?NatCommun.2020Sep21;11(1):4765.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].AdibekianAetal.CharacterizationofaSelective,ReversibleInhibitorofLysophospholipase1(LYPLA1).NationalCenterforBiotechnologyInformation(US);2010-2013Apr08.2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEMcePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly