流式染料及應(yīng)用實(shí)例介紹BD生物科學(xué)孔祥濤Sky_Kong@BDIntroductionBD是由MaxwellW.Becton和FairleighS.Dickinson

于1897年在紐約創(chuàng)立的，總部設(shè)在新澤西州。經(jīng)過一百多年的發(fā)展，BD已成為世界上最大的醫(yī)療技術(shù)及醫(yī)療設(shè)備公司之一，它以領(lǐng)先的技術(shù)、卓越的產(chǎn)品質(zhì)量和誠實(shí)可信的服務(wù)贏得了全球用戶及合作伙伴的廣泛贊譽(yù)。FairleghS.DickinsonMaxwellW.BectonBD生物科學(xué)作為BD的一個業(yè)務(wù)組成，一直致力于為生命科學(xué)研究者和臨床醫(yī)生提供創(chuàng)新的臨床和科研工具。其主要產(chǎn)品包括流式細(xì)胞儀；細(xì)胞圖象分析系統(tǒng)；單克隆抗體和試劑盒；藥物研發(fā)產(chǎn)品及臨床診斷試劑等。1973年世界第一臺商品化的流式細(xì)胞儀FACSIBDIntroductionBDHLA-B27KitStemCellsTCellsResearchBDIntroductionBDIntroduction/cn/BDIntroductionFluorochromesOverviewEx-Max482nm/Em-Max695nm.NotsubjecttophotobeachinglikePerCP.NotassusceptibletofixativeorlightinstabilitycomparedtoAPC-Cy7andPE-Cy7.PerCP-Cy?5.5Ex-Max496nm/Em-Max612nm.PE-CF594isabrighteralternativetoPE-TexasRed?,withimprovedspectralcharacteristics.PE-CF594reagentsexhibitveryconsistentspillovervalueslot-to-lot,makingthemanidealchoiceforthePE-TexasRed?detector(610/20nm).BDHorizon?PE-CF594ImprovedbrightnessoverPE-TexasRedECD,andPE-AlexaFluor610BDHorizon?PE-CF594StainIndexandspillovervaluecomparison.BDHorizon?PE-CF594Ex-Max650nm/Em-Max785nm.APC-H7conjugatesprovidegreaterstabilityinlightandparaformaldehydefixativesandhavelessspilloverintotheAPCchannelthanAPC-Cy7conjugates.APC-Cy7andAPC-H7cannotbeusedsimultaneously.APC-H7BDAPC-H7demonstratedstabilityandreducedspillover.Theexperimentsshownherecomparethestability,asmeasuredby%spillover,ofwholebloodstainedwithBDAPC-H7andAPC-AlexaFluor?750conjugatedantibodies.APC-H7BrilliantViolet?421BrilliantViolet?421是一種紫色激光激發(fā)的染料，比其他紫色激光激發(fā)的染料更加明亮。并且，BrilliantViolet比藍(lán)色和紅色激光激發(fā)的染料也更明亮，它是BD提供的最明亮的染料。因此，它特別適合多色應(yīng)用，可以更好地弱表達(dá)細(xì)胞群BrilliantViolet421平均比PacificBlue?明亮10倍，比PE明亮2-3倍BrilliantViolet?421BrilliantViolet?421應(yīng)用BrilliantViolet421開展Treg研究BDHorizon?V450Ex-Max404nm/Em-Max448nm.SpectralpropertiessimilartoPacificBlue?.ComparisonoffluorescenceintensitiesofBDHorizonV450andPacificBlue?reagents.BDHorizon?V450StainindexcomparisonbetweenBDHorizonV450andPacificBlue?reagentsacrossseveralantibodyclones.BDHorizon?V500Ex-Max415nm/Em-Max500nm.ImprovedbrightnessoverPacificOrange?andreducedspilloverintotheFITCchannelwhencomparedtoAmCyan.BDHorizonV500isbrighterthanequivalentPacificOrange?conjugates.BDHorizon?V500BDHorizonV500conjugatesofferdramaticallyreducedspilloverintotheadjacentFITCchannelwhencomparedtoAmCyan.BDHorizon?V500ExampledataofBDHorizonV500usedincombinationwithBDHorizon?V450.BDHorizon?V500BrilliantViolet?605Ex-Max407nm/Em-Max602nm.Tandemfluorochrome,BrilliantViolet421andCy?3.5.ExcitedbythevioletlaseranddetectedwithastandardPE-CF594/TexasRed?filterset(eg,610/20-nmfilter).Verybright,similartoequivalentPEconjugates.Secondorthirdcolor

forthevioletlaser.BrilliantViolet?605BrilliantViolet?605

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