Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEVH032-PEG5-C6-ClCat.No.:HY-112495CASNo.:1799506-06-1Synonyms:HaloPROTAC2分?式:C??H??ClN?O?S分?量:783.41作?靶點(diǎn):E3LigaseLigand-LinkerConjugates作?通路:PROTAC儲(chǔ)存?式:-80°C,protectfromlight,storedundernitrogen溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:270mg/mL(344.65mM;Needultrasonic)Ethanol:100mg/mL(127.65mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM1.2765mL6.3824mL12.7647mL5mM0.2553mL1.2765mL2.5529mL10mM0.1276mL0.6382mL1.2765mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months(protectfromlight,storedundernitrogen)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥5mg/mL(6.38mM);Clearsolution2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESolubility:≥5mg/mL(6.38mM);Clearsolution3.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥5mg/mL(6.38mM);ClearsolutionBIOLOGICALACTIVITY?物活性VH032-PEG5-C6-Cl(HaloPROTAC2)由E3配體與21個(gè)原?相連的復(fù)合分?，連接?的接頭為Halogen團(tuán)。VH032-PEG5-C6-Cl(HaloPROTAC2)包含于VH032的VHL配體和5個(gè)單元PEGlinker。VH032-PEG5-C6-Cl能夠誘導(dǎo)GFP-HaloTag7降解[1]。IC50&TargetVHL體外研究Treatment(24hour)withVH032-PEG5-C6-Clleadstonearly70%degradationofGFP-Halotag7at2.5μM[1].REFERENCES[1].BuckleyDL,etal.HaloPROTACS:UseofSmallMoleculePROTACstoInduceDegradationofHaloTagFusionProteins.ACSChemBiol.2015Aug21;10(8):1831-7.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792;