Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEPRMT5:MEP50PPICat.No.:HY-151576分?式:C??H??N?O?分?量:430.46作?靶點(diǎn):Others作?通路:Others儲(chǔ)存?式:4°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:200mg/mL(464.62mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.3231mL11.6155mL23.2310mL5mM0.4646mL2.3231mL4.6462mL10mM0.2323mL1.1615mL2.3231mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥5mg/mL(11.62mM);ClearsolutionBIOLOGICALACTIVITY1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE?物活性PRMT5:MEP50PPI?種新型的PRMT5:MEP50蛋?-蛋?相互作?(PRMT5:MEP50PPI)抑制劑，其具有抗腫瘤活性和肺癌和前列腺癌細(xì)胞的抗增殖活性。體外研究PRMT5:MEP50PPI(0-10μM;72h)inhibitsthegrowthofprostatecancercells[1].PRMT5:MEP50PPI(250-1000nM;72h)decreasestheexpressionofglobalarginine3residueofhistoneH4(H4R3me2s)levelinprostatecancercells[1].PRMT5:MEP50PPI(500nM;72h)de-repressesesthePRMT5:MEP50-regulatedIVLgeneofprostatecancercells[1].PRMT5:MEP50PPItreatmentinhibitsmultiplePRMT5-regulatedpathwayscriticaltothesurvivalandproliferationoflungandprostatecancercells[1].CellViabilityAssay[1]CellLine:LNCaPcellsConcentration:0-10μMIncubationTime:72hResult:ShowedIC50valueof430.2nM.WesternBlotAnalysis[1]CellLine:LNCaPcellsConcentration:250,500and1000nMIncubationTime:72hResult:Observedglobalarginine3residueofhistoneH4(H4R3me2s)decreasedby65%.RT-PCR[1]CellLine:LNCaPcellsConcentration:500nMIncubationTime:72hResult:De-repressesedthePRMT5:MEP50-regulatedIVLgenewithoutsignificantlyalteringtheexpressionofthePRMT5:pICln-regulatedARgene.REFERENCES[1].AsberryAM,etal.DiscoveryandBiologicalCharacterizationofPRMT5:MEP50Protein-ProteinInteractionInhibitors.JMedChem.2022Oct7.McePdfHeight2/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemECaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forrese