第1題Mostofthetoolenzymesusedingeneticengineeringareisolatedandpurifiedfrommicroorganisms.第2題DNAdoublehelixstructurewaselucidatedbyWatsonandCrickin1953.第3題Theworld'sfirsttransgenicanimal,transgenicmouse,wasfirstproducedbymicroinjectionin1982.第4題

Excisionrepairincludesbaserepairand()AdirectrepairBnucleotideexcisionRepairCmismatchrepairDrecombinationrepair第5題Whatarethemainstepsintranslation()Ainitiation、extension、terminationBinitiation、folding、extension、terminationCfolding、extension、terminationDinitiation、folding、termination第1題DNAhelicaseisnotrequiredduringinvivoreplication.第2題AMVisacommonlyusedreversetranscriptase,andithasno3'→5'exonucleaseactivity.第3題Phi29DNApolymerasehascalibrationactivity,andthereactiontemperatureis30°C,whichdoesnotrequireaPCRmachine.第4題ThemostwidelyusedpolymeraseinthePCRprocessis().AklenowfragmentBT4DNApolymeraseCTaqDNApolymeraseDVentDNApolymerase第5題1.

ThebasicstepofPCRreactionis().Adenaturation-extension-annealingBannealing-extension-denaturationCextension-annealing-denaturationDdenaturation-annealing-extensionChapter3Homework第1題Theessenceofgenecloningistoamplifyandexpressthetargetgeneunderspecificconditions.第2題Receptorbacteriawithoutantibioticresistancegenescannotgrowinmediacontainingantibiotics.第3題Oneofthebasicconditionsthatplasmidvectorsmusthaveisasmallmolecularweightandahighcopynumber.第4題InpBR322plasmid,prepresentsthenameoftheresearcherwhoconstructedtheplasmid.第5題Single-strandedDNAphagecanproducealargenumberofsingle-strandedmoleculescontainingexogenousDNAinserts,whichisconvenientforbeingasprobeorsequencing.Chapter4Homework第1題Intheselectionofreceptorcells,thegeneticstabilityoftheselectedreceptorcellsshouldbehigh,anditshouldbeeasytoexpandthecultivationorfermentationgrowth.第2題Yeastartificialchromosomescanonlybeamplifiedinyeastcells.第3題TransformationreferstotheprocessofintroducingrecombinantλphagemoleculesDNAdirectlyintoreceptorcells.第4題FrederickSangerwontheNobelPrizeinChemistryin1958.Hesequencedtheaminoacidofinsulin.第5題TransfectionreferstotheintroductionofforeignDNAto(

)Aeukaryoticcells(exceptyeast)BbacteriaCmammaliancellsDyeastcellsChapter5Homework第1題Yeasthasnosecretoryfunction,soitcannotmakesomeexogenousproteinssecretetoextracellular.第2題

ThestructureoftheE.coliexpressionsystemisthepromoterandregulatorygenes,ribosomebindingsite,terminatorand().AreplicatingforkBRepliconCattenuatorDduplicatefield第3題

In1979,()wasexpressedinE.coliforthefirsttime.AhumaninsulingeneBgrowthhormonegeneChumaninterferongeneDhumanCD252gene第4題Themostcommonlyusedprokaryoticexpressionsystemis().AinsectexpressionsystemByeastexpressionsystemCe.coliexpressionsystemDexpressionsystemoffilamentousfungi第5題ThehepatitisBvaccineweusenowisproducedby().AyeastexpressionsystemBexpressionsystemoffilamentousfungiCinsectexpressionsystemDmammaliancellexpressionsystemChapter6Homework第1題Thetraditionalmethodofcysteineextractionisextractingitfromhumanoranimal’shair,feathers,bristlesorhorns.第2題HumanbodycannotsynthesizeVitaminCbecausethebodylacksanenzymewhichisneededattheendofthevitaminCbiosynthesispathway,sowemustuptakeVitaminCfromfoodsortakesomeadditionalsupplements.第3題Theendospermofgoldenriceisyellowbecauseitcontainscarotenoids.第4題Insecticidesnotonlyeliminatepests,butalsokillmanyotherinsects,thusaffectingthesensitiveecologicalbalance,andgraduallypoisoningotherorganisms,suchasbirdsthatfeedoninsects.第5題

()thefirstcommercializedhepatitisBvaccinewaslaunched.AIn1980BIn1981CIn1982DIn1983Chapter7Homework第1題Geneticengineeringtechnologywillisolateandmodifyforeigngenescarryingcertainbiologicaltraits,thenintroduceandintegratethemintoanotherorganism,sothatorganismsthatcanstablyinheritandexpressnewtraitsofthecorrespondingorganismarecalledtransgenicorganisms.第2題Transgenicplantscanincreasegrainyield,shortenbreedingyears,reducepesticideconsumption,environmentalpollutionandgreenhousegasemissions.Chapter8Homework第1題WhentheDNAisextracted,thefunctionofproteaseKistodegradethemembraneprotein,degradetheproteinboundtotheDNAsothatfreetheDNAfully.第2題

Howmanynucleotidescanencodeanaminoacid?（）A1B2C3D4第3題

Whichofthefollowingenzymescanresisthightemperature?()ATaqDNApolymeraseBT4DNAligaseCRNaseDHindIII第4題

Themainrole