TheThirdrodPathwayResponsesintheInnerLeiTheFirstAffiliatedHospitalofChongqingMedicalUniversityPurpose:TherodentretinanotexhibitapositiveOFF-responseintheelectroretinogram(ERG),whichmakesitdifficulttoevaluateitsOFF-pathwayfunctionsinWestudiedtherod-drivenOFFresponsesbyusingadark-adapted10-HzflickerERGprocedureinmouse.MaterialsandMethods:ConventionalERGsand10-Hzdark-adaptedflickerERGswereobtainedinwild-typemice(C57BL/6),inmicewithpurerod(cpfl1)orpurecone(rho-/-)function,andinnob1micewhichaselectiveON-pathwaydefect.ToisolatetheresponsefromONorOFFpathway,glutamateogs2-amino-4-phosphobutyricacid(APB,anONpathwayblocker)andcis-2,3-piperidine-dicarboxylicacid(PDA,anOFFpathwayblocker),wereinjectedResults:Theamplitude-intensityprofileofthedark-adaptedflickerERGinthewild-typemiceexhibitstwopeaksatmiddleandhighlightintensities.Thetwopeaksrepresentrod-andcone-drivenresponsesrespectively.InAPB-treatedC57BL/6miceandinmice,thedark-adaptedERGb-waveswereabsent.However,bothrod-andcone-drivenOFFpathwayresponseswereevidentwithflickerERGrecording.Atmiddlelightintensitiesthatactivateonlysystem,theflickerERGresponsesinsaline-injectednob1miceweresimilartothoseinAPB-injectedcpfl1miceandwild-typemice.TheseresponsesaresensitivetoPDA.Theamplitudesoftheserod-drivenOFFpathwayresponseswereapproximay20%ofthetotalrod-drivenflickerERGresponses.Conclusion:Wedemonstratethattherod-OFFbipolarcellpathwayisfunctionalintheouterretina.Thedark-adaptedflickerERGispracticalfortheevaluationofrod-andcone-drivenresponses,andtheresidualOFFpathwaysignalsinsubjectswithONpathway鄭州市眼科醫(yī)院可作為眼生理整合檢查研究的標(biāo)桿。方法：分析視網(wǎng)膜電圖與視力、視野、瞳光反射等檢查的整合關(guān)系。首先，對(duì)ERG與視網(wǎng)膜各級(jí)神經(jīng)元光反應(yīng)的對(duì)比分析，推斷視網(wǎng)膜最有臨床意義的b的。再通過對(duì)自我驗(yàn)證的病理負(fù)ERG錄眼病及其病因分析，驗(yàn)證視網(wǎng)膜電圖在視網(wǎng)膜的細(xì)胞，與視力、視野神經(jīng)通路異同。探索瞳光反射比ERG敏感范圍大的解剖基礎(chǔ)。OCT掃描結(jié)果也提供了相關(guān)眼病雙極細(xì)胞病變形態(tài)。結(jié)果：我們發(fā)現(xiàn)ERGb僅反映全部視網(wǎng)膜雙極細(xì)胞形覺的部分通電反應(yīng)，因?yàn)橹行囊暳椭苓呉曇翱梢哉５那闆r下記錄到病理負(fù)ERG。ERGb神經(jīng)節(jié)細(xì)胞包括ipRGCs，因此ERG也不反映這些時(shí)間通路。ERG能反映分級(jí)電位（ON/OFF應(yīng)）在視網(wǎng)膜內(nèi)的的電信號(hào)。瞳光反射反映形覺和時(shí)間通路，光感受器也包括視桿、錐和感光神經(jīng)節(jié)細(xì)胞，比ERG敏感范圍大結(jié)論：綜上所分析，ERG所反映的視網(wǎng)膜通路不與中心視力和視野、瞳光反射所檢查的通路。省立眼科醫(yī)院省眼科中華實(shí)驗(yàn)眼科雜志編輯部450003目的：綜述國(guó)內(nèi)重要眼科醫(yī)學(xué)期中醫(yī)學(xué)統(tǒng)計(jì)學(xué)方法的誤用和錯(cuò)誤描述情況，提出醫(yī)學(xué)設(shè)計(jì)時(shí)統(tǒng)計(jì)學(xué)方法的選擇思路。方法：對(duì)國(guó)內(nèi)醫(yī)學(xué)期 出的論著文章進(jìn)行閱讀，總結(jié)有關(guān)醫(yī)學(xué)統(tǒng)學(xué)方法的使用正確與否。結(jié)果：在醫(yī)學(xué)期中，統(tǒng)計(jì)學(xué)方法的誤用主要包括下列幾個(gè)方面：1普述；2對(duì)于兩組獨(dú)立設(shè)計(jì)資料無(wú)論樣本量大小均應(yīng)用獨(dú)立樣本t檢驗(yàn)方法處理數(shù)據(jù)資料，同時(shí)不注意數(shù)據(jù)資料是否滿足正態(tài)分布條件；3兩因素或多因素方差檢驗(yàn)設(shè)計(jì)用單因素方差分析進(jìn)行統(tǒng)計(jì)學(xué)分析；4大于兩個(gè)水平的定量資料簡(jiǎn)單地應(yīng)用t驗(yàn)進(jìn)行統(tǒng)計(jì)學(xué)分析，增加了I錯(cuò)誤率；5方差分析后的組間多重比較不對(duì)方法進(jìn)行描述，甚至簡(jiǎn)單地將方差分析與多重比較混為一潭；6直線相關(guān)分析資料與回歸分析，且不提供散點(diǎn)圖，也不提供回歸方程；7將獨(dú)立分組的不同時(shí)間的檢測(cè)指標(biāo)用重復(fù)測(cè)試的方差分析進(jìn)行統(tǒng)計(jì)學(xué)處理；8對(duì)樣本量小的定性數(shù)據(jù)資料簡(jiǎn)單應(yīng)用卡方檢驗(yàn)進(jìn)行處理；920樣本量資料應(yīng)用相對(duì)數(shù)結(jié)論：國(guó)內(nèi)眼科醫(yī)學(xué)期中醫(yī)學(xué)統(tǒng)計(jì)學(xué)方法的誤用現(xiàn)象極為普遍，應(yīng)該按照研究設(shè)計(jì)選擇相對(duì)正確的統(tǒng)計(jì)學(xué)方法。陳中山宋艷萍廣州總醫(yī)院、書寫、醫(yī)囑、手術(shù)通知等整合為一個(gè)系統(tǒng)。方法：應(yīng)用有線或無(wú)線網(wǎng)絡(luò)，講眼科檢查設(shè)備，影像檢查如眼底彩照、OCT、FFA和ICG、B超、UBM等，與功能檢查如視覺電生理、視野、微