Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEDeoxycholicacidCat.No.:HY-N0593CASNo.:83-44-3Synonyms:CholanoicAcid;Desoxycholicacid分?式:C??H??O?分?量:392.57作?靶點(diǎn):Gprotein-coupledBileAcidReceptor1;EndogenousMetabolite作?通路:GPCR/GProtein;MetabolicEnzyme/Protease儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:≥100mg/mL(254.73mM)H2O:<0.1mg/mL(ultrasonic;warming;heatto80°C)(insoluble)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.5473mL12.7366mL25.4732mL5mM0.5095mL2.5473mL5.0946mL10mM0.2547mL1.2737mL2.5473mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:2.5mg/mL(6.37mM);Suspendedsolution;Needultrasonic2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(6.37mM);Clearsolution3.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(6.37mM);ClearsolutionBIOLOGICALACTIVITY?物活性Deoxycholicacid(cholanoicacid)?種膽汁酸，腸道代謝副產(chǎn)物，可以激活G蛋?偶聯(lián)膽汁酸受體TGR5。IC50&TargetMicrobialMetaboliteHumanEndogenousMetabolite體外研究Deoxycholicacid(DCA)(100μM)inducestheproductionofgastriccancercelllineMGC803resistanttoacidifiedbileacidsandenhancestheirsurvivalandproliferationactivityunderbileacidstress[2].Deoxycholicacid(DCA)(100μM)-inducedresistantcellsshowsalteredmorphology,significantlyelevatedtelomeraseactivity,bettercellviabilityandreducesapoptosiscomparedtonormalMGC803cells[2].PROTOCOLCellAssay[2]MGC803cellsareculturedinRoswellParkMemorialInstitutemediasupplementedwith10%fetalcalfserumand100U/mLPenicillinand100mg/mLStreptomycin.TogenerateMGC803-resistantcells,thepHvalueoftheMGC803culturemediumisadjustedtotheexperimentalconditionsusingthehydrochloricacid(A).ThebileacidsGCDAandDeoxycholicacidaredilutedtooptimalworkingconcentrationsof100μM(B)withculturemedium,andtheoverallpH(A+B)isadjustedtopH5.5,simulatingthegastricenvironment.Initially,MGC803cellsarechronicallyexposedtoacidifiedmediumwithbileacids(A+B)for10minevery24h.Theexperimentaltimeandconditionsareoptimizedinourpreliminaryexperiments,whichshowthat10minisenoughanddoesnotresultincelldamage.Thisprocedureisrepeatedandittakes60weeksfortheMGC803cellstosurviveandproliferateundertheexposureofA+Bfor120min.ControluntreatedcellsareculturedinneutralRPMImediumatpH7.4inparalleltotheresistantcellsfor60weeks.ThemorphologicalchangesinMGC803cellsexposedtoacidifiedbileacids(A+B)aredocumentedat30and60weeks[2].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?CellRes.2019Mar;29(3):193-205.?NatCellBiol.2018Oct;20(10):1145-1158.?Microbiome.2019Mar20;7(1):43.?JInvestDermatol.19November2021.?CellDeathDiscov.2020Jul6;6:56.2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESeemorecustomervalidationsonwww.MedChemEREFERENCES[1].SommE,etal.β-Klothodeficiencyprotectsagainstobesitythroughacrosstalkbetweenliver,microbiota,andbrownadiposetissue.JCIInsight.2017Apr20;2(8).pii:91809.[2].WangX,etal.Acidifiedbileacidsenhancetumorprogressionandtelomeraseactivityofgastriccancerinmicedependentonc-Mycexpression.CancerMed.2017Apr;6(4):788-797.McePdfHeightCau