Hi,hello,professor,It’sapleasuremeetingyouThankyousomuchforgivingmethisopportunity.自我介紹（canyouintroduceyouself）Thankyouforgivingmethechancetointerviewanditismypleasuretointroducemyselftoyou.MynameisXX.ImajorinXX，andIgraduatedfromtheXXMedicalUniversityinJune,2015.NowIamworkingasaresidentinXXPeople’shospital.Duringthepastyears,understrictguidanceofmytutor,professorXXX,IhavelearnedsystematicallytheoryofprofessionandacquirethebasicmanipulativeskillsaboutXX.Withthehelpofmysupervisor,Ihavesuccessfullyfinishedthesubject“XX”andgraspedsomeexperimentalskills,suchasDNAextractionandauditoryelectrophysiology,buildinganimalmodelsofotologyandsoon.ItriedmybesttolearnMedicalEnglishandmasteredalotofprofessionalvocabulary.Iamgoodatlistening,speaking,readingandwriting..IcanmanipulatecomputerproficientlyandmasterMicrosoftOfficesoftware,alsobefamiliarwithSPSSandPhotoshop,etc.Duringthethree-yearstudyinresearch,Istudiedhardandstrivedforexcellenceinourfield.Ibenefitfromworkingtogetherwithexcellentcolleagues.Idevelopedqualityofdiligence,responsibility,kindnessandhonesty.MyinternshipinTheFirstAffiliatedHospitalofXXMedicalUniversitycouldqualifymeforthisjob,andIbelieveIwilladapttothepositionquickly.Iamlookingforwardtoworkinginyourdepartment.Thankyou2、為什么讀博士(Whydoyouwanttogetamedicaldoctordegree？)Firstofall,ithasalwaysbeenmydreamtobecomeanexcellentdoctor..Throughpursuingadoctor'sdegreeIcanbetterpreparetotreatmypatients:andturnmyknowledgeintoresultsandachievements.Moreover,Icantreatproblemsfromahigherstandpoint,andgetdeeperknowledgeofmyfields,alsoIcanlearnthelatestfrontresearchofmymajor,whicharesignificantinmyfutureresearch.It'smoreabouttrainingagrowthmindsetandcriticalthinking.Secondly,asaresident,jobclassificationandtreatmentimprovingarecloselylinkedtotheascensionofdegree,itisthemostrealisticreasonthatpromptmetoreceivetheDr.degreeeducation.AlthoughIamafemale,Istillwanttodotheworkwell,beeconomicindependenceandcontributetomyfamily.Themostimportantformeisthesupportfrommyfamily.Ihaveafairlygoodfamily,myparentsrespectmychoiceanddream,anddotheirbesttosupportmefromeveryaspect.TogetaPhDdegree,Icouldbewithagroupofthesamelevel,thesamerealmofintimateclassmateshandinhand,thislifeisnolongerlonely.Differentlevelsoflearningwithinadisciplinecanleadtodifferentworlds.Theworldisoutthere,andwecan'tchangeit.Butwecanlearn,becausehowmuchknowledgewehave,howourworldis.Inconclusion,thereasonIpursuemyPh.D.hasbeenclarifiedabove,andIwillconcentrateonmylearningprocess,drawfullattentiontowhatIwillstudyeveryday,andalsoenjoytheprocessofrealizingmydream.Inaword,Ilikethefeelingofgrowingupeveryday.3.為什么選擇我們（Whydoyouchooseourgroup）Iamdeeplyimpressedbytheacademicatmospherewhenconnectingtothewebsiteofyourdepartment.Inaddition/besides,Iamattractedbytheresearchofyourteam.IfIgotachancetostudyhere,Iwouldliketocontinuetoacceptprofessionaltraining,inordertogetreadyforworkinginthefuture.IhopeIcanachievemygoalsoon.4.博士研究的規(guī)劃(Planningfordoctoralresearch)IwanttoexploretheresearchtopicinExosomesfromdrug-resistantNasopharyngealcarcinomacellstransmitchemo-resistancebyahorizontaltransferofmicroRNAs.Drugresistanceofnasopharyngealcarcinomacellsisoneofthemainreasonsforthefailureofchemotherapyandhighrecurrencerate.TheObjectiveistoextractandidentifyexosomesfromnasopharyngealcarcinomacells.Extractionandidentificationofexosomesfromhumannasopharyngealcarcinomacellsandcisplatinresistantcellswillbeextractedbykitandultra-highspeedcentrifugation.Themorphologyandsizeofexosomeswillbeobservedbytransmissionelectronmicroscopy,andtheexpressionleveloftransmembranesomeproteinofexosomeswillbedetectedbyWesternBlot.Extracellularexosomeswillbeextracted,whicharequantified,andtheexosomescouldenterthecellsthroughendocytosiswillbeobservedbylaserconfocalmicroscopy.Therefore,thisstudywilltakeexosomesderivedfromNPCcellsastheresearchobjecttoexploretherelationshipbetweenexosomesandtumordrugresistanceofNPCcellsandtheirrelatedMIRNA,aswellastheirpossiblemechanismofaction,soastoprovideanewideaforgenetherapyoftumormulti-drugresistance.Themechanismsofdrugresistanceincludeapoptosisinhibition,highexpressionofdrug-resistantrelatedproteinsandabnormalexpressionofmicroRNAs.Exosomeisakindofcellsecretednanoscalevesicle,richinavarietyofRNAs,lipidsandproteins,whichcanbeusedasinformationcarriertotransfersubstancesandinformationbetweencells.Exosomessecretedbytumorcellsarecloselyrelatedtotumorgrowth,metastasis,immunetoleranceanddrugresistance.Inaddition,severalmicroRNAshavebeenreportedtoberelatedtodrugresistanceoftumors.Forexample,MIR-302,Mir-369,MIR-200C,Mir-34a,Mir-222,etc.5.你之前/現(xiàn)在的課題研究工作介紹（Introduceyourpastresearch）Ihavedone4researchesbefore,suchasXX,genechiptechnologyingenediagnosisandclinicalapplicationofnon-syndromedeafness.RESEARCHEXPERIENCEdissertationtopicMydissertation,wasaboutXXXProjectbriefintroduction:Toestablishascreeningplatformbasedonthetheorysystemofdeafnesscausedbygenemutation,toidentifythecauseofhereditarydeafness,andtoprovidestrictmedicalguidancefordeafpatientsinOurprovince.Inparticular,aminoglycosideantibioticsareprohibitedindeafpatientsandtheirfamilymembers,sothattheycanavoidserioushearingdisabilities.Toprovidegeneticcounselingandguidancefordeafcouplestoavoidtheformationofaspousewiththesamegenotypeofdeafchildren;Tocooperatewithprenataldiagnosisandneonatalscreening,addgeneticdiagnosisofhearing,reducethemutationrateofgenesorlocithatcausedeafnessinourprovince,soastoreducetheincidenceofdeafness.Throughscreeningandpedigreeanalysis,thegenebankofdeafnessmutationinXXprovincewasestablishedtolayafoundationforfurtherresearch.Yourresponsibilityinthisproject:dotheexperimentanddataanalysis你能說(shuō)說(shuō)你會(huì)的實(shí)驗(yàn)技術(shù)嗎（Canyoutellmeaboutyourexperimentaltechnique）proficiencyinDNAextraction,electrophysiologicalTDTsystemoperation,otologyanimalmodelbuilding,stereotaxiclocalizationofbrain,electricitydamagetechnique,Fluoro

Gold

(FG)

retrograde

tracingtechnique,你能說(shuō)說(shuō)其中一些具體的技術(shù)嗎(Canyoutellmesomespecifictechniques?)DNAextraction：Peripheralvenousblood2ml,3.2%sodiumcitrateanticoagulant,sterileat4℃，Saveforinspection.BloodproducedbyTiangenBiochemicalTechnologyCo.,LTDGenomicDNAextractionSystem(non-centrifugalcolumn)(ArticleNo.:DT319-02),crystalcoreBiomix-ERtMIIchiphybridinstrument,crystalcoreSlideWasherTMchipdesiccant,LuxScanTM10K-BmicroarraychipscannerbyBeijingBoaoBioengineeringCo.,LTDThecompanyprovides.GenomicDNAwasextractedfromperipheralbloodofallpatientsWithTheCONCENTRATIONandpurityofDNAweredetectedbyanULTRAVIOLETspectrophotometer,andtherestwerepreservedSetasideinrefrigeratorat-70℃.6.你不工作的時(shí)候干嘛(Whatdoyoudowhenyou'renotworking)Makingcookies,bakingcakes,learninglocaldishes,singing,watchingmovies,doingsportsandalsoshopping7.你在工作中遇到困難怎么辦(Whatdoyoudowhenyouhaveadifficultwork)Iwillrefertotheliteratureandsearchontheinternetfirst，ifitistoodifficulttofigureout,Iwilltrytoseekforhelpfrommyteammemberoramentor求助誰(shuí)？(Whowouldyouaskforhelp)ToAteammember,amentorforhelp8.學(xué)習(xí)過(guò)的相關(guān)課程(whatrelevantcourseshadyoulearned)basicchemistry,computerapplication,medicalphysics,systematicanatomy,histoembryology,clinicalimmunology,physiology,pathobiology,cellbiology,oncology,animalexperiment,pharmacology,medicalstatistics,clinicalepidemiology,medicalmolecularbiology,andsoon.9.社會(huì)活動(dòng)/交往能力(Socialactivities/communicationskills)Iamapostive,sociablepersonandjoinedvarioustypesofactivitiesfromschoolandhospital,I’vebeenthemassageteamleader,ledtheteamtotheoldpeople'shomeonceparticipatedinthefreeclinicactivities,duringtheundergraduateperiod,Astheprojectteamleader,Iledtheteamtoobtainscientificresearch.duringthepostgraduateperiod,asamonitor,IorganizedstudentstoparticipateinfreeclinicactivitiesAfterwork,ItookpartinpublicwelfareactivitiesfordeafpatientsIWorkedasavoluntarydoctoratthehospital’sDepartmentofFeverduringthefightagainstCOVID-19inthisyear.10.你最近主要在干什么(Recently,whathaveyoubeendoing)Dailyclinicalworkandforeignlanguagelearning11.你能說(shuō)說(shuō)你的資金來(lái)源的情況嗎（Couldyoutellmesomethingaboutyourfund）ApplyCSCtogotoGermanyinAugustinthenextyear.CSCisChinesegovernmentfunding，thatisafundofChinesescholarshipcounciltosupportthestudenttogoaboard.CSCneedsomeapplicationmaterialsuchasyourinvitationletter,yourCV,andresearchproject.12.回國(guó)后想做什么工作