應(yīng)用遺傳大數(shù)據(jù)解析作物養(yǎng)分高效性狀的遺傳機(jī)制2019-8-5應(yīng)用遺傳大數(shù)據(jù)解析作物養(yǎng)分高效性狀的遺傳機(jī)制2019-8-511.遺傳大數(shù)據(jù)研究體系2.遺傳大數(shù)據(jù)與作物養(yǎng)分的結(jié)合3.遺傳大數(shù)據(jù)與作物養(yǎng)分結(jié)合的研究進(jìn)展報(bào)告提綱1.遺傳大數(shù)據(jù)研究體系報(bào)告提綱21.遺傳大數(shù)據(jù)研究體系2.遺傳大數(shù)據(jù)與作物養(yǎng)分性狀的結(jié)合3.遺傳大數(shù)據(jù)與作物養(yǎng)分性狀結(jié)合的研究進(jìn)展報(bào)告提綱1.遺傳大數(shù)據(jù)研究體系報(bào)告提綱3遺傳大數(shù)據(jù)方法解析玉米性狀的應(yīng)用從單一群體到多個(gè)群體結(jié)合遺傳與組學(xué)技術(shù)進(jìn)行復(fù)雜性狀的挖掘基因組信息表型遺傳學(xué)；育種學(xué)統(tǒng)計(jì)學(xué)；生物信息學(xué)遺傳大數(shù)據(jù)解析遺傳群體(自然群體，人工群體)0100200300400500200020102018YearNumberbiologicalbigdatabiologicalbigdataplant單一群體株型與產(chǎn)量性狀多群體多性狀多環(huán)境的系統(tǒng)解析遺傳大數(shù)據(jù)方法解析玉米性狀的應(yīng)用從單一群體到多個(gè)群體結(jié)合遺傳4創(chuàng)建玉米多親本隨機(jī)混合分離群體，熟悉基因組信息Panetal.,2016,NewPhytologist050100150(Mb)02468BzRecombinationrate(cM/Mb)ABD1234756891012347568910RecombinantsMaizeWholeGenomeMaizeChromosome9MaizeWholeGenomeChr.32110987654RecombinationLandscapeCMinMaxRecombinationRate(cM/Mb)B73/BY804BY815/KUI3KUI3/B77KUI3/SC55K22/CI7MO17/X26-4ZHENG58/SKZONG3/YU87-1DE3/BY815K22/BY815DAN340/K22YU87-1/BK創(chuàng)建玉米多親本隨機(jī)混合分離群體，熟悉基因組信息Panet5不斷挖掘新的基因組信息AGCTGGTGTCTAGGGGCCAGCTGGTGTCTAGGGGCCTAGCTGGTGTCTAGGGGCCGTAGCTGGTGTCTAGGGGCCTAGAGCTGGTGTCTAGGGGCCTCTAAGCTGGTGTCTAGGGGCCTAGGGAGCTGGTGTCTAGGGGCCAGCTGGAGCTGGTGTCTAGGGGCCTGTCTAGAGCTGGTGTCTAGGGGCCGTCTAGGG18nt19nt20nt21nt22nt23nt24nt25nt26ntALine1Line2Line3Line338……MaizeassociationpanelSequencingSequenceextraction376,780192,852197,75526,07230,975B73Mo17SmallRNAspeciesBSmallRNAcluster166,780B73Mo17366,440Panetal.,unpublished基因組GeneTFSmallRNACircleRNALongnocodingRNA不斷挖掘新的基因組信息AGCTGGTGTCTAGGGGCCA6創(chuàng)建了混合群體定位分析方法，克隆一個(gè)控制株型的新基因Panetal.,2017,PlantPhysiologyPHEHTALTBNULALNAELNBELLLW2年12點(diǎn)田間試驗(yàn)，10個(gè)株型性狀創(chuàng)建了混合群體定位分析方法，克隆一個(gè)控制株型的新基因Pan7利用上述群體與方法開(kāi)展了多個(gè)性狀的研究遺傳圖譜創(chuàng)新群體定位方法玉米基因組進(jìn)化籽粒維生素E遺傳解析玉米溫?zé)岽蚪M進(jìn)化籽粒大小遺傳解析籽粒初級(jí)代謝物遺傳解析

穗部性狀遺傳解析籽粒氨基酸含量遺傳解析進(jìn)化品質(zhì)品質(zhì)產(chǎn)量利用上述群體與方法開(kāi)展了多個(gè)性狀的研究遺傳圖譜創(chuàng)新群體定位方8利用遺傳大數(shù)據(jù)方法克隆了與綠色革命相關(guān)的新基因ZhangandPanetal.,2019,MolecularPlant

(Co-firstauthor）

5’UTRATGTAA6bpInsertionG1G2G3G4G5G6G7G8G9G10G11G12G13283032343638-log10(P)130140150Chr7(Mb)135145300kbGeneannotationEightexpressiongenesExpressiondifferencegene1gene3gene6gene7Expressionnodifferencegene4gene10gene12gene13Gene7-Zm00001d020874Nonsynonymousgene4gene12gene135‘UTRinsertiongene3gene6gene7HZS1462SNP2(T)SNP1(T)6bpDeletion5’UTRATGSNP2(G)SNP1(C)TAASNP3(G)SNP4(T)SNP5(A)SNP3(A)SNP4(C)SNP5(C)300kb40000050100150Chr7(Mb)Euclideandistanceraisedtothefourthpoweronchromosome7ED4利用遺傳大數(shù)據(jù)方法克隆了與綠色革命相關(guān)的新基因Zhanga9報(bào)告提綱1.遺傳大數(shù)據(jù)研究體系2.遺傳大數(shù)據(jù)與作物養(yǎng)分性狀的結(jié)合3.遺傳大數(shù)據(jù)與作物養(yǎng)分性狀結(jié)合的研究進(jìn)展報(bào)告提綱1.遺傳大數(shù)據(jù)研究體系10現(xiàn)代植物營(yíng)養(yǎng)遺傳學(xué)的契機(jī)1922玉米品種對(duì)肥力相應(yīng)差異1929營(yíng)養(yǎng)突變體1963對(duì)離子的吸收由遺傳控制1972《植物礦質(zhì)營(yíng)養(yǎng)原理》提出植物營(yíng)養(yǎng)學(xué)概要1994孟德?tīng)栠z傳規(guī)律基因?qū)W說(shuō)中心法則分子標(biāo)記QTL定位基因組遺傳大數(shù)據(jù)18651926195719952000-2000-2010-植物礦質(zhì)營(yíng)養(yǎng)學(xué),養(yǎng)分歸還學(xué)植物根際營(yíng)養(yǎng)18401984新時(shí)代植物營(yíng)養(yǎng)遺傳學(xué)關(guān)聯(lián)分析植物營(yíng)養(yǎng)遺傳學(xué)引入中國(guó)嚴(yán)小龍張福鎖1939營(yíng)養(yǎng)功能必需元素1972植物營(yíng)養(yǎng)遺傳現(xiàn)代植物營(yíng)養(yǎng)遺傳學(xué)的契機(jī)1922玉米品種對(duì)肥力相應(yīng)差異19211現(xiàn)代植物營(yíng)養(yǎng)遺傳：利用生物大數(shù)據(jù)系統(tǒng)解析植物營(yíng)養(yǎng)學(xué)問(wèn)題微生物元素根系葉片土壤不同環(huán)境條件下基因型植物營(yíng)養(yǎng)體系遺傳群體基因組信息組學(xué)技術(shù)發(fā)展植物營(yíng)養(yǎng)性狀的復(fù)雜性（植物-環(huán)境）現(xiàn)代遺傳學(xué)的突破性進(jìn)展（基因組學(xué)）個(gè)體性狀差異-動(dòng)態(tài)+多環(huán)境群體水平差異-動(dòng)態(tài)+多環(huán)境遺傳大數(shù)據(jù)方法-動(dòng)態(tài)+多環(huán)境挖掘候選基因，解決養(yǎng)分吸收利用高效協(xié)同機(jī)制養(yǎng)分吸收利用科學(xué)問(wèn)題研究思路研究需求遺傳個(gè)體現(xiàn)代植物營(yíng)養(yǎng)遺傳：微生物元素根系葉片土壤不同環(huán)境條件下基因型12研究方向：玉米氮營(yíng)養(yǎng)高效遺傳基礎(chǔ)氮高效基因型驅(qū)動(dòng)的植物-微生物互作機(jī)制氮高效利用與高產(chǎn)協(xié)同機(jī)制Geigeretal.,2005Zhangetal.,2019研究方向：玉米氮營(yíng)養(yǎng)高效遺傳基礎(chǔ)氮高效基因型驅(qū)動(dòng)的植物-微生13玉米氮高效品種的生理基礎(chǔ)：花后氮轉(zhuǎn)移過(guò)程是協(xié)同高產(chǎn)和氮高效的關(guān)鍵XY335中上部葉片中的氮轉(zhuǎn)移效率顯著高于ZD958（郭松，未發(fā)表）氮轉(zhuǎn)移關(guān)鍵基因GS1.4在XY335中上部葉片中的表達(dá)水平顯著高于ZD958玉米氮高效品種的生理基礎(chǔ)：XY335中上部葉片中的氮轉(zhuǎn)移效率14報(bào)告提綱1.遺傳大數(shù)據(jù)研究體系2.遺傳大數(shù)據(jù)與作物養(yǎng)分性狀的結(jié)合3.遺傳大數(shù)據(jù)與作物養(yǎng)分性狀結(jié)合的研究進(jìn)展報(bào)告提綱1.遺傳大數(shù)據(jù)研究體系15高產(chǎn)氮高效玉米親本吐絲期后存在氮利用效率差異先玉335親本個(gè)體在吐絲期后葉片氮含量與保綠度存在差異Gongetal.,unpublished先玉335親本在吐絲期后葉片保綠度存在差異高產(chǎn)氮高效玉米親本吐絲期后存在氮利用效率差異先玉335親本個(gè)1605001000150004812LOD(Mb)Chr1Chr2Chr3Chr4Chr5Chr6Chr7Chr8Chr9Chr102000…..…..PH4CVPH6WCDH1DH200…..Panetal.,2016,NewPhytologist;Panetal.,2017,PlantPhysiology結(jié)合遺傳大數(shù)據(jù)方法挖掘了控制氮利用效率的主效位點(diǎn)親本個(gè)體在吐絲期后葉片氮轉(zhuǎn)移存在差異構(gòu)建了親本差異的后代群體定位到氮轉(zhuǎn)移差異的主效位點(diǎn)構(gòu)建了后代群體的遺傳圖譜05001000150004812LOD(Mb)Chr1Ch17利用組學(xué)技術(shù)挖掘高產(chǎn)氮高效品種親本間差異基因Gongetal.,unpublished鑒定了近8000個(gè)吐絲后3個(gè)時(shí)期的上調(diào)與下調(diào)差異基因差異基因進(jìn)行了GO富集分析利用組學(xué)技術(shù)挖掘高產(chǎn)氮高效品種親本間差異基因Gonget18高產(chǎn)氮高效品種親本葉片差異表達(dá)基因與氮利用效率相關(guān)基因表達(dá)模式分為8個(gè)模塊，其中2個(gè)模塊與氮轉(zhuǎn)移表型相關(guān)Gongetal.,unpublished高產(chǎn)氮高效品種親本葉片差異表達(dá)基因與氮利用效率相關(guān)基因表達(dá)模19氮利用效率相關(guān)基因共表達(dá)模塊構(gòu)建鑒定出控制氮轉(zhuǎn)移核心調(diào)控基因Gongetal.,unpublishedRedmoduleHighTurquoisemodulePH6WCPH4CVPH4CVPH6WCLowBColorkey(-log10P)02.55.0bindingcarbohydratemetabolicprocesscatabolicprocesscatalyticactivitycellcommunicationcellcyclecellularaminoacidandderivativemetabolicprocesscellularcomponentorganizationcytoskeletoncytosolDNAmetabolicprocessendoplasmicreticulumhydrolaseactivitykinaseactivitylipidmetabolicprocessmembranemulticellularorganismaldevelopmentnucleaseactivitynucleobase,nucleoside,nucleotideandnucleicacidmetabolicprocessnucleusplasmamembraneplastidproteinbindingproteinmetabolicprocessresponsetoabioticstimulusresponsetohormonestimulusresponsetoextracellularstimulusresponsetostressripeningsecondarymetabolicprocesssignaltransductionthylakoidtranscriptionfactoractivitytranscriptionregulatoractivitytransportregulationofgeneexpression,epigeneticPH6WC0_1PH6WC0_2PH6WC0_3PH6WC15_1PH6WC15_2PH6WC15_3PH6WC25_1PH6WC25_3PH6WC25_2PH6WC0_1PH6WC0_2PH6WC0_3PH6WC15_1PH6WC15_2PH6WC15_3PH6WC25_1PH6WC25_3PH6WC25_2PH4CV0_1PH4CV0_2PH4CV0_3PH4CV15_1PH4CV15_2PH4CV15_3PH4CV25_1PH4CV25_2PH4CV25_3PH4CV0_2PH4CV0_3PH4CV15_1PH4CV15_2PH4CV15_3PH4CV25_1PH4CV25_2PH4CV25_3PH4CV0_1ACGRMZM2G137421GRMZM2G137421GRMZM2G104639GRMZM2G084397GRMZM2G005233GRMZM2G114893GRMZM2G044383GRMZM2G349187GRMZM2G087103GRMZM2G150758GRMZM2G393529GRMZM2G065829氮利用效率相關(guān)基因共表達(dá)模塊構(gòu)建鑒定出控制氮轉(zhuǎn)移核心調(diào)控基因20PH6WCSPH6WC15PH6WC25PH4CVSPH4CV15PH4CV25WRKY20/1WRKY4/62WRKY2/71WRKY4/58WRKY2/52WRKY72/26WRKY42/69WRKY21/12WRKY51/27WRKY33/57WRKY40/124WRKY51/73WRKY108/75WRKY22/47WRKY70/15WRKY53/79WRKY53/121WRKY53/38WRKYERFAMYBbHLHNACNAC079,080,4,080/40NAC047/122NAC047/25NAC41/63NAC90/93PH6WCSPH6WC15PH6WC25PH4CVSPH4CV15PH4CV25PH6WCSPH6WC15PH6WC25PH4CVSPH4CV15PH4CV25PH6WCSPH6WC15PH6WC25PH4CVSPH4CV15PH4CV25PH6WCSPH6WC15PH6WC25PH4CVSPH4CV15PH4CV25●●WRKYERF0.0900.0950.1000.105GeneRatio0.0050.0100.0150.0200.025p.adjustCount●50.052.555.057.560.00.00070.0262BCGRMZM2G114444AC203535.4_FG001GRMZM2G076657GRMZM2G064630GRMZM2G051793GRMZM2G003715氮轉(zhuǎn)移表型相關(guān)基因轉(zhuǎn)錄因子表達(dá)模式鑒定出氮轉(zhuǎn)移相關(guān)的核心轉(zhuǎn)錄調(diào)控因子Gongetal.,unpublishedPH6WCSPH6WC15PH6WC25PH4CVSPH4C2105001000150004812LOD(Mb)Chr1Chr2Chr3Chr4Chr5Chr6Chr7Chr8Chr9Chr102000利用組學(xué)結(jié)合大數(shù)據(jù)方法克隆了氮利用效率基因167.0(Mb)173.0(Mb)

130genesRNA-seq23DEGsbetweenPH6WCandPH4CVWGCNA11genesin“turquoise”module0510152025300102030DaysaftersilkingGRMZM2G057841(ZMAASR6)PH6WCPH4CV0123456780102030DaysaftersilkingGRMZM2G059671(AT5G03730.1/AtCTR1)PH6WCPH4CV02468101214160102030DaysaftersilkingGRMZM2G093716(AT5G22370.1/EMB1705,QQT1)PH6WCPH4CV024681012140102030DaysaftersilkingGRMZM2G118737(ZMINVAN2)PH6WCPH4CV05101520250102030DaysaftersilkingGRMZM2G136296AT5G07350.2(AtTudor1)PH6WCPH4CV01020304050607080900102030DaysaftersilkingGRMZM2G172230(AT5G51070.1/CLPD,SAG15)PH6WCPH4CV01234567890102030DaysaftersilkingGRMZM2G352431AT1G77300.1(ASHH2)PH6WCPH4CV00.511.522.533.50102030DaysaftersilkingGRMZM2G54077AT1G53570.1(MAP3KA,MAPKKK3)PH6WCPH4CV00.511.522.533.544.50102030DaysaftersilkingGRMZM5G814945PH6WCPH4CV05101520250102030DaysaftersilkingGRMZM5G844257PH6WCPH4CV