天柏金黃散的藥效學(xué)評價【摘要】目的：評價天柏金黃散對小鼠模型的抗炎鎮(zhèn)痛作用以及對膝骨關(guān)節(jié)炎大鼠模型的影響，為該醫(yī)院制劑的臨床推廣應(yīng)用提供實驗依據(jù)。方法：采用2種小鼠抗炎試驗（耳腫脹及毛細(xì)血管通透性試驗）和2種小鼠疼痛模型（醋酸扭體模型、甲醛致痛模型）評價天柏金黃散的抗炎鎮(zhèn)痛作用；然后采用注射木瓜蛋白酶合半胱氨酸（2:1）方法建立大鼠膝骨關(guān)節(jié)炎模型，造模成功后將大鼠隨機(jī)分為模型組、陽性對照氟比洛芬組、天柏金黃散組，另設(shè)正常對照組，各組給予相應(yīng)藥物或干凈紗布捆綁連續(xù)14d，定期測定大鼠膝關(guān)節(jié)直徑，末次給藥后1h，取大鼠軟骨進(jìn)行HE染色觀察形態(tài)學(xué)改變并進(jìn)行Mankin評分。結(jié)果：天柏金黃散對醋酸誘發(fā)的小鼠扭體反應(yīng)和甲醛致痛均有顯著的鎮(zhèn)痛作用(P<0.01)；并明顯抑制二甲苯引起的小鼠耳腫脹(P<0.05)、醋酸誘導(dǎo)毛細(xì)血管通透性增加(P<0.01)；與模型組比較，天柏金黃散能顯著改善KOA大鼠模型的膝關(guān)節(jié)腫脹程度和改善軟骨病變(P<0.01)。結(jié)論：天柏金黃散具有顯著的抗炎鎮(zhèn)痛作用，能明顯改善KOA大鼠模型及軟骨組織病理情況?！娟P(guān)鍵詞】天柏金黃散；藥效評價；膝骨關(guān)節(jié)炎；抗炎；鎮(zhèn)痛

PharmacodynamicevaluationofTianbaijinhuangsan[Abstract]Objective:Toevaluatetheanti-inflammatoryandanalgesiceffectofTianbaiJinhuangsanonmousemodelsandtheeffectonratmodelsofkneeosteoarthritis,whichprovidedexperimentalbasisfortheclinicalpromotionandapplicationofpreparationsinthishospital.Methods:Twomouseanti-inflammatorytests(earswellingandcapillarypermeabilitytest)andtwomousepainmodels(aceticacidtwistingmodelandformaldehydepainmodel)wereusedtoevaluatetheanti-inflammatoryandanalgesiceffectsofTianbaiJinhuangsan.Then,themodelofkneeosteoarthritisofratswasestablishedbyinjectionpapain-combinedcysteine(2:1),andaftersuccessfulmodeling,theratswererandomlydividedintomodelgroup,positivecontrolflurbiprofengroup,TianbaiJinhuangsangroup,andanothernormalcontrolgroup,eachgroupwasgiventhecorrespondingdrugorcleangauzebundlefor14days,thekneejointdiameterofratswasmeasuredregularly,and1hafterthelastadministration,ratcartilagewastakenforHEstainingtoobservemorphologicalchangesandMankinscore.KMmicewererandomlygroupedaccordingtobodyweight,andthemicewereadministeredcontinuouslyfor7days,andthemiceweresubjectedtoacetatetwisting,formaldehydepain,earswellingandcapillarypermeabilityexperimentstoevaluatetheanti-inflammatoryandanalgesiceffectofTianbaijinhuangsan.Results:TianbaiJinhuangsanhadsignificantanalgesiceffectsonaceticacid-inducedtorsionreactionandformaldehydepaininmice(P<0.01).Italsosignificantlyinhibitedxylene-inducedearswellinginmice(P<0.05)andaceticacidinducedincreasedcapillarypermeability(P<0.01).Comparedwiththemodelgroup,TianbaiJinhuangsansignificantlyimprovedthedegreeofkneeswellingandcartilagelesionsintheKOAratmodel(P<0.01).Conclusion:TianbaiJinhuangsanhassignificantanti-inflammatoryandanalgesiceffects,andcansignificantlyimprovethepathologicalconditionsofKOAratmodelandcartilagehistopathology.[Keywords]TianbaijinhuangsanEfficacyevaluationkneeosteoarthritisAnti-inflammatoryAnalgesia

目錄1前言 [1516]。我們的實驗結(jié)果基本與文獻(xiàn)報道吻合，可以明顯看出模型組關(guān)節(jié)囊膝關(guān)節(jié)腔形態(tài)發(fā)生了明顯的改變，而天柏金黃散具有治療膝骨關(guān)節(jié)炎的作用。

6結(jié)論通過采用2種小鼠抗炎試驗（耳腫脹及毛細(xì)血管通透性試驗）和2種小鼠疼痛模型（醋酸扭體模型、甲醛致痛模型）顯示天柏金黃散具有顯著的抗炎鎮(zhèn)痛作用；通過大鼠KOA模型研究,測定大鼠的關(guān)節(jié)腫脹度、行為學(xué)評分和組織病理形態(tài)學(xué)分析發(fā)現(xiàn)，天柏金黃散可以有效治療大鼠KOA。綜上所述，天柏金黃散具有良好鎮(zhèn)痛抗炎的作用，并且可以對大鼠KOA模型進(jìn)行有效治療，能夠為進(jìn)一步的臨床合理用藥和KOA新藥開發(fā)提供實驗依據(jù)。

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