Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEQNX-10Cat.No.:HY-175020分?式:C??H??FN?O?分?量:434.46作?靶點:FattyAcidSynthase(FASN);Apoptosis;Bcl-2Family作?通路:MetabolicEnzyme/Protease;Apoptosis儲存?式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY?物活性QNX-10?種具有抗癌活性的脂酸合酶(FASN)抑制劑(IC50=0.7μM)。QNX-10對結(jié)直腸癌和乳腺癌細胞表現(xiàn)出強效的FASN抑制和細胞毒性。QNX-10通過上調(diào)促凋亡蛋?Bax和下調(diào)抗凋亡蛋?Bcl-xL來誘導細胞凋亡(apoptosis)和細胞周期停滯于S期。QNX-10可?于研究針對FASN酶的抗癌療法[1]。IC50&TargetBaxBcl-xL體外研究QNX-10(0-10μM,48h)exhibitscytotoxicityagainstHCT-116,Caco-2,andMCF-7cellswithIC50of1.5μM,2.1μM,and1.8μM,respectively,andhasnotoxicitytoHEK-293cells,withanIC50of30.4μM[1].QNX-10(0.5-2.5μM,48h)inhibitscellcycleprogressionbyinducingSphasecellcyclearrestinHCT-116cells[1].QNX-10(0.5-2.5μM)increasesBaxanddecreasedBcl-xLandFASNlevels,exhibitingdose-dependentapoptoticpropertiesinHCT-116cells[1].CellCycleAnalysis[1]CellLine:HCT-116cellsConcentration:0.5μM,1.5μM,2.5μMIncubationTime:48hResult:InducedcellcyclearrestinSphase,resultingin23.3%and28.6%accumulationofthepopulationinSphaseat1.5μMand2.5μM,respectively,andincreasedtheG2/Mpopulationby27.5%and29.4%after1.5μMand2.5μMQNX-10exposure,respectively.ApoptosisAnalysis[1]1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemECellLine:HCT-116cellsConcentration:0.5μM,1.5μM,2.5μMIncubationTime:48hResult:Increasedthenumberofcelldeathcells,inducedcelldeathin15.4%ofthecellpopulation(10.93%apoptoticcells+4.47%necroticcells)at0.5μM,increasedthedeadcellpopulationto39.2%(31.6%apoptoticcells+7.6%necroticcells)at1.5μM,andinducedcelldeathto61.1%at2.5μM,ofwhich49.73%ofthecellsunderwentapoptosisand11.37%underwentnecrosis.REFERENCES[1].SinghS,etal.Design,synthesisandbiologicalevaluationof2-phenylquinoxalinecarbonylpiperazinederivativesasnovelFASNinhibitorswithanticanceractivity.BioorgChem.2025Jun21;163:108697.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonl