第1題Whichoneisnotclassifiedasvolatilepoison?ACyanideBParaquatCFormaldehydeDPhenol第2題Aconitumandbelladonnaaregaseouspoisons.第3題Inordertoensuretheefficacyandsafetyofdrugs,theresearchobjectofbioanalysisshouldonlyfocusonthehumanbody.第4題Whichoneisnotclassifiedastheanimalpoison?ACantharidinBTetrodotoxinCAconitumDToadtoxin第5題ThetaskofbiopharmaceuticalandtoxicologicalanalysisdoesnotincludeAPerformanalyticalmethodologystudyBProvidedataforpharmacokineticresearchCProvidedataforclinicaltherapeuticdrugmonitoringDFindandsolvequalityproblemsintheproductionprocessofdrugpreparations第6題Poisonisachemicalthatcandamagethenormalactivitiesoflifethroughphysicochemicalactionafterenteringtheorganism,causingdiseasesorevendeath.第7題AccordingtoArticle357oftheCriminalLawofthePeople'sRepublicofChina,drugsaretheaddictivenarcoticandpsychotropicdrugsthatcancauseaddiction.第1題TheprocessbywhichthedrugentersthebloodcirculatorysystemfromthesiteofadministrationwithoutchemicalstructuralchangeiscalledAAbsorptionBDistributionCBiotransformationDExcretion第2題Whichdosageformhasthefastestabsorptionrate?ACapsuleBTabletCSustained-releasetabletDSolution第3題Theeffectofadrugisdirectlyrelatedtothetotalconcentrationofthedrugatthereceptorsite.Boththefreedrugandtheplasma-proteinbounddrugcanreachthereceptorsiteandshowthepharmacologicaleffect.第4題Duetothelargeabsorptionareaofthegastricmucosasurfaceandthelongerretentiontimeinthestomach,mostdrugsaremainlyabsorbedthroughthestomach.第5題Whenadrugenterstheliverthroughtheintestineforthefirsttime,somedrugscanbemetabolizedbyintestinaljuiceorenterobacteriaenzymesontheintestinalwall,ordrugenzymesintheliver,thusreducingtheamountofdrugenteringthesystemiccirculation.Thiseffectiscalledfirstpasseffect.第6題Whenthepermeabilityoftissuebiomembranedoesnothaveabarriereffect,whichfactorwillberate-limitingforthedrugdistribution?ApKaofthedrugBTissuebloodperfusionrateCDissociationdegreeatphysiologicalpHDPlasmaprotein-binding

第7題Amongthefollowingstatementsaboutactivetrasport,whichoneiswrong?AThedrugcanbetransportedagainstelectrochemicalgradientandconcentrationgradient.BRequiretheinvolvementofdrugtransporters.CNoneedtoconsumeenergy.DHasahighdegreeofselectivityandcanbecompetitivelyinhibitedbysimilarsubstances.第8題Whereisthedrugmetabolismmainlycarriedout?ALiverBGastrointestinaltractCSkinDKidney第9題WhichonedoesnotbelongtothephaseIreaction?AOxidationBReductionCHydrolysisDGlucuronidation第10題Whenanewdrug(itisasubstrateofCYP3A4)istakenwiththegrapefruitjuiceatthesametime,drug-druginteractionmayoccur.Whichstatementiswrong?AArepresentsthenewdrugwhichistakenalone.BArepresentsthenewdrugwhichistakentogetherwithgrapefruitjuice.CGrapefruitjuiceisamoderateinhibitorofCYP3A4.DInhibitorsofthemetabolicenzymescanslowdownthemetabolismofthesubstratedrugs.第11題CYP450isakindofmixed-functionoxidasesystemmainlyfoundinhumanliver.Becauseitcancombinewithcarbonmonoxideinthereducedstateandhasamaximumabsorptionpeakat450nmwavelength,itiscalledcytochromeP450.第12題Afterasingleoraldoseof25mgofethylmorphine,theaverageplasmaconcentrationofethylmorphineintheweakmetabolizer(PM)groupwassignificantlylowerthanthatinthestrongmetabolizer(EM)group.Asaresult,thepharmacologicaleffectswereweakened.第13題Amongthefollowingfactors,whichonecannotdirectlyaffecttheformationofthereceptor-drugcomplex(RD)?ADrugconcentrationatthereceptorsite.BNumberofreceptorsperunitvolume.CTheaffinitybetweenthedrugandthereceptor.DpKaofthedrug.

第14題Whichpharmacokineticparametercouldrepresentthetotaldrugamountabsorbedbythebody?APeakconcentration(Cmax)BAreaunderconcentration-timecurve(AUC)CPeaktime(Tmax)DApparentvolumeofdistribution(Vd)第15題Inordertoensuretheeffectivenessandsafetyoftheclinicaluseofdrugs,therapeuticdrugmonitoringshouldbecarriedoutaslongasthepatient'sfinancialabilitypermits.

Assignment第1題Amongthefollowingbiosamples,whichoneiscommonlyusedinthedeterminationofdrugmetabolitesinthebody?ABloodBUrineCMuscleDSaliva第2題DeterminationofdrugconcentrationinurineshoulduseAAny-timedurineBMorningurineCUrineduringthedayDTimedurine第3題Mostdrugsproducesystemictherapeuticeffectsafterenteringthebloodcirculation.Thereisacertainproportionalrelationshipbetweenthedrugconcentrationatthesiteofactionandthedrugconcentrationintheblood.Therefore,theclinicaltherapeuticeffectofthedrugpreparationcanbeindirectlypredictedbymeasuringthedrugconcentrationintheblood.第4題ThefalsestatementisAThemostcommonlyusedanticoagulantinplasmapreparationisheparin.BThedrughardlybindstofibrin,sothedrugconcentrationinplasmaandserumisusuallythesame.CSincetheamountofplasmaproducedislessthanthatofserum,mostresearchersprefertouseserumsamples.DSerumsamplesshouldbeusedwhenanticoagulantscontainedinplasmainterferewithdrugdetermination.第5題Plasmaorserumshouldbeseparatedintimeafterbloodcollection,generallynotmorethan2hours,andthenstoredinarefrigeratororfreezerafterseparation.Inordertoreduceoperationstepsandavoiderrors,itshouldnotpackedintosmallvolumes.第6題Useliquid-liquidextractionmethodtoextractacertainbasicdruginthetissuesample.Iftherearestillfat-solubleinterferingsubstancesintheextract,theextracteddrug-containingorganicphasecanbeback-extractedwithasmallvolumeofaqueoussolution,whichshouldbe

AAlkalineBNeutralCAcidicDAnypHisOK.第7題Useliquid-liquidextractionmethodtoextractacertainalkalinedrugwithpKa7.8inurine.WhatpHoftheurinesamplesshouldbeadjustedto?A5.8B7.8orlessC4.8orlessD10.8orabove10.8第8題10%trichloroaceticacidor6%perchloricacidsolutioncanbeusedtoprecipitateproteins,butthesupernatantisstronglyacidic(pH≤4),andthusisnotsuitablefordrugsapttodecomposeunderacidicconditions.第9題Biologicalsamplesshouldbeextractedunderacidicconditionstoreducetheinterferenceofendogenoussubstances(mostlyacidic)inthebiologicalmatrix.第10題Whichmethodcouldbeusedtodeterminethefreeaspirinconcentrationinplasma?AProteinprecipitationmethodBLiquid-liquidextractionCUltrafiltrationDSolidphaseextraction第11題TheproductformedbythecombinationofadrugoritsphaseImetaboliteswithendogenoussubstancesinthebodyiscalledphaseIImetabolitesorconjugates.Commonendogenoussubstancesareglucuronicacid,sulfuricacid,glycine,glutathioneandaceticacid.第12題ThemainpurposeofthechemicalderivatizationmethodinHPLCis:①ImprovethesensitivityofHPLCdetection;②Improvethevolatilityofdrugs.Themainderivatizationreactionsincludesilanization,esterification,acylation,andhalogenationderivatization,amongwhichsilanizationisthemostwidelyused.第13題Todeterminethetotalbloodconcentrationofwater-solubledrugs,whichmethodshouldbeused?ASolidphaseextractionBEnzymatichydrolysisCLiquid-liquidextractionDUltrafiltrationAssignment第1題Whenmeasuringbloodsamplesandtissues,alargeamountofproteinshouldberemovedfirst,andthedrugshouldbereleasedfromtheprotein-boundstate.第2題Whendeterminingsampleswithlowdrugconcentrations,itisadvisabletousetheextraction-concentrationmodeandhighlysensitiveanalyticaltechniques,suchasUVspectormetry.第3題Whatkindofpretreatmentmethodisnotsuitableforthestronglypolarorhydrophilicdrugs?ALiquid-liquidextractionBProteinprecipitationbyorganicsolventCProteinprecipitationbystrongacidDSolidphaseextraction第4題ThepreferredmethodfortheanalysisofsmallmoleculesinbiosamplesisAImmunoassayBHPLC-MSCBiologicalmethodDSpectroscopy第5題Whichonedoesnotbelongtothevalidationofbioanalyticalmethod.AStandardcurve

BAccuracyandprecisionCStabilityDForceddegradation第6題Whichitemcouldprovethatthesubstancemeasuredbytheproposedmethodistheexpectedanalyte.AStandardcurveandquantitativerangeBMatrixeffectCStabilityDSpecificity第7題Forthebioanalyticalmethod,thestandardcurveoflinearmodeshouldcontainatleast5concentrationpointsinadditiontothezeropoint.第8題Inthestudyofpharmacokineticsandbioavailability,LLOQshouldbeabletodeterminethedrugconcentrationinbiosamplesat3-5eliminationhalf-livesafterdosingorwithintherangeof1/5to1/2oftheCmax.第9題Theextractionrecoveryisusedtoevaluatetheextractionabilityofthesampleprocessingfromthebiosamples.Thevalidationfocusesonwhethertheanalytecouldbecompletelyextracted.第10題Theapplicationofstableisotope-labeledinternalstandardcannotonlyoffsetthematrixeffectduringmassspectrometryionization,butalsoeliminatethedifferenceinthesamplepreparationprocess.

第11題Whichoneisnotpartofthestabilitytestduringthevalidationofabioanalyticalmethod?ALong-termstoragestabilityBShort-termroomtemperaturestabilityCFreeze-thawstabilityDMobilephasestability第12題WhenusingLC-MStoanalyzebiosamples,itisnecessarytoevaluatewhethertheanalyteandtheco-eluatederivedfromthematrixcompeteforionizationattheLC-MSinterface.Whatisthisvalidationitemcalled?AAccuracyBStabilityCExtractionrecoveryDMatrixeffectAssignment第1題ThedifferencebetweentheAPCIandESIisAInAPCI,theanalyteabsorbsthelightemittedbythevacuum-ultravioletlampinthegasphaseandthenisionizedbyreleasingelectrons.

BAPCIhasnocoronadischargeneedle.CESIusescoronadischargetoionizethemobilephase.DComparedwithESI,APCIhasevaporatorandcoronadischargeneedle.第2題InLC-MS,whichreagentcannotbeaddedintothemobilephase?AAmmoniumacetateBFormicacidCAmmoniaDDisodiumphosphate第3題ESIissuitabletoanalyzethecompoundwithmediumtostrongpolarity,especiallythosethatcanformionsinthesolvent.第4題APCI

ismoresuitablefortheanalysisofioniccompounds.第5題TheAPCIionsourceisnotsuiblewhenanalyzingpeptides.第6題InLC-MS,non-volatileregentscanbeaddedintothemobilephase.第7題WhenusingLC-MStomeasurealkalinesubstances,thepHvalueofthemobilephaseshouldbelessthanpKa-2,andtheMSshouldbeworkedinthenegativeionmode.第8題IntriplequadrupoleLC-MS/MS,whichmodeshouldbeusedforthequantitativeanalysis?AProductionscanBPrecursorionscanCSelectivereactionmonitoringDNeutrallossscan第9題WhenanalyzingbiosamplesbyLC-MS,whichonecannoteliminatethematrixeffect?AChangetheionizationinterfaceBIncreasetheinjectionvolumeCOptimizethesamplepretreatmentmethodDAdjustthemobilephasecomposition第10題InLC-MS/MS,productionscanningismainlyusedforthequantitativeanalysis.第11題InLC-MS,thenegativeionmodeisusedforalkalinesubstances.

第12題Generallyspeaking,thedirectionofallparticlesincapillaryelectrophoresisisdeterminedbyAParticlesizeBParticleshapeCIonicstrengthDElectroosmoticflow

第13題TheprofileoftheelectroosmoticflowinHPCEandthatofthemobilephaseinHPLCareABothareadvection.BBotharelaminarflow.CTheformerisflatflow,andthelatterislaminarflow.DTheformerislaminarflow,andthelatterisflatflow.

第14題Inordertoimprovetheselectivityandresolutionofmicellarelectrokineticcapillarychromatography,optimizationmethodsincludeAChoosethetypeandconcentrationofmicelles.BChangethetypeofbuffer.CChangetheionicstrength.DChangethebuffercompositionandpH.

正確答案：ABCD第15題Whichfactorscouldaffecttheelectrophoreticmobilityofaprotein?ANetchargeBWeightCParticlediameterDThedifferenceinshape正確答案：ABCD第16題Whatarethefactorsaffectingelectroosmoticflowincapillaryelectrophoresis?AElectricfieldstrengthBBufferconcentrationCTemperatureDBuffertype

正確答案：ABCDAssignment第1題Whichofthefollowingisnotanadvantageofradioimmunoassay？AItcandetecttraceantigenandhapten.BItcandetecttraceantibody.CHighsensitivity.DHighspecificity.第2題Whichoneaboutthestandardforradioimmunoassayisright?AIthasthesameimmuneactivityandnoequalaffinityasthetestedsample.BIthasthesameimmuneactivityandaffinityasthetestedsample.CIthasdifferentimmuneactivityandaffinitycomparedwiththetestedsample.DIthasdifferentimmuneactivitybutsameaffinityasthetestedsample.第3題Whatarethenecessaryregentsforacompleteradioimmunoassay?ASpecificantigen,labeledantibodyBLabeledantigen,standard,specificantibody.CSpecificantibody.DLabeledantigen,

standard.第4題Whichoneisthecorrectdescriptionaboutthedisadvantegeofradioimmunoassay?APoorsensitivityBLowspecificityCPoorrepeatabilityDRadioactivecontaminationofnuclides第5題Basedonwhatcouldenzymeimmunoassaybedividedintohomogeneousandheterogeneoustechniques?AAntigeniccharacteristics.BAntibodycharacteristics.CWhetheritisnecessarytoseparatebindingandfreemarkersafterantigen-antibodyreaction.DStabilityofenzyme.第6題Whichofthefollowingdescriptionoffluorescentmarkersfortime-resolvedfluorescenceimmunoassayarecorrect？AThemarkerisinstable.

BLanthanidechelatecouldbeusedasamarker.

CThefluorescencedecaytimeofsomeproteinsinbiosamplesin1-1000microseconds.

DItcaneffectivelyeliminatetheinterferenceofnon-specificbackgroundfluorescence.正確答案：BD第7題IntermsoftheadvantagesofCEIA,whichoneisnotcorrect?ASomeproteincomponentscouldbeseparatedaccordingtotheircharge,andthenreactedwithantibody.BCEIAneedsmallamountofthesample.

CThefreeandbindingformsofimmunecomplexescanbeobserveddirectly.DItcannotsolvethefalsepositiveproblemcausedbycrossreactioninimmunereaction.第8題Capillaryelectrophoresis-basedimmunoassayisabbreviatedasACIEFBCEIACCITPDMECC第9題Amongthefollowingdetectionmethods,whichcouldbeusedforthecapillaryelectrophoresis-basedimmunoassay?AUVdetection.BLaser-inducedfluorescencedetection.CParticle