1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemETinoridine hydrochlorideCat. No.: HY-111354CAS No.: 25913-34-2Synonyms: Y-3642 hydrochloride分式: CHClNOS分量: 352.88作靶點(diǎn): Glutathione Peroxidase作通路: Metabolic Enzyme/Protease儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months

2、-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 31 mg/mL (87.85 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 2.8338 mL 14.1691 mL 28.3382 mL5 mM 0.5668 mL 2.8338 mL 5.6676 mL10 mM 0.2834 mL 1.4169 mL 2.8338 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。BIOLOGICAL ACTIVI

3、TY物活性 Tinoridine hydrochloride種甾體抗炎藥，也具有強(qiáng)效的由基清除和抗過(guò)氧化物活性。體外研究Tinoridine reduces a stable free radical, diphenyl-p-picrylhydrazyl, in the molar ratio of about 1:2, indicatingits free radical scavenging ability. Tinoridine inhibits the lipid peroxidation in rat liver microsomes induced byxanthine-xanth

4、ine oxidase system in the presence of ADP and Fe2+, in which hydroxyl radical is formed.1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemETinoridine is demonstrated to be oxidized in the course of the lipid peroxidation by following the fluorescencederived from the oxidation product of tinoridine. It

5、 is also oxidized by the xanthine-xanthine oxidase system inthe presence of Fe2+, but its oxidation is slow in the absence of Fe2+ and almost completely inhibited bycatalase. Tinoridine is also oxidized by H2O2-Fe2+ system producing OH (Fenton reaction), but it does notaffect the reduction of cytoch

6、rome c caused by superoxide radical 1.體內(nèi)研究 CCl4 aministration produces a marked decrease in the concentrations of liver microsomal cytochrome P-450and G6Pase, indicating that hepatic endoplasmic reticulum function is disrupted. Prior treatment of theanimals with tinoridine (100 mg/kg) significantly

7、reduces the CCl4-induced alterations in the enzymeactivities, and a rapid recovery toward the normal values is observed 2.PROTOCOLKinase Assay 2 CCl4 aministration produces a marked decrease in the concentrations of liver microsomal cytochrome P-450and G6Pase, indicating that hepatic endoplasmic ret

8、iculum function is disrupted. Prior treatment of theanimals with tinoridine (100 mg/kg) significantly reduces the CCl4-induced alterations in the enzymeactivities, and a rapid recovery toward the normal values is observed 2.MCE has not independently confirmed the accuracy of these methods. They are

9、for reference only.Animal Rat: Male Wistar rats (180-220 g) are used in the experiments. Drugs (Tinoridine) are given orally as aAdministration 2 suspension in 0.5% methylcellulose solution 1 hr before CCl4, administration. Control animals receive anequivalent amount of the vehicle. CCl4 is administ

10、ered ip at a dose of 0.25 ml/kg as a 5% (v/v) solution inolive oil. The animals are killed by carotid excision at different times after CCl4 administration; the livers arerapidly removed, weighed and processed for biochemical or histologic analysis 2.MCE has not independently confirmed the accuracy

11、of these methods. They are for reference only.REFERENCES1. O Shimada, et al. Hydroxyl radical scavenging action of tinoridine. Agents Actions. 1986 Nov;19(3-4):208-14.2. Yasuda H, et al. The protective effect of tinoridine against carbon tetrachloride hepatotoxicity. Toxicol Appl Pharmacol. 1980 Mar15;52(3):407-13.McePdfHeightCaution: Product has not