1、Chapter 8 Cell Adhesion Molecules(CAM)Jianing Wang ContentsPart Membrane molecules of immune cell Part Definition of leukocyte differentiation antigen and CD Part Definition, classification and functions of adhesion moleculesPart Clinical application of CD and adhesion moleculesCell Adhesion Molecul

2、esPart Membrane molecules of immune cell Receptors: TCR, BCR, CR, CKR, FcRClassand class MHC moleculesCD molecules: CD1350Cell Adhesion MoleculesPart Definition of leukocyte differentiation antigen and CDLeukocyte differentiation antigen(LDA): Cell surface molecules expressed (or disappeared) during

3、 different developmental and differential phases, activation or inactivation process of blood cells.Part Definition of leukocyte differentiation antigen and CDCD: cluster of differentiation. The same differentiation antigen recognized by different monoclonal antibody from different lab are called CD

4、.Human CD Groups GroupsCDT cellCD2, CD3, CD4, CD5, CD8, CD28, CD152, CD154, D272,CD278 ,CD294B cellCD19, CD20, CD21, CD40, CD79a, CD79b, CD80, CD86, CD267, CD268, CD269, CD307Myeloid cellCD14, CD35, CD64, CD256, CD257, CD312NK cellCD16, CD56, CD94, CD158, CD161, CD314, CD335, CD336, CD337Non-pedigre

5、eCD30, CD32, CD45RA, CD45RO, CD46, CD55, CD59, CD279PlateletCD36, CD41, CD51, CD61, CD62PAdhesion MoleculeCD11a-CD11c, CD15s, CD18, CD29, CD49a-CD49f, CD54, CD62E, CD62L, CD324, CD325, CD326CK/Chemotaxin RCD25, CD95, CD178, CD183, CD184, CD195, CD261-264Endothelial cellCD105, CD106, CD140, CD144, CD

6、299, CD309, CD321, CD322CarbonhydrateCD15s, CD60a-CD60c, CD75DCCD85, CD273, CD274-CD276, CD302, CD303, CD304Stem cell/Progenitor cellCD133, CD243Stromal cellCD292, CD293, CD331-CD334, CD339ErythrocyteCD233-CD242 CDs which take part in T cell recognition, adhesion and activation CDs which take part i

7、n B cell recognition, adhesion and activationPart Definition, classification and functions of adhesion molecules . DefinitionCell adhesion molecule (CAM) is a diverse family of extracellular and cell surface glycoproteins which involve in cell-cell and cell-extracellular matrix adhesion, recognition

8、, activation, and migration.All those molecules are integral membrane proteins which have cytoplasmic, transmembrane and extracellular domains . ClassificationIntegrin familySelectin family Immunoglobulin (Ig) superfamilyCadherin family Mucin-like familyOther adhesion molecules1. Integrin familyInte

9、grins consist of and chains.According to subunits, Integrins are divided into eight groups: 1- 8VLA-4(Very Late Antigen-4)-VCAM-1 LFA-1(Lymphocyte Function-associated Antigen-1)-ICAM-1,2,3 Integrin and platelet aggregation(Karp, 2001)Integrins promote immune response2. Selectin familySelectins consi

10、st of one peptide chain.The three family members include: E-selectin, L-selectin, and P-selectin.3. Ig superfamily(IgSF) The structure of these adhesion molecules resemble that of Ig.CD4, CD8, CD2(LFA-2), CD58(LFA-3), VCAM-1, ICAM-1,2,3 4. Cadherin family E-cadherin- Epithelia cellN-cadherin- Nerve

11、cellP-cadherin-Placenta 5. Mucin-like family CD34, GlyCAM-1(glycosylation dependent cell adhesion molecule-1) PSGL-1(P-selectin glycoprotein ligand-1) 6. Other adhesion molecules CD44. FunctionsParticipate in development and differentiation of immune cellsCD2-LFA-3LFA-1-ICAM-1-Participate in develop

12、ment and maturation of thymocytes.2.Participate in immune response and regulation3. Participate in the adhesion of leukocyte and vascular endothelial cell during inflammation. 4. Participate in lymphocyte homing Lymphoid stem cell migrate to central lymphoid organs.Mature lymphocyte migrate to peripheral lymphoid organsRecirculation of lymphocytesLymphocyte migrate to the sites of inflammation 5. Participate in regulation of apoptosisIntegrins inhibit cell apoptosisPart Clinical application of CD and adhesion molecule