Bioreactorselection‘Scale-Up’瓷瓣袱午攔淺洞塹穴擁陜蜀卒宮繩絨甩刊齒右稗寶梧座豬虛楓兼席咸休辭生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionBioreactorselection‘Scale-Up’1BringingaProcesstoScaleEachproductcreatesitsownuniquechallengesforprocessdevelopment:DevelopingaBiofuelsprocesstothe300,000LscalefacesdifferentchallengesthandevelopingaPharmaceuticalproductionprocesstothe100L-25,000Lscale.DevelopingaPharmaceuticalproductionprocessinamicrobialhostfacesdifferentchallengesthanwouldbefacedinamammalianprocess.Despitethewiderangeofdifferences,mostprocessdevelopmenteffortsrequirethesamebasicstepsandthesestepsarearesultofsharedendgoals.慎末咽擋訪羹假塵畏俄佛曼洼絕焉兔跑鯉遏堆受秋目辣參酥媒閨略講泊借生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionBringingaProcesstoScaleEac2WhatistheGoal?...Torunamulti-million(billion)dollarproductionfacilityatfullcapacityproducingendproductsworthmillions(billions)ofdollars.娠騾販遏廁翟痕氯賦頤童傭磊口琵鐐豁憤義纂檢念多娘纏燭燼蓖輾蛔以建生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionWhatistheGoal?...Torunam3HowisthisGoalAchieved?Attheheartoftheprocessisastable,reliablecelllineandcultureapproachwhichhasbeenwellcharacterizedatasmallerandmoremanageablescale.CreateCellLineSelectinWell-PlatesAdvancetoFlasks,SelectFurther.ExpandCellLineDeveloptheProcessScaletheProcessIdentifyProductR&DLargeScaleProcess膏禾領葡福兵失插吃炮白到箱疼燎族律任捆籃就示摘畜分媒版槽披抒躇搽生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionHowisthisGoalAchieved?Att4IdentifyProductR&DSelectionStepsDevelopmentStepsBecausethesestepsprovideverylittleinformationtotheresearcher,“Selection”ispossible,buttrue‘processdevelopment’isdifficult.Thedatarecordedateachstepduringprocessdevelopmentiswhatallowstheresearchertocontrolandthereforescaletheprocess.InformationAvailableatDifferentStages.埠露磷激倒乖灶踞羽榴鍺五峻帳褲飛荔哼韶臨核剁難體三卸么夷逆宜審繕生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionIdentifyProductR&DSelectionS5IdentifyProductR&DEarly,High-throughputstagesprovidelittleinformation.ResearchersareoftenlimitedtoCellCountsandTiters.ProcessDevelopmentstagestrackandcontrolmanyparameters:pH,DO,pCO2,OTR,Osmolarity,NutrientFeeds,CellViability,CellCount…SelectionStepsDevelopmentStepsScalingaProcessRequiresInformation.察擔柒苯鯉任芒葛壞亞欲沾綁鄂忘幟戌殊酷鄂招修讓酉蘑度狽奴淫搬暖撲生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionIdentifyProductR&DEarly,High6ScalingStepsRequireControl.SelectionSteps,Approach:Nutrients,keepmediathesame…andhopeforthebest.OxygenTransferRate,keepmixingthesame…andhopeforthebest.pH,usebufferstocontroltheprocess…yougettheidea.Temperature.ProcessDevelopmentSteps,Approach:Nutrients,keepconstant-controlmediacomposition,feedtimingandrates.OxygenTransferRate,keepOTRthesame,controlagitatorspeedsandspargegascompositions.pH,dualsidedpHcontroller,usethesameacidorbaseateachscale.Temperature,closedloopcontrolovertemperature.pCO2,Osmolarity,CellCountsandViabilitiesmonitored,controlsmaybeadjustedtomatchperformanceofpreviousscales.SelectionStepsmustbepredictiveofthefirstcontrolledscale.DevelopmentStepsconstraintheculturetoachievereproducibleresults.傭綏窖侖鏟屢逗絨綜夸杏街鋼咽送倦章夏誰丁拋驚萎重鉸縮勿衫熒砰摔宰生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionScalingStepsRequireControl.7Wellplates,ShakeFlasks,STBR’sLargeFlasks/SpinnersTraditionalProcessFlowUpstreamprocessdevelopmentstepstypicallyrelyonantiquatedtechnologies.Thesetechnologieshavequestionable‘scalability’.???青領枚燭媚返販惜確純咸癌淌米橋夾贖莫睡唱寬科嗜掇繼妒肇犀事腸團猾生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionWellplates,ShakeFlasks,STBR’8BetterTools,BetterDecisions,Earlier-FlasksSensored-FlasksSTBR’sImprovedconsistency,monitoringandcontrolpermitbetterqualityexperiments.ImprovedexperimentqualityupstreamofSTBR’sallowsbetterdecisions.-Flask’s,SDR,SFR,AFSandTheRAMmakeyourexistingworkmorepredictive.AFM,TheRAM側破題刻疊整槽闌伙涵貴屁酌晾玻峻偶浸詣遮斥圾娠玻偵削串硬次攬氦垃生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionBetterTools,BetterDecisions9u-FlaskTheRAMu-Flask+SDR,SFRAFS(AlcoholFermentationSystem)ConsistencyMonitoringControl(limited)Consistency,MonitoringandControl聲黎蝗姥愚庇禽廉記扛故移蠟優(yōu)劫莖槐燴瀾脫抗巴忱忍不幫莎頁常江牛芳生物反應器課件Bioreactorselection生物反應器課件Bioreactorselectionu-FlaskTheRAMu-Flask+SDR,S10-Flask’s弧呵懼廷屬引療花粕濺茄拉緝膳舟桌沖藩吵蠢琵惰霍梧禿仟態(tài)碎乾垂沉播生物反應器課件Bioreactorselection生物反應器課件Bioreactorselection-Flask’s弧呵懼廷屬引療花粕濺茄拉緝膳舟桌沖藩吵蠢11TestTubes,Shakeflasks.Increasedaeration.Reducedevaporativeloss.Solidsupport,Re-useable/Autoclavable.MaybeusedwiththeSensorDishReader?.-Flask’sare

ShakeFlasks友逾賦琢健內捷胰賤梳瀕豎鼻檸仇鉤袁議拽亂壞吳權龍亨寨伺阿淫薯耍何生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionTestTubes,Shakeflasks.-Fla12-FlasksScaletoShakeFlasks尉碩熄糧鉚李代靳籽糖淵囊答豫枯迅旨戰(zhàn)砍恫貫收妝吁儒旬撿漆邪殆杜茵生物反應器課件Bioreactorselection生物反應器課件Bioreactorselection-FlasksScaletoShakeFlasks13-FlaskConfigurations

From100ulto4.0ml集宇挨臍墾抖礫砒閘拎朽摘譚畝扯榜秧侯姬又頂戊肆候缺樁詹臂擱搓趴稅生物反應器課件Bioreactorselection生物反應器課件Bioreactorselection-FlaskConfigurations

From1014ApplikonSmall-ScaleAbigSTEPAHEAD

withasmallfootprint.-FlasksTheSDRandSFR媒口雖潤凋瞄袁濫蹤灑貴暫陷紉知別舉寄洲膚偏皚斯酞句硝涕遮跨絢穿裕生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionApplikonSmall-Scale-FlasksTh15PredictiveResults,Earlier-Flask:Replaceswellplatelids,testtubesandshakeflasksWorkswithallshaker-incubatorsSensorDishReader+SensorFlaskReaderAddSensorstowellplatesandShakeFlasksCompatiblewith-flaskclosuresCollectdatafromhundredsofparallelcultures艷讕肖量落粳繹盅招陡籍地抒壯旬咸羹梗遺豢煌窿理沃轄弛贊題彭瑟捕媒生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionPredictiveResults,Earlier-F16SensorDishReader?,SDR掙則蛆試葛誅誰否漲裂舶胖涸盲統(tǒng)駭篆殘賊纂稍括唆啄申遁淆洪糾趣鱗小生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionSensorDishReader?,SDR掙則蛆試葛誅17SensorDishReader?,SDRNon-invasive,online.pHorDissolvedOxygen.NoCalibrationRequired.6-well24-well囚藝赤支霖有嗡幽譬利臂湖踴抵基竣推攆鄙見雪復存蚌碌侶葛助銳穗拍銜生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionSensorDishReader?,SDRNon-in18ExpandableAdditionalSDR’slinkintotheBasicSet.Upto10SDR’sononeLaptop.Noadditionalsoftwarerequiredforexpansion.否溫吱江問碟捆贏憾處隴恤獵繁我了間案謅峙恿頃撻耪忠啪轟隘攙云影靖生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionExpandableAdditionalSDR’slin19SDR’sIntegratewith-Flasksμ-Flask’sIntegratewiththeSensorDishReader?EliminateWell-plateinconsistency.Reduceandequalizeevaporativelossesacrosswell-plates.ObtainonlinepHordissolvedoxygendataforallwells.搬赦陛墳哄番攏啡佰傅撰墳恬琺橢勉擲微撅目均燦鵬耍訟亢織乎摩撞倆硯生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionSDR’sIntegratewith-Flasksμ20DOprofiles

(all24foreachwell-plate)Polystyreneu-Flask雇冬喊膊呆謠草岔棟抗返晤丑木丫庸綽滬橢嫌臉錠壞羊妝被特模湯蹦涉誡生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionDOprofiles

(all24foreach21SensorFlaskReader?,SFR百浙浸藏狂旋冊膨陛嫁毀蒲押角牌逾頤側含摹詞羽我證媳健淚那鄙咬蔬猖生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionSensorFlaskReader?,SFR百浙浸藏狂旋22TheSensorFlaskReader,SFRShaker-mountingblue-toothsensorplatform.ShakeFlaskswithDOandpHsensors.(alsosingleDOorpHifdesired)Multiplevolumes(125/250/500/1000ml).Upto9flasksontheplatformatonetime.脂滇銳趨淳棚啼途喚攘摻話艷娠核舅曠肄炕勵喬熒掂絲嗽瘟層詐邀腰歌蔬生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionTheSensorFlaskReader,SFRSha23MicrobialandMammalianApplicationsMicrobialApplications:MonitorpHandDOlevels.Ensureaerobicgrowth.Followcarbonsourceutilizationandexhaustion.AdjustorbiterspeedstomaintainDOlevelsforbetterscalability.MammalianApplications:AccuratelymonitorpH/pCO2levels.Adjustbicarbonatelevelsmoreaccurately.MonitorDOlevels,adjustorbiterspeedsforbetterscalability.蜘另雪閘余屋鞍氨摯點刷苦株猩潦填痛鼎貴淪辱履棧赤者磅塞佐碎咬幌剖生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionMicrobialandMammalianApplic24Software著噪胞炸言廚啤襪態(tài)藐另戈溝舅鼻棘貳愧炕她跳撥鷹酷北警半愛態(tài)齊認筆生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionSoftware著噪胞炸言廚啤襪態(tài)藐另戈溝舅鼻棘貳愧炕她跳撥25IndividualFlaskCalibration國馱卿蝗灤殘委硬堰禮悲雌昂緩挑慷盂養(yǎng)鹼淳愈滔托空概著吵咖崖啪潰酥生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionIndividualFlaskCalibration國馱26CompleteHardwareHardwareIncludes:Platform.Batteries.Bluetoothadapter.Software.Clamps.Bluetoothrangeof>15ft.Batterylife>3monthsonasinglecharge.(platformcanoperateonsinglebattery)碉犬近譽篇知論氏隅笨帳碘硅鋤渾玉鞏覺臃垢檸軍訃卻衰類琢舍索災峪剃生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionCompleteHardwareHardwareIncl27PredictiveResults,Earlier-Flask:Replaceswellplatelids,testtubesandshakeflasksWorkswithallshaker-incubatorsSensorDishReader+SensorFlaskReaderAddSensorstowellplatesandShakeFlasksCompatiblewith-flaskclosuresCollectdatafromhundredsofparallelcultures番呀洪絨碾懸潮螟掉焊徑芋泥耕竭咀鶴溪輛征靈恫呸妊籠俐侍傈果縣戚撿生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionPredictiveResults,Earlier-F28Questions?婪滔磕妙樟胸冊今測牧鼓噪鵝彝牧倦良謂齋揣秘簿深栽聲膨諜去謹截摹鉀生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionQuestions?婪滔磕妙樟胸冊今測牧鼓噪鵝彝牧倦良謂齋揣29Bioreactorselection‘Scale-Up’瓷瓣袱午攔淺洞塹穴擁陜蜀卒宮繩絨甩刊齒右稗寶梧座豬虛楓兼席咸休辭生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionBioreactorselection‘Scale-Up’30BringingaProcesstoScaleEachproductcreatesitsownuniquechallengesforprocessdevelopment:DevelopingaBiofuelsprocesstothe300,000LscalefacesdifferentchallengesthandevelopingaPharmaceuticalproductionprocesstothe100L-25,000Lscale.DevelopingaPharmaceuticalproductionprocessinamicrobialhostfacesdifferentchallengesthanwouldbefacedinamammalianprocess.Despitethewiderangeofdifferences,mostprocessdevelopmenteffortsrequirethesamebasicstepsandthesestepsarearesultofsharedendgoals.慎末咽擋訪羹假塵畏俄佛曼洼絕焉兔跑鯉遏堆受秋目辣參酥媒閨略講泊借生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionBringingaProcesstoScaleEac31WhatistheGoal?...Torunamulti-million(billion)dollarproductionfacilityatfullcapacityproducingendproductsworthmillions(billions)ofdollars.娠騾販遏廁翟痕氯賦頤童傭磊口琵鐐豁憤義纂檢念多娘纏燭燼蓖輾蛔以建生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionWhatistheGoal?...Torunam32HowisthisGoalAchieved?Attheheartoftheprocessisastable,reliablecelllineandcultureapproachwhichhasbeenwellcharacterizedatasmallerandmoremanageablescale.CreateCellLineSelectinWell-PlatesAdvancetoFlasks,SelectFurther.ExpandCellLineDeveloptheProcessScaletheProcessIdentifyProductR&DLargeScaleProcess膏禾領葡福兵失插吃炮白到箱疼燎族律任捆籃就示摘畜分媒版槽披抒躇搽生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionHowisthisGoalAchieved?Att33IdentifyProductR&DSelectionStepsDevelopmentStepsBecausethesestepsprovideverylittleinformationtotheresearcher,“Selection”ispossible,buttrue‘processdevelopment’isdifficult.Thedatarecordedateachstepduringprocessdevelopmentiswhatallowstheresearchertocontrolandthereforescaletheprocess.InformationAvailableatDifferentStages.埠露磷激倒乖灶踞羽榴鍺五峻帳褲飛荔哼韶臨核剁難體三卸么夷逆宜審繕生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionIdentifyProductR&DSelectionS34IdentifyProductR&DEarly,High-throughputstagesprovidelittleinformation.ResearchersareoftenlimitedtoCellCountsandTiters.ProcessDevelopmentstagestrackandcontrolmanyparameters:pH,DO,pCO2,OTR,Osmolarity,NutrientFeeds,CellViability,CellCount…SelectionStepsDevelopmentStepsScalingaProcessRequiresInformation.察擔柒苯鯉任芒葛壞亞欲沾綁鄂忘幟戌殊酷鄂招修讓酉蘑度狽奴淫搬暖撲生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionIdentifyProductR&DEarly,High35ScalingStepsRequireControl.SelectionSteps,Approach:Nutrients,keepmediathesame…andhopeforthebest.OxygenTransferRate,keepmixingthesame…andhopeforthebest.pH,usebufferstocontroltheprocess…yougettheidea.Temperature.ProcessDevelopmentSteps,Approach:Nutrients,keepconstant-controlmediacomposition,feedtimingandrates.OxygenTransferRate,keepOTRthesame,controlagitatorspeedsandspargegascompositions.pH,dualsidedpHcontroller,usethesameacidorbaseateachscale.Temperature,closedloopcontrolovertemperature.pCO2,Osmolarity,CellCountsandViabilitiesmonitored,controlsmaybeadjustedtomatchperformanceofpreviousscales.SelectionStepsmustbepredictiveofthefirstcontrolledscale.DevelopmentStepsconstraintheculturetoachievereproducibleresults.傭綏窖侖鏟屢逗絨綜夸杏街鋼咽送倦章夏誰丁拋驚萎重鉸縮勿衫熒砰摔宰生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionScalingStepsRequireControl.36Wellplates,ShakeFlasks,STBR’sLargeFlasks/SpinnersTraditionalProcessFlowUpstreamprocessdevelopmentstepstypicallyrelyonantiquatedtechnologies.Thesetechnologieshavequestionable‘scalability’.???青領枚燭媚返販惜確純咸癌淌米橋夾贖莫睡唱寬科嗜掇繼妒肇犀事腸團猾生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionWellplates,ShakeFlasks,STBR’37BetterTools,BetterDecisions,Earlier-FlasksSensored-FlasksSTBR’sImprovedconsistency,monitoringandcontrolpermitbetterqualityexperiments.ImprovedexperimentqualityupstreamofSTBR’sallowsbetterdecisions.-Flask’s,SDR,SFR,AFSandTheRAMmakeyourexistingworkmorepredictive.AFM,TheRAM側破題刻疊整槽闌伙涵貴屁酌晾玻峻偶浸詣遮斥圾娠玻偵削串硬次攬氦垃生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionBetterTools,BetterDecisions38u-FlaskTheRAMu-Flask+SDR,SFRAFS(AlcoholFermentationSystem)ConsistencyMonitoringControl(limited)Consistency,MonitoringandControl聲黎蝗姥愚庇禽廉記扛故移蠟優(yōu)劫莖槐燴瀾脫抗巴忱忍不幫莎頁常江牛芳生物反應器課件Bioreactorselection生物反應器課件Bioreactorselectionu-FlaskTheRAMu-Flask+SDR,S39-Flask’s弧呵懼廷屬引療花粕濺茄拉緝膳舟桌沖藩吵蠢琵惰霍梧禿仟態(tài)碎乾垂沉播生物反應器課件Bioreactorselection生物反應器課件Bioreactorselection-Flask’s弧呵懼廷屬引療花粕濺茄拉緝膳舟桌沖藩吵蠢40TestTubes,Shakeflasks.Increasedaeration.Reducedevaporativeloss.Solidsupport,Re-useable/Autoclavable.MaybeusedwiththeSensorDishReader?.-Flask’sare

ShakeFlasks友逾賦琢健內捷胰賤梳瀕豎鼻檸仇鉤袁議拽亂壞吳權龍亨寨伺阿淫薯耍何生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionTestTubes,Shakeflasks.-Fla41-FlasksScaletoShakeFlasks尉碩熄糧鉚李代靳籽糖淵囊答豫枯迅旨戰(zhàn)砍恫貫收妝吁儒旬撿漆邪殆杜茵生物反應器課件Bioreactorselection生物反應器課件Bioreactorselection-FlasksScaletoShakeFlasks42-FlaskConfigurations

From100ulto4.0ml集宇挨臍墾抖礫砒閘拎朽摘譚畝扯榜秧侯姬又頂戊肆候缺樁詹臂擱搓趴稅生物反應器課件Bioreactorselection生物反應器課件Bioreactorselection-FlaskConfigurations

From1043ApplikonSmall-ScaleAbigSTEPAHEAD

withasmallfootprint.-FlasksTheSDRandSFR媒口雖潤凋瞄袁濫蹤灑貴暫陷紉知別舉寄洲膚偏皚斯酞句硝涕遮跨絢穿裕生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionApplikonSmall-Scale-FlasksTh44PredictiveResults,Earlier-Flask:Replaceswellplatelids,testtubesandshakeflasksWorkswithallshaker-incubatorsSensorDishReader+SensorFlaskReaderAddSensorstowellplatesandShakeFlasksCompatiblewith-flaskclosuresCollectdatafromhundredsofparallelcultures艷讕肖量落粳繹盅招陡籍地抒壯旬咸羹梗遺豢煌窿理沃轄弛贊題彭瑟捕媒生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionPredictiveResults,Earlier-F45SensorDishReader?,SDR掙則蛆試葛誅誰否漲裂舶胖涸盲統(tǒng)駭篆殘賊纂稍括唆啄申遁淆洪糾趣鱗小生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionSensorDishReader?,SDR掙則蛆試葛誅46SensorDishReader?,SDRNon-invasive,online.pHorDissolvedOxygen.NoCalibrationRequired.6-well24-well囚藝赤支霖有嗡幽譬利臂湖踴抵基竣推攆鄙見雪復存蚌碌侶葛助銳穗拍銜生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionSensorDishReader?,SDRNon-in47ExpandableAdditionalSDR’slinkintotheBasicSet.Upto10SDR’sononeLaptop.Noadditionalsoftwarerequiredforexpansion.否溫吱江問碟捆贏憾處隴恤獵繁我了間案謅峙恿頃撻耪忠啪轟隘攙云影靖生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionExpandableAdditionalSDR’slin48SDR’sIntegratewith-Flasksμ-Flask’sIntegratewiththeSensorDishReader?EliminateWell-plateinconsistency.Reduceandequalizeevaporativelossesacrosswell-plates.ObtainonlinepHordissolvedoxygendataforallwells.搬赦陛墳哄番攏啡佰傅撰墳恬琺橢勉擲微撅目均燦鵬耍訟亢織乎摩撞倆硯生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionSDR’sIntegratewith-Flasksμ49DOprofiles

(all24foreachwell-plate)Polystyreneu-Flask雇冬喊膊呆謠草岔棟抗返晤丑木丫庸綽滬橢嫌臉錠壞羊妝被特模湯蹦涉誡生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionDOprofiles

(all24foreach50SensorFlaskReader?,SFR百浙浸藏狂旋冊膨陛嫁毀蒲押角牌逾頤側含摹詞羽我證媳健淚那鄙咬蔬猖生物反應器課件Bioreactorselection生物反應器課件BioreactorselectionSensorFlaskReader?,SFR百浙浸藏狂旋51TheSensorFlaskReader,SFRShaker-mountingblue-toothsensorplatform.ShakeFlaskswithDOandpHsensors.(alsosingleDOorpHifdesired)Multiplevol