Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemENorartocarpetinCat.No.:HY-N10503CASNo.:520-30-9分?式:C??H??O?分?量:286.24儲存?式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY?物活性Norartocarpetin?種酪氨酸酶抑制劑。Norartocarpetin具有較強的酪氨酸酶抑制活性，其抑制活性IC50值為0.47μM。Norartocarpetin作為?種抗褐變劑可?于?品體系的研究。Norartocarpetin對肺癌細胞(NCI-H460)也具有顯著的抗癌活性，其IC50值為22μM。Norartocarpetin的抗增殖作?通過靶向Ras/Raf/MAPK信號通路、線粒體介導的細胞凋亡、S期細胞周期阻滯和抑制細胞遷移和侵襲實現(xiàn)的。IC50&TargetIC50:0.47μM(tyrosinase);[1]IC50:22μM(NCI-H460cells);85μM(MRC-9cells)[2]體外研究NorartocarpetinhasstrongmushroomtyrosinaseinhibitoryactivitywithanIC50valueof0.47μM[1].Norartocarpetin(0-100μM;48h)ledstodose-dependentcytotoxiceffectsinNCI-H460cellsandMRC-9cellswithIC50valuesof22μMand85μM,respectively[2].Norartocarpetin(0,11,22,and44μM;24h)ledtoblockadeofRas/Raf/MAPKsignallingpathway[2].Norartocarpetin(0,11,22,and44μM,24h)inducedtheapoptosisofthehumanlungcarcinomacells(NCI-H460)[2].Norartocarpetin(0,11,22,and44μM)ledstoS-phasecellcyclearrest[2].Norartocarpetin(22μM;24h)ledstodose-dependentsuppressionofcellinvasion[2].Norartocarpetin(0,11,22,and44μM,24h)ledstosignificantinhibitionofcellmigration[2].CellViabilityAssay[2]CellLine:MRC-9(normalfibroblastlungcells)andNCI-H460(humanlungcancercells)Concentration:0,3.12,6.25,12.5,25,50,and100μM(firstdissolvedinDMSOat1.0mg/mLandthendilutedtodifferentconcentrationsusingDMSO,30μL,20min)1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEIncubationTime:48hResult:RepressedtheviabilityofNCI-H460cellsinadose-dependentmanner.WesternBlotAnalysis[2]CellLine:MRC-9andNCI-H460cellsConcentration:0,11,22,and44μMIncubationTime:24hResult:Significantlyreducedtheexpressionofp-RAS,p-RAFandp-P38andunchangedtheexpressionsofRAS,RAFandP38.ApoptosisAnalysis[2]CellLine:MRC-9andNCI-H460cellsConcentration:0.11,22and44μMIncubationTime:24hResult:Ledtodose-dependentapoptosisandinducedDNAdamage.CellCycleAnalysis[2]CellLine:MRC-9andNCI-H460cellsConcentration:0,11,22,and44μMIncubationTime:Result:ShowedincreaseofS-phasecellsandthepercentageofS-phaseNCI-H460LCcellswas30%,40%,55%and70%at0,11,22and44μMconcentrations,respectively.CellInvasionAssayMRC-9andNCI-H460cellsCellLine:MRC-9andNCI-H460cellsConcentration:22μMIncubationTime:24hResult:Significantlyreducted(from100%toabout40%)intheinvasionofNCI-H460humanLCcells.CellMigrationAssay[2]2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemECellLine:MRC-9andNCI-H460cellsConcentration:0,11,22,and44μMIncubationTime:24hResult:DecreasedremarkablythecellinvasioninNCI-H460LCcellsinaconcentration-dependentmanner.McePdfHeightCaution:Producthasnotbeenfullyvalidatedfor