Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEPCI-33380Cat.No.:HY-100335CASNo.:1022899-36-0分?式:C??H??BF?N??O?分?量:855.78作?靶點(diǎn):Btk作?通路:ProteinTyrosineKinase/RTK儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:≥50mg/mL(58.43mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM1.1685mL5.8426mL11.6852mL5mM0.2337mL1.1685mL2.3370mL10mM0.1169mL0.5843mL1.1685mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%saline1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESolubility:≥2.5mg/mL(2.92mM);Clearsolution2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(2.92mM);ClearsolutionBIOLOGICALACTIVITY?物活性PCI-33380布魯頓酪氨酸激酶(BTK)的選擇性的、不可逆抑制劑(熒光探針)。IC50&TargetBTK[1][2].體外研究PCI-33380boundtoBtkcouldbedetectedbydenaturinggelelectrophoresisandfluorescentgelscanning[2].Immunofluorescence[2]CellLine:HumanBcells.Concentration:2μM.IncubationTime:1h.Result:Foundthat10nMofPCI-32765wassufficienttofullyoccupytheactivesiteofBtkinprimaryBcellsinculturebyusingthefluorescentlytaggedderivativePCI-33380.REFERENCES[1].ZuoY,etal.Anovel2,5-diaminopyrimidine-basedaffinityprobeforBruton'styrosinekinase.SciRep.2015Nov4;5:16136.[2].HonigbergLA,etal.TheBrutontyrosinekinaseinhibitorPCI-32765blocksB-cellactivationandisefficaciousinmodelsofautoimmunediseaseandB-cellmalignancy.ProcNatlAcadSciUSA.2010Jul20;107(29):13075-80.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400