Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEPolydatinCat.No.:HY-N0120ACASNo.:27208-80-6Synonyms:Piceid分?式:C??H??O?分?量:390.38作?靶點:Autophagy;Apoptosis;Mitophagy作?通路:Autophagy;Apoptosis儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數據體外實驗DMSO:≥29mg/mL(74.29mM)H2O:0.1mg/mL(0.26mM;Needultrasonic)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制備儲備液1mM2.5616mL12.8080mL25.6161mL5mM0.5123mL2.5616mL5.1232mL10mM0.2562mL1.2808mL2.5616mL請根據產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內使?，-20°C儲存時，請在1個?內使?。體內實驗請根據您的實驗動物和給藥?式選擇適當的溶解?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結果的可靠性，澄的儲備液可以根據儲存條件，適當保存；體內實驗的?作液，建議您現?現配，當天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現沉淀1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE、析出現象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(6.40mM);Clearsolution2.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(6.40mM);Clearsolution3.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(6.40mM);ClearsolutionBIOLOGICALACTIVITY?物活性Polydatin提取?從傳統中藥虎杖根，在多個實驗模型中具有抗炎作?。Polydatin(Piceid)可抑制G6PD，并誘導氧化和內質?應激IC50&TargetNF-κB,Autophagy體外研究Polydatinprotectscerebralcellsfromischemicdamagesviaimprovementofmicrocirculationandinhibitionofplateletaggregation.Inaddition,polydatininhibitsICAM-1expressioninendothelialcellsstimulatedbylipopolysaccharide;italsoattenuatesadhesionbetweenwhitebloodcellsandendothelialcells[1].Polydatininhibitscancercellproliferationandcellcycleprogression[4].PolydatininducesERstress-drivencelldeath[4].體內研究PolydatincouldsignificantlyincreasetheactivityofSODandtheheartrate,attenuatemyocardialpathologicaldamage,decreaseMDAcontent,slightlyincreasearterialpressureandGSH-Pxactivity,reduceintervalsofQRS,QTandST,andlowerFFAcontent[2].PROTOCOLCellAssay[3]TheeffectofpolydatinonmMECviabilityisevaluatedwithanMTTassay.mMECsareincubatedinthepresenceorabsenceofvariousconcentrationsofpolydatin(25,50and100μg/mL)andDEX(100μg/mL)for24h.Next,20μLofMTT(5mg/mL)isaddedtoeachwellandincubatedfor4h.Afterthesupernatantsareremovedandtheformazanisdis?solvedwith150μLofDMSOineachwell,theopticaldensity(OD)valueismeasuredat570nmonamicroplatereader[3].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.AnimalRats:Ratsaredividedintosixgroupsbyrandomassignmentandtreatedasfollows:innormalgroupandAdministration[2][3]polydatincontrolgroup:ratsareadministratedwithCMC-Naandpolydatin(200μmol/kg)bygavagerespectively,andgivennormalsaline(NS)bytailintravenous(iv)injectionwiththesamevolume;inDOXgroup:ratsareinjectedwithDOXbycaudaveinfor4weeks(3mg/kgperweek),thecumulativedosageis12mg/kgsimilartothatintheresearchofChangetal[2].Mice:Polydatinisdissolvedindimethylsulfoxide(DMSO)anddilutedinDulbecco’smodifiedEagle’smedium(DMEM).SixtyadultfemalepostpartumandlactatingBALB/cmice(6–8weeksold,weighing35–40g)areobtained.Controlgroup(CG):Themicearetreatedwith100μLofPBSasavehiclecontrol.Polydatin2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEgroups:24hafterSaureusinfection,themousemodelofSaureusmastitisisintraperitoneallyadministeredpolydatinat15,30and45mg/kg[3].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產品發(fā)表的科研?獻?OxidMedCellLongev.2022Mar27;2022:5696686.?JEthnopharmacol.2021Jul31;114479.?NeurotoxRes.20August2022.?MolMedRep.2022Jan;25(1):9.?PlantDirect.2022Sep2;6(9):e442.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].ChengY,etal.Involvementofcelladhesionmoleculesinpolydatinprotectionofbraintissuesfromischemia-reperfusioninjury.BrainRes.2006Sep19;1110(1):193-200.[2].WangHL,etal.SynergisticeffectsofPolydatinandVitaminCinInhibitingCardiotoxicityinducedbyDoxorubicininrats.FundamClinPharmacol.2016Nov28.[Epubaheadofprint][3].JiangKF,etal.PolydatinamelioratesStaphylococcusaureus-inducedmastitisinmiceviainhibitingTLR2-mediatedactivationofthep38MAPK/NF-κBpathway.ActaPharmacolSin.2016Nov28.[Epubaheadofprint][4].LuigiMele,etal.ANewInhibitorofglucose-6-phosphateDehydrogenaseBlocksPentosePhosphatePathwayandSuppressesMalignantProliferationandMetastasisinVivo.Cell