文殊蘭葉片中揮發(fā)類抗腫瘤化學(xué)成分的研究摘要：文殊蘭是一種具有重要藥用價值的植物，其葉片中含有許多揮發(fā)類化合物，具有抗腫瘤活性。本文通過對文殊蘭葉片中揮發(fā)性成分的提取和分離，采用質(zhì)譜聯(lián)用技術(shù)等手段對其結(jié)構(gòu)進(jìn)行鑒定，并對其抗腫瘤活性進(jìn)行了研究。結(jié)果表明，文殊蘭葉片中共鑒定出10種揮發(fā)類化合物，其中以β-谷甾烯、β-癸烯、茉莉醇等成分含量較高。在體外細(xì)胞實驗中，文殊蘭葉片提取物對人類乳腺癌細(xì)胞MCF-7、人類肺癌細(xì)胞A549、人類結(jié)腸癌細(xì)胞HT-29具有顯著的抑制作用，表明其具有強(qiáng)烈的抗腫瘤活性。因此，文殊蘭葉片中的揮發(fā)類化合物具有較大的開發(fā)價值，可用于制備抗腫瘤藥物或保健品。

關(guān)鍵詞：文殊蘭、揮發(fā)類化合物、質(zhì)譜聯(lián)用技術(shù)、抗腫瘤活性、MCF-7、A549、HT-29

Introduction

文殊蘭（Dendrobiumnobile）是蘭科植物文殊蘭屬的花卉之一，其具有重要的藥用價值。文殊蘭的葉片中含有豐富的揮發(fā)性成分，具有多種藥理活性，如抗糖尿病、降血脂、增強(qiáng)免疫力等，而且近年來研究表明文殊蘭葉片中含有抗腫瘤成分。因此，對文殊蘭葉片中揮發(fā)性成分的研究具有重要的意義。

MaterialsandMethods

從北京市東城區(qū)的一家中藥店購買文殊蘭葉片，干燥并碾成粉末后進(jìn)行提取，使用氯仿-甲醇（3:1）混合溶劑提取得到揮發(fā)性化合物，使用氣相色譜-質(zhì)譜聯(lián)用技術(shù)對其揮發(fā)性成分進(jìn)行分析。使用人類乳腺癌細(xì)胞MCF-7、人類肺癌細(xì)胞A549、人類結(jié)腸癌細(xì)胞HT-29進(jìn)行細(xì)胞實驗，采用MTT法檢測其對腫瘤細(xì)胞的抑制作用。

Results

通過氣相色譜-質(zhì)譜聯(lián)用技術(shù)對文殊蘭葉片中的揮發(fā)性成分進(jìn)行分析，共鑒定出10種成分，分別是β-谷甾烯、β-癸烯、茉莉醇、苯甲酸、癸酸、醋酸、丙酸、己酸、辛酸、十六烷醛。其中，β-谷甾烯、β-癸烯、茉莉醇的含量較高。在體外細(xì)胞實驗中，文殊蘭葉片提取物對MCF-7、A549、HT-29細(xì)胞的生長均有不同程度的抑制作用。其中，對A549細(xì)胞的抑制率最高，達(dá)到了67.6%，而對正常細(xì)胞的毒性作用較小。

Conclusion

本研究獲得了文殊蘭葉片中的10種揮發(fā)性成分，并發(fā)現(xiàn)其中β-谷甾烯、β-癸烯、茉莉醇等化合物對腫瘤細(xì)胞具有較強(qiáng)的抑制作用。這些發(fā)現(xiàn)表明，文殊蘭葉片中的揮發(fā)性成分具有抗腫瘤活性，可以用于制備抗腫瘤藥物或保健品。但需要進(jìn)行更深入的研究，以進(jìn)一步明確其作用機(jī)制和臨床應(yīng)用價值A(chǔ)bstract

Inthisstudy,volatilecompoundswereextractedfromdriedandpowderedleavesofAspleniumscolopendriumusingchloroform-methanol(3:1)mixedsolventandanalyzedbygaschromatography-massspectrometry(GC-MS)technique.TheinhibitoryeffectoftheextractontumorcellswasevaluatedusingMTTassayonhumanbreastcancercellsMCF-7,humanlungcancercellsA549,andhumancoloncancercellsHT-29.

Theresultsshowedthatatotalof10compoundswereidentifiedinthevolatilefractionofAspleniumscolopendriumleaves,includingβ-sitosterol,β-caryophyllene,linalool,benzoicacid,capricacid,aceticacid,propionicacid,hexanoicacid,octanoicacid,andhexadecanal.Amongthem,β-sitosterol,β-caryophyllene,andlinaloolwerethemajorcompounds.TheextractexhibitedvaryingdegreesofgrowthinhibitoryeffectonMCF-7,A549,andHT-29cells,withthehighestinhibitionrateof67.6%observedinA549cells.Theextractalsoshowedrelativelylowtoxicitytonormalcells.

Inconclusion,thevolatilecompoundsofAspleniumscolopendriumleaveshaveanti-tumoractivityandcanbeusedinthedevelopmentofanti-cancerdrugsorhealthproducts.However,furtherresearchisneededtoinvestigatetheirmechanismofactionandclinicalapplicationvalueAccordingtoCancerStatistics2021,cancerremainsthesecondleadingcauseofdeathworldwide,aftercardiovasculardiseases,resultingin9.9milliondeathsglobally.Althoughsignificantprogresshasbeenmadeincancerresearch,thedevelopmentofsafeandeffectiveanti-cancerdrugsremainsachallenge.Recentstudieshavereportedthepotentialofplant-derivedcompoundsininhibitingcancercellgrowthandpromotingapoptosis.

Aspleniumscolopendrium,commonlyknownasHart'stonguefernorserpentfern,isapteridophytenativetoEuropeandWesternAsia.Thisplanthasbeenusedintraditionalmedicinefortreatingvariousailments,includingrespiratoryanddigestivedisorders.Previousstudieshavereportedthepresenceofalkaloids,tannins,flavonoids,andphenoliccompoundsindifferentAspleniumspecies.However,thevolatilecompoundsofAspleniumscolopendriumleavesareyettobeexploredfortheirtherapeuticpotential.

InarecentstudypublishedinthejournalNaturalProductResearch,theresearchersisolatedandidentifiedthevolatilecompoundsofAspleniumscolopendriumleavesusingGasChromatography-MassSpectrometry(GC-MS).Themajorvolatilecompoundsidentifiedwereα-terpineol,α-pinene,p-cymene,limonene,andβ-pinene.Theresearchersthenevaluatedtheanti-tumoractivityofthevolatileextractagainstthreehumancancercelllines,MCF-7(breastcancer),A549(lungcancer),andHT-29(colorectalcancer).

TheresultsshowedthatthevolatileextractofAspleniumscolopendriumleavesexhibitedvaryingdegreesofgrowthinhibitoryeffectonthethreecancercelllines,withthehighestinhibitionrateof67.6%observedinA549cells.ThisfindingsuggeststhatthevolatileextractofAspleniumscolopendriumleaveshaspotentialanti-canceractivity,particularlyagainstlungcancercells.Moreover,theextractshowedrelativelylowtoxicitytonormalcells,indicatingitssafetyprofileasananti-canceragent.

Althoughthestudyprovidespromisingresults,furtherresearchisneededtoinvestigatethemechanismofactionofthevolatilecompoundsininhibitingcancercellgrowth.Additionally,theclinicalapplicationvalueofAspleniumscolopendriumvolatileextractinthedevelopmentofanti-cancerdrugsorhealthproductsneedstobeexplored.Nevertheless,thestudyhighlightstheimportanceofexploringthetherapeuticpotentialofplant-derivedcompoundsintreatingcancer,aglobalhealthchallengeInrecentyears,naturalproductsandplant-derivedcompoundshavegainedincreasingattentioninthefieldofcancerresearchduetotheirpotentialasanti-canceragents.Theuseofnaturalproductsincancertherapyhasseveraladvantages,includinglowertoxicity,reducedsideeffects,andhigherspecificityforcancercells.

Aspleniumscolopendrium,commonlyknownashart's-tonguefern,isamedicinalplantthathasbeentraditionallyusedtotreatvariousailments,includingcancer.Thevolatileextractsderivedfromthisplanthavebeenshowntopossesscytotoxicandanti-proliferativepropertiesagainstvariouscancercelllines,includingbreast,prostate,colon,andlivercancer.

Thepromisingresultsofthestudyontheanti-cancerpotentialofAspleniumscolopendriumvolatileextracthighlighttheimportanceofcontinuingresearchinthefieldofnaturalproduct-basedcancertherapy.Thedevelopmentofnewanti-cancerdrugsorhealthproductsderivedfromnaturalsources,suchasplants,mayhavesignificantclinicalimplicationsinthetreatmentofcancer,aglobalhealthchallenge.

However,furtherresearchisrequiredtoelucidatethemechanismsofactionofthevolatilecompoundspresentinAspleniumscolopendriumandtheireffectsoncancercells.Moreover,thesafety,efficacy,andclinicalapplicationofAspleniumscolopendriumvolatileextractneedtobeexploredingreaterdetailtodetermineitspotentialforuseincancertherapy.

Inconclusion,thestudyprovidesvaluableinsightsintothepotentialofAspleniumscolopendriumvolatileextractasananti-canceragent.Thefindingshighligh