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M MChangePhysical MM MPhysical Isitpossiblethatyourtargetgeneisinthecloneasamarkerthatdoesnotco-segregatewithWhydoweneedalargesegregatingTonarrowdownyourtarget FineMapwhengenome/linkagemapavailable Increasepopulationsize, 分組混合分析法（Bulkedsegregantysis,+DNA/RNA-seq(二代基于性狀表現(xiàn)型的BSA根據(jù)目將或株系依據(jù)相對(duì)表型差異分成兩組，形成座所在的區(qū)域存在差異外，來自其他部分的DNA組成完因庫的一個(gè)隨BSA+RNA-SEQorDNA-CompleteSequencingof100BC1P1 --+++--+--+++-+++-- ……---++-++-+--- PoolingExtremeP1 ++++++++++++++++ … -------------------基于標(biāo)記型的BSA 依 型A1B1/A1B1和A2B2/A2B2構(gòu)建DNA混合池，它們是在目標(biāo)區(qū)段上存在差異，目標(biāo)區(qū)段以外的遺傳尋找兩DN混合之的型有在標(biāo)上與目標(biāo) 密鎖子記。PrincipleofBSA-RNA-ATATTTATTGWhiteGCCGATTGPurpelGTTTATTTGCCTACCGATTTATCGGTCGGCTTPaperstoTraditionalmarkers(anyreviewpaper,orMichelmoreRW,ParanI,KesseliRV.1991.Identificationofmarkerslinkedtodisease-genesbybulkedsegregantysis:arapidmethodtodetectmarkersinspecificgenomicregionsbyusingsegregatingpopulations.ProceedingsoftheNationalAcademyofSciences,USA88,9828–9832.BairdNA,etal.(2008)Ra