生化進(jìn)展課件第1頁，共19頁，2023年，2月20日，星期六Abstract1、Habronemamuscae(Spirurida:Habronematidae)occursinthestomachofequids,istransmittedbyadultmusciddipteransandcausesgastrichabronemiasis2、Scanningelectronmicroscopy(SEM)wasusedtostudythemorphologicalaspectsofadultwormsofthisnematodeindetail.第2頁，共19頁，2023年，2月20日，星期六INTRODUCTIONBackground：

1.Theadultwormslivesuperficially,underthemucouslayer,withonlytheirheadsburiedinthemucosalspacesinthestomach.2.ThemaineffectofHabronemainthestomachistostimulatethesecretionoflargeamountsofthick,stringy,tenaciousmucus,withassociatedhistologicalchangeconsistingofhyper-plasiaandhypertrophyofthemucus-secretingcells.Purpose：Althoughtherehavebeenstudiesevaluatingthemorphologyofavianhabronematidnematodesbasedonlightandscanningelectronmicroscopy,thepresent(SEM)studyisthefirsttodescribe,indetail,H.muscaeremovedfromanaturallyinfectedhorse.第3頁，共19頁，2023年，2月20日，星期六MATERIALSANDMETHODSMaterials:Atotalof36worms(22femalesand14males)wereremovedfromthestomachofa13-year-oldhorse.

第4頁，共19頁，2023年，2月20日，星期六Methods:1.

Thewormswerewashedin2%sodiumcacodylatebuffer(pH7.2),andthenclearedwithlactophenolsolution.2.

Identificationofthespecieswasconfirmedonthebasisoflightmicroscopicexamination.Amongexaminedworms,12wereidentifiedasH.muscae(sevenfemalesandfivemales).3.

Thenematodeswereputintofresh4%glutarldehydefor2h,postfixedin1%osmiumtetraoxideincacodylatebuffer(pH7.2)at4°Cfor1handdehydratedthroughaseriesofincreasingethanolconcentrations.第5頁，共19頁，2023年，2月20日，星期六4.

TheywerethendriedwithliquidCO2inacriticalpointdrierapparatus.5.

Eachwormwasplacedonanumberedaluminiumstab,whichwascoatedwithgoldinanioncoatingsputterandviewedwithaJEOLJSM-840ScanningElectronMicroscopeoperatedat15kV.PhotographsweretakenwithadigitalacquisitionsystemforanalogSEMs.第6頁，共19頁，2023年，2月20日，星期六RESULTSFig.1ScanningelectronmicrographoftheanteriorendofthefemaleHabronemamuscae,showingsub-mediancephalicpapilla(arrowhead),cervicalpapilla(CP),thelateralala(LA),andexcretorypore(EP).Bar=10μmFig.2ScanningelectronmicrographoftheanteriorendofthefemaleHabronemamuscae,showingcephalicregionwithtwolaterallips(Llip),eachwastrilobed(stars),foursub-mediancephalicpapillae(CP),andamphidalapertures(A).Bar=10μm第7頁，共19頁，2023年，2月20日，星期六Fig.3ScanningelectronmicrographoftheanteriorendofthefemaleHabronemamuscae,showingcephalicregionwithlaterallip(Llip),whichwastrilobed(stars),sub-mediancephalicpapillae(CP),andamphidalaperture(A).Bar=10μmFig.4ScanningelectronmicrographoftheanteriorendofthemaleHabronemamuscae,showingcervicalpapilla(CP).Bar=10μm第8頁，共19頁，2023年，2月20日，星期六Fig.5ScanningelectronmicrographoftheanteriorendofthemaleHabronemamuscae,showingexcretorypore(EP),andcuticulartransversestriations(TS).Bar=10μmFig.6ScanningelectronmicrographoftheanteriorendofthemaleHabronemamuscae,showingabnormalfeature,withlaterallipwhichwastrilobed(stars),sub-mediancephalicpapillae(CP)andamphidalapertures(A).Bar=10μm第9頁，共19頁，2023年，2月20日，星期六Fig.7ScanningelectronmicrographofthefemaleHabronemamuscae,showingvulva(V),andcuticulartransversestriations(TS).Bar=10μmFig.8ScanningelectronmicrographofthefemaleHabronemamuscae,showinganalpore(AP)andphasmid(Ph).Bar=100μm第10頁，共19頁，2023年，2月20日，星期六Fig.9ScanningelectronmicrographoftheposteriorendofthefemaleHabronemamuscae,showinganalpore(AP).Bar=10μmFig.10ScanningelectronmicrographoftheposteriorendofthefemaleHabronemamuscae,showingahighermagnificationofphasmids(Ph).Bar=10μm第11頁，共19頁，2023年，2月20日，星期六Fig.11ScanningelectronmicrographoftheposteriorendofthemaleHabronemamuscae,showingthecaudalalae(CA),spicule(S)andcuticularridges(CR).Bar=100μmFig.12ScanningelectronmicrographoftheposteriorendofthemaleHabronemamuscae,showingpre-cloacalpapillae(PrCP),spicule(S),postcloacalpapillae(PoCP),andcuticularridges(CR).Bar=100μm第12頁，共19頁，2023年，2月20日，星期六Fig.13ScanningelectronmicrographoftheposteriorendofthemaleHabronemamuscae,showingprecloacalpapillae(PrCP),andcuticularridges(CR).Bar=10μmFig.14ScanningelectronmicrographoftheposteriorendofthemaleHabronemamuscae,showingthecloaca(star),theunpairedpostcloacalpapillae,oneontherighthandside(RPoCP)andtwoonthelefthandside(LPoCP).Bar=10μm第13頁，共19頁，2023年，2月20日，星期六Fig.15ScanningelectronmicrographoftheposteriorendofthemaleHabronemamuscae,showingahighermagnificationoftheunpairedpostcloacalpapillae,oneontherighthandside(RPoCP)andtwoonthelefthandside(LPoCP),cuticularridges(CR),andcuticularbosses(CB).Bar=10μmFig.16ScanningelectronmicrographoftheposteriorendofthemaleHabronemamuscae,showingahighermagnificationoftheclusterofsmallpostcloacalpapillae(arrowheads),phasmids(Ph),andcuticularridges(CR).Bar=10μm第14頁，共19頁，2023年，2月20日，星期六DISCUSSIONThispaperdescribes,forthefirsttime,mostsurfacefeaturesofadultH.muscae(Spirurida,Habronematidae),removedfromnaturallyinfectedhorsebySEM.Another