原理度一抗體軍會(huì)物司蔓一3體復(fù)Valadononry.chAsposeslidesforNET4odientPEvaluationonly.CreatedwithAsposeSlidesforNET4.0dientProfilo71Copyright2004-2017AsposePtyL?放射免疫方法的建立原則根據(jù)分析目的和要求,通過(guò)對(duì)標(biāo)記物、抗體、標(biāo)準(zhǔn)品(校準(zhǔn)品)的合理匹配,以及對(duì)反應(yīng)條件、分離方法的選擇,達(dá)到最滿(mǎn)意的效果Valadononry.chAsposeslidesforNET4odientPEvaluationonly.CreatedwithAsposeSlidesforNET4.0dientProfilo71Copyright2004-2017AsposePtyL放射免疫設(shè)計(jì)的目標(biāo)根據(jù)要求使試劑的劑量反應(yīng)曲線(xiàn)在滿(mǎn)足靈敏度的情況下,是多數(shù)樣品得到較高的精密度Valadononry.chAsposeslidesforNET4odientPEvaluationonly.CreatedwithAsposeSlidesforNET4.0dientProfilo71Copyright2004-2017AsposePtyL目錄靈敏度的設(shè)計(jì)反應(yīng)條件的選擇反應(yīng)方式的選擇分離方法的選擇方法學(xué)鑒定Valadononry.chAsposeslidesforNET4odientPEvaluationonly.CreatedwithAsposeSlidesforNET4.0dientProfilo71Copyright2004-2017AsposePtyLFlylikeabutternycontento靈敏度的設(shè)計(jì)Valadononry.chAsposeslidesforNET4odientPEvaluationonly.CreatedwithAsposeSlidesforNET4.0dientProfilo71Copyright2004-2017AsposePtyL靈敏度的設(shè)計(jì)放射免疫分析的靈敏度Sensitivit指能測(cè)出的具有統(tǒng)計(jì)學(xué)意義的最小值,通常指“0”標(biāo)準(zhǔn)管結(jié)合率減兩個(gè)標(biāo)準(zhǔn)差的相應(yīng)劑量。Valadononry.chAsposeslidesforNET4odientPEvaluationonly.CreatedwithAsposeSlidesforNET4.0dientProfilo71Copyright2004-2017AsposePtyL靈敏度的設(shè)計(jì)放射免疫分析的靈敏度Sensitivity若以能測(cè)出的具有一定精密度的最小值為靈敏度的定義,則Figure2.Determineworkingrangeofassay.應(yīng)根據(jù)劑量反應(yīng)曲線(xiàn)的精密度圖(PDP),以CV%-10%的最小劑量為有效地可測(cè)量的最小劑量,即靈敏度。Valadononry.chAsposeslidesforNET4odientPEvaluationonly.CreatedwithAsposeSlidesforNET4.0dientProfilo71Copyright2004-2017AsposePtyL靈敏度的設(shè)計(jì)放射免疫分析的靈敏度ensitivity影響因素標(biāo)記物的比活性抗體的親和力和用量劑量反應(yīng)曲線(xiàn)濃度范圍Valadononry.chAsposeslidesforNET4odientPEvaluationonly.CreatedwithAsposeSlidesforNET4.0dientProfilo71Copyright2004-2017AsposePtyL標(biāo)記物的用量記物的用量∝計(jì)數(shù)率統(tǒng)計(jì)漲落計(jì)數(shù)誤差靈敏度樣品保持減少樣品定的計(jì)數(shù)率化學(xué)量①適當(dāng)比活性、穩(wěn)定的標(biāo)記物Valadononry.chAsposeslidesforNET4odientPEvaluationonly.CreatedwithAsposeSlidesforNET4.0dientProfilo71Copyright2004-2017AsposePtyL標(biāo)記物的用量最佳標(biāo)記抗原的選擇方法用不同濃度的標(biāo)記抗原作一系列的抗血清實(shí)驗(yàn),直到標(biāo)記物減少不再使曲線(xiàn)再右移,此時(shí)標(biāo)記物濃度稱(chēng)“減弱濃度”,即為加入標(biāo)記抗原化學(xué)量的最低濃度。Valadononry.chAsposeslidesforNET4odie