題目：MMRN1對PTMC遷移和增殖的研究

目錄摘要 MMRN1對PTMC遷移和增殖的研究摘要目的：探討MMRN1對PTMC（甲狀腺微小乳頭狀癌，屬于甲狀腺乳頭狀癌的一類，癌直徑≤10?mm）轉(zhuǎn)移和增殖作用的研究。方法：查詢PTMC轉(zhuǎn)移和增殖的差異性序列并篩選差異性基因；通過免疫組織化學(xué)，WesternBlot，qPCR等方法測定PTC細胞和正常細胞中MMRN1的表達情況；使用細胞劃痕實驗驗證MMRN1對PTC細胞遷徙的影響；利用細胞成團實驗判斷MMRN1對PTC細胞增殖的影響。結(jié)果：篩選到PTMC中顯著表達差異基因MMRN1；免疫組織化學(xué)，WesternBlot，qPCR等結(jié)果均顯示MMRN1在PTC細胞中高表達，且在BCPAP細胞中高表達于TPC-1細胞；WesternBlot結(jié)果顯示相比于正常甲狀腺細胞，BCPAP細胞中MMRN1的表達含量高64%，且相比于TPC-1細胞，BCPAP細胞中MMRN1的表達高39%；qPCR結(jié)果顯示相比于正常甲狀腺細胞，BCPAP細胞中MMRN1的表達含量高88%，且相比于TPC-1細胞，BCPAP細胞中MMRN1的表達高48%；細胞劃痕實驗結(jié)果顯示，PTC細胞遷徙能力強于正常甲狀腺細胞，且BCPAP細胞的遷徙能力強于TPC-1；細胞成團結(jié)果顯示，BCPAP成團聚集能力強于TPC-1,TPC-1成團能力強于正常甲狀腺細胞。結(jié)論：MMRN1與PTMC發(fā)生有關(guān)，并可能促使其遷移與增殖。關(guān)鍵詞MMRN1；甲狀腺癌細胞；增殖；遷移;

StudyonMMRN1invasionandproliferationofPTMCAbstractObject:ToinvestigatetheeffectofMMRN1onPTMC（papillarythyroidmicrocarcinoma,aclassofpapillarythyroidcarcinomawithacancerdiameterof≤10mm）invasionandproliferation.Methods:QuerythedifferentialsequencesofPTMCtransferandinvasionandscreenthedifferentialgenes;useimmunohistochemistry,WesternBlot,qPCRandothermethodswereusedtodetecttheexpressionofMMRN1inPTCcellsandnormalcells.CellscratchassaywasusedtodetecttheeffectofMMRN1onPTCcellmigration.CellclusteringassaywasusedtodetecttheeffectofMMRN1onPTCcellproliferation.Results:TherewasasignificantdifferenceexpressionofdifferentialgenesMMRN1inPTMC.Theresultsofimmunohistochemistry,WesternBlot,qPCRshowedthatMMRN1washighlyexpressedinPTCcellandhighlyexpressedinBCPAPcellsthanTCP-1cell;TheresultsofWesternBlotshowedthattheexpressionofMMRN1inBCPAPcellswashigherthanthatinnormalthyroidcellsabout64%,BCPAPcellsMMRN1expressionwashigherthanthatofTPC-1cellsabout39%;theresultsofqPCRshowedthatMMRN1expressioninBCPAPcellswashigherthanthatofnormalthyroidcells88%,TheexpressionofMMRN1inBCPAPcellswashigherthanthatofTPC-1cells48%;cellscratchtestresultsshowedthatPTCcellsmigratedstrongerthannormalthyroidcells,andBCPAPcellsmigratedstrongerthanTPC-1;cellclusteringresultsshowedthatPTCcellclusteringabilitywasstrongerthannormalthyroidcells.,andBCPAPclusteringabilitywasstrongerthanTPC-1.Conclusion:MMRN1maypromotetheinvasionandproliferationofPTMC.KeywordsMMRN1;PTMC;proliferation;invasion;

前言作為十分常見的內(nèi)分泌腫瘤，甲狀腺癌在全球范圍內(nèi)其發(fā)病率迅速增長，并成為近年來年增長率最快的實體惡性腫瘤。新發(fā)生的甲狀腺癌大多數(shù)都是甲狀腺乳頭狀癌（PTC）ADDINCITAVI.PLACEHOLDEReb28f11e-ea49-4324-9fbb-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[1]ADDINCITAVI.PLACEHOLDERfde350ad-aca3-4388-8270-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癌（PTMC）即腫瘤直徑≤10?mm。隨著其發(fā)病率和人們對其認識的不斷增長，現(xiàn)在甲狀腺彩超也列入了常規(guī)體檢部分。然而隨著影像學(xué)檢查的增多，甲狀腺微小乳頭狀癌（PTMC）的發(fā)病率在全球范圍內(nèi)迅猛增加ADDINCITAVI.PLACEHOLDER6c5eee83-3a44-406f-b88b-b2019805d928PFBsYWNlaG9sZGVyPg0KICA8QWRkSW5WZXJzaW9uPjUuNy4xLjA8L0FkZEluVmVyc2lvbj4NCiAgPElkPjZjNWVlZTgzLTNhNDQtNDA2Zi1iODhiLWIyMDE5ODA1ZDkyODwvSWQ+DQogIDxFbnRyaWVzPg0KICAgIDxFbnRyeT4NCiAgICAgIDxJZD5mMjZjZjk4My1iMmM3LTQ1NmYtOTJhMi02N2Y5MmRlMGIyYmM8L0lkPg0KICAgICAgPFJlZmVyZW5jZUlkPjExZTc4MGU0LTk3NTEtNDBlOC1hMDYwLWFmMGJiYTk4ZmE0YzwvUmVmZXJlbmNlSWQ+DQogICAgICA8UmFuZ2U+DQogICAgICAgIDxTdGFydD4yPC9TdGFydD4NCiAgICAgICAgPExlbmd0aD4zPC9MZW5ndGg+DQogICAgICA8L1JhbmdlPg0KICAgICAgPFJlZmVyZW5jZT4NCiAgICAgICAgPFJlZmVyZW5jZVR5cGVJZD5Kb3VybmFsQXJ0aWNsZTwvUmVmZXJlbmNlVHlwZUlkPg0KICAgICAgICA8QXV0aG9ycz4NCiAgICAgICAgICA8UGVyc29uPg0KICAgICAgICAgICAgPEZpcnN0TmFtZT5IeWVvbmc8L0ZpcnN0TmFtZT4NCiAgICAgICAgICAgIDxMYXN0TmFtZT5BaG48L0xhc3ROYW1lPg0KICAgICAgICAgICAgPE1pZGRsZU5hbWU+U2lrPC9NaWRkbGVOYW1lPg0KICAgICAgICAgIDwvUGVyc29uPg0KICAgICAgICAgIDxQZXJzb24+DQogICAgICAgICAgICA8Rmlyc3ROYW1lPkh5dW48L0ZpcnN0TmFtZT4NCiAgICAgICAgICAgIDxMYXN0TmFtZT5LaW08L0xhc3ROYW1lPg0KICAgICAgICAgICAgPE1pZGRsZU5hbWU+SnVuZzwvTWlkZGxlTmFtZT4NCiAgICAgICAgICAgIDxTZXg+RmVtYWxlPC9TZXg+DQogICAgICAgICAgPC9QZXJzb24+DQogICAgICAgICAgPFBlcnNvbj4NCiAgICAgICAgICAgIDxGaXJzdE5hbWU+SC48L0ZpcnN0TmFtZT4NCiAgICAgICAgICAgIDxMYXN0TmFtZT5XZWxjaDwvTGFzdE5hbWU+DQogICAgICAgICAgICA8TWlkZGxlTmFtZT5HaWxiZXJ0PC9NaWRkbGVOYW1lPg0KICAgICAgICAgIDwvUGVyc29uPg0KICAgICAgICA8L0F1dGhvcnM+DQogICAgICAgIDxEb2k+MTAuMTA1Ni9ORUpNcDE0MDk4NDE8L0RvaT4NCiAgICAgICAgPElkPjExZTc4MGU0LTk3NTEtNDBlOC1hMDYwLWFmMGJiYTk4ZmE0YzwvSWQ+DQogICAgICAgIDxMYW5ndWFnZT5lbmc8L0xhbmd1YWdlPg0KICAgICAgICA8TGFuZ3VhZ2VDb2RlPmVuPC9MYW5ndWFnZUNvZGU+DQogICAgICAgIDxMb2NhdGlvbnM+DQogICAgICAgICAgPExvY2F0aW9uPg0KICAgICAgICAgICAgPEFkZHJlc3M+MjUzNzIwODQ8L0FkZHJlc3M+DQogICAgICAgICAgICA8TG9jYXRpb25UeXBlPkVsZWN0cm9uaWNBZGRyZXNzPC9Mb2NhdGlvblR5cGU+DQogICAgICAgICAgPC9Mb2NhdGlvbj4NCiAgICAgICAgICA8TG9jYXRpb24+DQogICAgICAgICAgICA8QWRkcmVzcz4xMC4xMDU2L05FSk1wMTQwOTg0MTwvQWRkcmVzcz4NCiAgICAgICAgICAgIDxMb2NhdGlvblR5cGU+RWxlY3Ryb25pY0FkZHJlc3M8L0xvY2F0aW9uVHlwZT4NCiAgICAgICAgICA8L0xvY2F0aW9uPg0KICAgICAgICA8L0xvY2F0aW9ucz4NCiAgICAgICAgPE5vdGVzPkpvdXJuYWwgQXJ0aWNsZTwvTm90ZXM+DQogICAgICAgIDxOdW1iZXI+MTk8L051bWJlcj4NCiAgICAgICAgPFBhZ2VSYW5nZT48IVtDREFUQVs8c3A+DQogIDxuPjE3NjU8L24+DQogIDxpbj50cnVlPC9pbj4NCiAgPG9zPjE3NjU8L29zPg0KICA8cHM+MTc2NTwvcHM+DQo8L3NwPg0KPGVwPg0KICA8bj4xNzY3PC9uPg0KICA8aW4+dHJ1ZTwvaW4+DQogIDxvcz4xNzY3PC9vcz4NCiAgPHBzPjE3Njc8L3BzPg0KPC9lcD4NCjxvcz4xNzY1LTc8L29zPl1dPjwvUGFnZVJhbmdlPg0KICAgICAgICA8RW5kUGFnZT4xNzY3PC9FbmRQYWdlPg0KICAgICAgICA8U3RhcnRQYWdlPjE3NjU8L1N0YXJ0UGFnZT4NCiAgICAgICAgPFBlcmlvZGljYWw+DQogICAgICAgICAgPE5hbWU+VGhlIE5ldyBFbmdsYW5kIGpvdXJuYWwgb2YgbWVkaWNpbmU8L05hbWU+DQogICAgICAgICAgPFVzZXJBYmJyZXZpYXRpb24xPk4gRW5nbCBKIE1lZDwvVXNlckFiYnJldmlhdGlvbjE+DQogICAgICAgIDwvUGVyaW9kaWNhbD4NCiAgICAgICAgPFB1Yk1lZElkPjI1MzcyMDg0PC9QdWJNZWRJZD4NCiAgICAgICAgPFNlcXVlbmNlTnVtYmVyPjI8L1NlcXVlbmNlTnVtYmVyPg0KICAgICAgICA8U2hvcnRUaXRsZT5BaG4sIEtpbSBldCBhbC4gMjAxNCDigJMgS29yZWEncyB0aHlyb2lkLWNhbmNlciBlcGlkZW1pYy0tc2NyZWVuaW5nIGFuZCBvdmVyZGlhZ25vc2lzPC9TaG9ydFRpdGxlPg0KICAgICAgICA8U291cmNlT2ZCaWJsaW9ncmFwaGljSW5mb3JtYXRpb24+UHViTWVkPC9Tb3VyY2VPZkJpYmxpb2dyYXBoaWNJbmZvcm1hdGlvbj4NCiAgICAgICAgPFRpdGxlPktvcmVhJ3MgdGh5cm9pZC1jYW5jZXIgImVwaWRlbWljIi0tc2NyZWVuaW5nIGFuZCBvdmVyZGlhZ25vc2lzPC9UaXRsZT4NCiAgICAgICAgPFZvbHVtZT4zNzE8L1ZvbHVtZT4NCiAgICAgICAgPFllYXI+MjAxNDwvWWVhcj4NCiAgICAgIDwvUmVmZXJlbmNlPg0KICAgIDwvRW50cnk+DQogICAgPEVudHJ5Pg0KICAgICAgPElkPjUzN2I5ZmZmLTg0MWMtNGU3Yy1hZmUzLWUwYTE2OWY3N2Q3NzwvSWQ+DQogICAgICA8UmVmZXJlbmNlSWQ+NmQ2MmMwYTMtNWYwMy00MGE1LWJlNjAtMmJlODZlYmVhYjA1PC9SZWZlcmVuY2VJZD4NCiAgICAgIDxSYW5nZT4NCiAgICAgICAgPFN0YXJ0PjA8L1N0YXJ0Pg0KICAgICAgICA8TGVuZ3RoPjI8L0xlbmd0aD4NCiAgICAgIDwvUmFuZ2U+DQogICAgICA8UmVmZXJlbmNlPg0KICAgICAgICA8UmVmZXJlbmNlVHlwZUlkPkpvdXJuYWxBcnRpY2xlPC9SZWZlcmVuY2VUeXBlSWQ+DQogICAgICAgIDxBdXRob3JzPg0KICAgICAgICAgIDxQZXJzb24+DQogICAgICAgICAgICA8Rmlyc3ROYW1lPkxvdWlzZTwvRmlyc3ROYW1lPg0KICAgICAgICAgICAgPExhc3ROYW1lPkRhdmllczwvTGFzdE5hbWU+DQogICAgICAgICAgICA8U2V4PkZlbWFsZTwvU2V4Pg0KICAgICAgICAgIDwvUGVyc29uPg0KICAgICAgICAgIDxQZXJzb24+DQogICAgICAgICAgICA8Rmlyc3ROYW1lPkguPC9GaXJzdE5hbWU+DQogICAgICAgICAgICA8TGFzdE5hbWU+V2VsY2g8L0xhc3ROYW1lPg0KICAgICAgICAgICAgPE1pZGRsZU5hbWU+R2lsYmVydDwvTWlkZGxlTmFtZT4NCiAgICAgICAgICA8L1BlcnNvbj4NCiAgICAgICAgPC9BdXRob3JzPg0KICAgICAgICA8RG9pPjEwLjEwMDEvamFtYW90by4yMDE0LjE8L0RvaT4NCiAgICAgICAgPElkPjZkNjJjMGEzLTVmMDMtNDBhNS1iZTYwLTJiZTg2ZWJlYWIwNTwvSWQ+DQogICAgICAgIDxMYW5ndWFnZT5lbmc8L0xhbmd1YWdlPg0KICAgICAgICA8TGFuZ3VhZ2VDb2RlPmVuPC9MYW5ndWFnZUNvZGU+DQogICAgICAgIDxMb2NhdGlvbnM+DQogICAgICAgICAgPExvY2F0aW9uPg0KICAgICAgICAgICAgPEFkZHJlc3M+MjQ1NTc1NjY8L0FkZHJlc3M+DQogICAgICAgICAgICA8TG9jYXRpb25UeXBlPkVsZWN0cm9uaWNBZGRyZXNzPC9Mb2NhdGlvblR5cGU+DQogICAgICAgICAgPC9Mb2NhdGlvbj4NCiAgICAgICAgICA8TG9jYXRpb24+DQogICAgICAgICAgICA8QWRkcmVzcz4xMC4xMDAxL2phbWFvdG8uMjAxNC4xPC9BZGRyZXNzPg0KICAgICAgICAgICAgPExvY2F0aW9uVHlwZT5FbGVjdHJvbmljQWRkcmVzcz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