—2—（液相色譜—二極管陣列法）1.2用于紫外光譜圖庫(kù)之外的具有紫外吸收的非法添加色譜條件與系統(tǒng)適用性試驗(yàn)用十八烷基硅烷鍵合硅膠為填充劑（ACQUITY液（99:1）為流動(dòng)相B，按下表進(jìn)行線性梯度洗脫；流速每分鐘0.45ml；二極管陣221122供試品溶液的配制中藥固體制劑取50mg，加測(cè)定法取供試品溶液2μl，注入液相色譜儀，同時(shí)記錄色譜圖與光譜圖。通過(guò)色譜條件與系統(tǒng)適用性試驗(yàn)用十八烷基硅烷鍵合硅膠為填充劑（AlltimaC18，胺0.5ml，用飽和氫氧化鈉溶液調(diào)pH值至7.0）:甲醇（70:30）為流動(dòng)相；流速每色譜條件與系統(tǒng)適用性試驗(yàn)用十八烷基硅烷鍵合硅膠為填充劑（AlltimaT3，色譜條件與系統(tǒng)適用性試驗(yàn)用十八烷基硅烷鍵合硅膠為填充劑（AtlantisC18，—4—表進(jìn)行線性梯度洗脫；流速每分鐘1.0ml；二極管陣列檢測(cè)器，采集波長(zhǎng)范圍為00堿堿堿銨索新素/素AU6.392峰6.392峰10.640.480.320.160.00200.00250.00300.00350.00400.00納米AU0.40205.40.350.300.250.200.150.100.05268.10.00200.0250.0300.0350.0400.0AU7.006峰10.60205.40.500.400.300.200.100.00200.0250.0300.0350.0400.0納米AU7.605峰1204.20.640.48芐星氯唑西林0.640.480.320.160.00200.00250.00300.00350.00400.00納米AU0.60.6204.21.945峰10.50.50.4229.00.30.2272.80.1272.80.0200.0250.0300.0350.0400.0納米酯酯酯芐AU4.408峰1203.0頭孢氨芐0.60頭孢氨芐0.45260.80.300.15342.70.00200.00250.00300.00350.00400.00納米頭孢洛寧頭孢洛寧—20—酸—21——22——23——24——25——26—200.0250.0300.0350.0喹酸7.194峰7.194峰10.2噁喹酸0.1219.5321.70.0200.0250.0300.0350.0400.0—27——28—啶—29—嗪唑啶嗪啉AU1.495峰1.495峰1205.40.500.400.300.200.100.00200.0250.0300.0350.0400.0200.0納米辛AU5.874峰15.874峰10.60269.3磺胺地索辛0.500.400.300.200.100.00200.0250.0300.0350.0400.0納米啶醇素AU4.742峰10.5207.70.4林可霉素0.30.20.10.0200.0250.0300.0350.0400.0納米素素AU15.524峰201.8358.115.524峰201.8358.10.440.330.220.11200.00250.00300.00350.00400.00納米242.0280.0347.6AU1264.4250.00300.001264.4250.00300.00350.00400.000.450.300.150.00200.00納米胍—40—唑AU0.600.45260.811.714峰0.600.45260.8204.20.300.150.00200.00250.00300.00350.00400.00納米唑納米—41—唑AU9.885峰19.885峰10.50恩康唑0.400.300.200.10271.70.00200.0250.0300.0350.0400.0納米酮—42—9.859峰9.859峰10.40.30.2294.30.10.0200.0250.0300.0350.0400.0唑0.87.16113.10.70.6氧阿苯達(dá)唑0.50.40.3293.10.20.10.0200.0250.0300.0350.0400.00.40.4212.59.144峰1247.90.30.2312.20.10.0200.0250.0300.0350.0400.0—43—唑245.4245.4294.1207.70.40.3非班太爾200.0250.0300.00.40.3非班太爾200.0250.0300.0350.0400.0213.6283.60.20.10.0啶0.4288.3365.6200.0250.0300.0350.0400.0236.17.169峰0.4288.3365.6200.0250.0300.0350.0400.0236.10.50.30.20.10.0AU12.002峰12.002峰1206.60.800.700.600.500.400.300.200.100.00253.8200.0250.0300.0350.0400.0200.0納米—44—12.8550.2204.2200.0250.0300.012.8550.2204.2200.0250.0300.0350.0400.0244.40.50.40.50.30.10.0—45—12.442峰1244.40.40.3乙酰氨基阿維菌素0.2205.40.10.0200.0250.0300.0350.0400.0205.4205.4244.4—46—AU10.656峰10.656峰10.600.450.300.15279.80.00200.00250.00300.00350.00400.00納米AU10.862峰1204.20.500.400.500.400.300.200.100.00264.5200.0250.0300.0350.0400.0200.0納米AU261.010.894峰10.50206.6AU261.00.400.400.300.200.100.00200.0250.0300.0350.0400.0200.0納米—47—脒AU200.00250.00300.00350.00200.00250.00300.00350.00400.00204.20.64287.0雙甲脒0.480.320.160.00納米磷294.3230.2294.3230.2AU200.00250.00300.00350.00400.00207.79.646峰1200.00250.00300.00350.00400.00207.70.480.360.240.120.00納米醚AU0.35205.4271.70.100.050.00200.0250.0300.0350.0400.00.35205.4271.70.100.050.00200.0250.0300.0350.0400.00.400.300.250.200.15納米—48—AU200.0250.0300.0350.0400.0200.0250.0300.0350.0400.0264.50.40甲氧普烯0.300.200.100.00納米0.4200.0250.0300.0350.00.4200.0250.0300.0350.0400.0212.50.50.30.20.10.0AU211.3211.3284.7369.2369.2唑—49—唑胍唑AU0.560.4211.0090.560.4211.009峰10.28240.70.280.140.00200.00250.00300.00350.00400.00納米AU205.4250.30.500.400.300.200.100.00200.0400.0250.0300.0350.0200.0400.0205.3244.3二硝托胺納米啉AU234.9203.00.50266.9鹽酸氨丙啉0.400.30234.9203.00.50266.9鹽酸氨丙啉0.400.300.200.100.00200.0250.0300.0350.0400.00.60納米AU0.500.40氫溴酸常山酮313.4200.0250.00.500.40氫溴酸常山酮313.4200.0250.0300.0350.0400.0243.20.300.200.100.00納米酸近芬芬芬鈉生AU0.608.308峰0.608.308峰10.45243.10.300.150.00200.00250.00300.00350.00400.00納米AU289.38.570峰289.38.570峰10.400.300.200.100.00200.00250.00300.00350.00400.00納米AU8.678峰1205.40.40243.2維他昔布0.300.200.100.00200.0250.0300.0350.0400.0納米芬AU0.200.20300.20.150.100.050.00200.0250.0300.0350.0400.09.275峰9.275峰10.400.350.300.25納米AU7.812峰1211.30.40格拉匹侖0.300.200.100.00200.0250.0300.0350.0400.0納米AU10.098峰10.098峰10.60285.8氟尼辛葡甲胺0.400.200.00200.00250.00300.00350.00400.00納米松鈉松松8.8678.867峰10.50.4氫化可的松0.30.20.10.0200.0250.0300.0350.0400.00.49.5590.49.559峰10.30.2204.20.20.10.0200.0250.0300.0350.0400.0399.0204.2399.0296.5AU8.130峰8.130峰10.800.70匹莫苯丹0.60266.90.50207.70.400.300.200.100.00200.0250.0300.0350.0400.0納米AU11.38911.389峰1237.30.400.300.200.100.00200.0250.0300.0350.0400.0納米胺鈉AU7.720峰1205.40.500.400.300.20