2025/07/23TypesandFundamentalsofELISATechniqueReporter:CONTENTSCatalogue01TypesofELISAMethod02BasicPrinciplesofELISA03ApplicationScenariosofDifferentELISAMethodsTypesofELISAMethod01DirectELISADirectELISADirectELISAemploysthecoatingofamicroplatewithanantigen,subsequenttowhichanenzyme-taggedantibody,capableofdirectlyconnectingwiththeantigen,isadded.TheprocessismorestraightforwardandinvolvesfewerstepsthanotherformsofELISA.IndirectELISAIndirectELISAMethodologyIndirectELISAdetectsantigensbyusinganenzyme-labeledsecondaryantibodythatbindstotheprimaryantibody,providingameasurablesignal.IndirectELISA'sKeyComponentsThemethodinvolvescoatingtheplatewiththeantigen,addingtheprimaryantibody,andthenasecondaryantibodyconjugatedwithanenzyme.IndirectELISATheIndirectELISAMethodologyTheprocessentailsfixingtheantigen,followedbyincubationwiththeprimaryantibody,thenthesecondaryantibody,andfinallyaddingasubstrateforcolormanifestation.ApplicationsofIndirectELISAIndirectELISAiswidelyusedinresearchfordetectingantibodiesinserum,measuringantigen-specificantibodies,andindiagnostictests.SandwichELISASandwichELISASandwichELISAemploysapairofantibodies,eachrecognizingauniqueepitopeonthetargetantigen.Oneantibodyisattachedtoasolidsurface,whiletheotheristaggedwithadetectablemarker,enablingthedetectionandmeasurementoftheantigen.CompetitiveELISACompetitiveELISAIncompetitiveELISA,there'sacompetitionoccurringbetweentheantigenwithinthesampleandanenzyme-taggedantigenintermsofbindingwithanantibody.Thehighertheamountofantigendetectedinthesample,thelesserthequantityofthelabeledantigenwillattachtotheantibody,whichconsequentlyleadstoareducedsignal.BasicPrinciplesofELISA02Antigen-AntibodySpecificBindingMasteringthefundamentalsofantigen-antibodyconnectionAntigensserveasstimulicapableofinitiatinganimmunereaction,whereasantibodiesareproteinssynthesisedbytheimmunesystemtocounteractantigens.ThedistinctivenessintheirinteractionformsthebasisofELISA.SpecificBindingofAntigenandAntibodyAntigen-antibodyinteractionisfundamentalinELISAprocedures.Enzyme-LinkedImmunosorbentAssay(ELISA)usesthisspecificbindingtodetectandquantifyantigensorantibodiesinasample,makingitcrucialtograsptheprinciplesofthisinteraction.Antigen-AbSpecificInteractionRoleofenzymesinsignalamplificationThereporterenzymelinkedtotheantibodyorantigenconvertsthebindingprocessintoadetectablesignal,enhancingitforanalysisandquantitationinanELISA.EnzymeMarkingandSubstrateInteractionCompetitiveELISAThecompetitiveELISAprocessentailsacompetitionforbindingbetweentheantigenpresentinthesampleandanenzyme-taggedantigen,alloccurringatafinitenumberofantibody-bindingsites.ELISA技術(shù)應(yīng)用的多樣場(chǎng)景03RapidScreeningELISAMethodDirectELISADirectELISAproceduresentailapplyingtheantigentothemicroplatesurface,thenintroducinganenzyme-linkedantibodywhichattachestotheantigendirectly.AntibodyDetectionviaIndirectEnzyme-LinkedImmunosorbentAssaySandwichELISASandwichELISAemploysadual-antibodysystemforantigenidentification,wheretheinitialantibodybindstotheantigenandthesubsequentantibodyconfirmsitspresence,ensuringbothhighspecificityandsensitivity.AntibodyDetectionviaIndirectEnzyme-LinkedImmunosorbentAssayDirectELISATheELISAtechnique,knownasdirectELISA,usesasingleantibodytodirectlyconnectwiththeantigen,followedbydetectionviaanenzyme-linkedantibody,renderingtheprocesssimple.AntibodyDetectionviaIndirectEnzyme-LinkedImmunosorbentAssayIndirectELISATheindirectELISAemploysaprimaryantibodyforantigenbindingandasecondaryantibodyfortheprimaryantibody'sdetection,providingversatilityandsignalamplification.AntibodyDetectionviaIndirectEnzyme-LinkedImmunosorbentAssayCompetitiveELISAELISA競(jìng)爭(zhēng)法通過(guò)檢測(cè)已知量標(biāo)記抗原與抗體結(jié)合的抑制能力來(lái)測(cè)定抗原含量，適用于小分子物質(zhì)。AntigenQuantificationviaSandwichELISATechniq