第1題TheHGP(HumanGenomeProject)wasfinishedin:A1990B2001C2008D2006第2題WhichofthefollowingtechniqueisdevelopedbyPaulBergwhoisnamedas“thefatherofmoderngeneticengineering”:ATechniqueofhybridization.BTechniqueofrecombinationDNA.CTechniqueofmonoclonalantibody.DPCR.

第3題WhichoneinthefollowingcanbeusedforDNAsequencing:AGersangmethod.BX-raydiffractionmethod.CSangerdideoxy-mediatedchainterminationmethod.Dsite-mutagenesis.第4題TwoimportantexperimentstoprovethatDNAisgeneticmaterialare:virulencetransformationexperimentofDiplococcuspneumoniaandinfectionexperimentofT2phagetoE.coli.Thekeypointofthesetwoexperimentsis:ADNAisolatedfrominfectedorganismscanbeusedaspathogenicagents.BItisDNAmutationthatcausethelossofvirulence.CForeignDNA(notprotein)absorbedbyorganismschangedgeneticpotency.DDNAcannotbetransferredbetweendifferentorganisms,soitmustbeaveryconservativemolecule.EDNAofeukaryotes,prokaryotesandviruscanbemixedandreplacedwitheachother.第5題In1953,WatsonandCrickfoundthat:ATwopolynucleotidechainsformdoublehelixstructureofDNAbyhydrogenbondlinkage.BDNAreplicationissemi-conservativeandusuallyformheteroduplexDNAconsistedofoneparentalchainandonedaughterchain.CThreecontinuousnucleicacidsconsistofageneticcode.DGeneticmaterialisusuallyDNAinsteadofRNA.第6題ThefindingofOkazakifragmentsprovesthatthereplicationofDNAis:Asemi-conservativereplication.Bcontinuousreplication.Cindirectreplication.Dsemi-continuousreplication.第7題Basedon“CentralDogma”,thedirectiontopassbiologicalinformationis:ADNA>Protein>RNABRNA>DNA>ProteinCDNA>RNA>ProteinDProtein>DNA>RNA第1題Thebasiccomponentofnucleicacidis:____AphosphateandriboseBnucleosideandnitrogenbasesCmononucleotideDnitrogenousbasesEdeoxyriboseandnitrogenbases第2題Toformanucleicacid,thelinkagebetweennucleotidesis:____AglycosidicbondBhydrogenbondC3’,5’-phosphodiesterDpeptidebondE2’,5’-phosphodiester第3題Thecontentofwhichchemicalelementisusuallyconstantinanucleicacid:____ACBOCNDHEP正確答案：E第4題Whichofthefollowingisnotincludedinanucleicacid:____ACBHCODSEN第5題WhichofthefollowingbasesisnotincludedeitherinDNAnorRNA:____AadenineBxanthineCguanineDthymineEuracil第6題Inthefreenucleotide,phosphategroupisusuallylocatedin:____AC1offive-carbonsugarBC2offive-carbonsugarCC3offive-carbonsugarDC4offive-carbonsugarEC5offive-carbonsugar正確答案：E第7題WhenDNAiscompletelyhydrolyzed,theproductsare:____AriboseandphosphateBdeoxyriboseandbasesCdeoxyriboseandphosphateDribose,phosphateandbasesEdeoxyribose,phosphateandbases正確答案：E第8題WhenDNAandRNAiscompletelyhydrolyzed,theproductsare:____AdifferentsugarandpartlydifferentbasesBdifferentsugarbutsamebasesCsamesugarandsamebasesDsamesugarandpartlydifferentbasesEdifferentsugarandcompletelydifferentbases第9題WhichofthefollowingisnotincludedinDNA:____AdAMPBdGMPCdCMPDdUMPEdTMP第10題Thelinkagebetweenfive-carbonsugar(shortedasR),bases(shortedasN)andphosphate(shortedasP)inanucleicacidis:____AN-R-PBN-P-RCP-N-RDR-N-PER-P-N第11題Basically,theprimarystructureofanucleicacidis:____AthesequenceofbasesinthepolynucleotidechainBthewayofbasespairinginthepolynucleotidechainCtheratioofbasesinthepolynucleotidechainDthewayofcoilingandfoldinginthepolynucleotidechainEthelinkagebetweengroupsinpolynucleotidechainC1-Lecture3-Homework第1題AboutthesecondarystructureofDNA,whichoneisfalse:____AThreehydrogenbondsareformedbetweenAandT,twohydrogenbondsareformedbetweenGandBNitrogenbasegroupsfacetheinsideofdoublehelixinDNA.CThereisbasestackingforcebetweenbasepairs.DTwostrandsareoppositelyoriented.ETherearemajorandminorgroovesinthesurfaceofdoublehelix.第2題WhichisthemainformofDNAnaturally:____AA-DNABB-DNACE-DNADZ-DNAENoneofabove.第3題ThesecondarystructureofDNAis:____Aα-helixBβ-sheetCβ-turnDdoublehelixErandomcoli第4題WhichofthefollowingisfalseaboutthecontentofbasesinDNA:____AA+T=C+GBA+G=C+TCG=CDA=TEA/T=G/C第5題WhichofthefollowingisnotincludedinDNA____AphosphodiesterBglycosidicbondChydrogenbondDdisulfidebondEVanderWaals'force第6題WhichofthefollowingisfalseaboutthedoublehelixstructureofDNA:____ADNAconsistoftwoanti-parallelstrands.BNitrogenbasesarestrictlypairedindoublehelix.CThesugarandphosphategroupsfaceoutwardofdoublehelix.DBaseplaneisperpendiculartothecentralaxis.EDNAisfoundintheright-handedconfigurationinallorganisms.正確答案：E第7題Basically,theprimarystructureofanucleicacidis:____AthesequenceofbasesinthepolynucleotidechainBthewayofbasespairinginthepolynucleotidechainCtheratioofbasesinthepolynucleotidechainDthewayofcoilingandfoldinginthepolynucleotidechainEthelinkagebetweengroupsinpolynucleotidechain第8題Whichofthefollowingisfalseaboutthestructureofnucleicacid:____ATherearemajorandminorgroovesinthesurfaceofdoublehelix.BBasestackingforceexistsbetweenneighboringbasepairsindoublehelixDNA.CDoublehelixstructureonlyexistinDNA.DDoublehelixstructurecanalsoexistinRNA.EComplementarybasepairingexistindoublehelix.第9題TheforcecontributingtoDNAstabilitymainlyinclude:____AphosphodiesterBhydrogenbondbetweenbasepairsCbasestackingforceDthesupportofphosphatebackboneEtherepulsiveforceofnegativelychargedphosphatebackbone正確答案：BC第10題WhichofthefollowingisrightaboutthedoublehelixstructureofDNA:____AA=TBNitrogenbasesfacetheinsideofdoublehelixinDNA.C10bpperturn,thepitchis3.4nmindoublehelix.DHorizontally,DNAstabilityismaintainedbyhydrogenbondbetweenbasepairs.EVertically,DNAstabilityismaintainedbybasestackingforce.正確答案：ABCDE第11題WhichofthefollowingiscorrectaboutthenitrogenbasesinDNA:____AA=G,C=TBThecomposingandratioofbasesinDNAisdifferentindifferentorganisms.CThecomposingandratioofbasesinDNAisdifferentindifferentorgansofoneorganism.DThecomposingofbasesinDNAisconstantwiththeageincrease.EUracilisincludedinDNA.正確答案：BDC1-Lecture4-Homework第1題WhichthefollowingisfalseaboutthetertiarystructureofDNA:____AKinkofthechainBSupercoilCLoopsformedwithinasinglestrandofDNADConcatenatedcircleincircularDNAEDoublehelixofDNA正確答案：E第2題WhichthefollowingisfalseaboutthesupercoilstructureofDNA:____AIt’sthecharacterofclosedcircularDNA(Onceasinglestrandordoublestrandsbreak,thesupercoilofDNAisdestroyed)BTopoisomerasesinthecellcanregulatethelevelofsupercoilofDNA.CTheformationofDNAsupercoilneedsDNAligase.DAlmostallDNAmoleculesinthecellisnegativelysupercoiled.EThelevelofsupercoilofDNAisregulatedbytopoisomerases.

第3題WhichthefollowingiscorrectaboutthetopoisomeraseII:____AItcanbreaktheglycosidicbondinDNAdoublestrands.BItdoesn’tconsumeATPwhenitworks.CItcanincreasethenumberofnegativelysupercoiledDNA.DItcanstabilizethetopologicalstructureofDNAmolecule.EItmakespositivesupercoiledDNA.第4題AcircularDNAwithBformreducesitscrossingnumberby1withthehelpoftopoisomeraseI,thenwhichthefollowingisfalse:____AThelinkingnumberofthisDNAisreducedby1.BNoATPisconsumedinthisprocess.CThisDNAcanmaintainitsrelaxedBform.DAnegativelysupercoiledDNAwouldbeformedifitmaintainsitsrelaxedBform.EThetwistingnumberofDNAwouldbekeepthesameifitmaintainsitsrelaxedBform.C1-Lecture5-Homework第1題ThehybridizationofnucleicacidcouldhappenbetweenDNAandRNA,DNAandDNA,then,whichofthefollowingRNAsequencecouldhybridizewithsinglestrandDNAwithsequence5’-CGGTA-3’:____A5’-UACCG-3’B5’-GCCAU-3’C5’-GCCUU-3’D5’-AUCCG-3’E5’-UAGGC-3’第2題WhichofthefollowingDNAmoleculehasarelativelyhigherTmvalue?____A5’-ATATCATATGATATGTA-3’B5’-CGGTACTCGTGCAGGT-3’第3題WhichisthemaximumUVabsorptionwavelengthofnucleicacids:____A280nmB260nmC240nmD220nmE490nm第4題WhichofthefollowingstatementiscorrectaboutthereasonthatnucleicacidhastheabilitytoabsorbUV:____ATheconjugateddoublebondbetweenpurineandpyrimidineBRiboseisconnectedtopurineandpyrimidineCTherearenitrogenatomsinsidepurineandpyrimidineDRiboseandphosphateisconnectedtopurineandpyrimidineEPhosphategroupisconnectedtopurineandpyrimidine第5題DNAdenaturationreferto:____ADepolymeriztionofpolynucleotidechainsBDNAmoleculeturnstodoublehelixfromsupercoilCThephosphodiesterbondintheDNAmoleculehasbeenbrokenDThehydrogenbondbetweenbaseshasbeenbrokenEThecompletehydrolysisofthenucleicacids第6題WhichofthefollowingstatementsarecorrectaboutTmvalueofDNA:____AItisdirectlyrelatedtothebasessequenceorderofDNABItisrelatedtothelengthofDNACItisdirectlyproportionaltothepercentageofG-CbasepairsDThehigherG+C/A+Tvalueis,thehigherTmvalueisETmvaluerepresentstheconcentrationofDNAafterdenaturation正確答案：BCD第7題Underthemeltingtemperature,whathappenedtotheDNAdoublehelix:____AThedoublehelixhasbeenuntiedcompletelyBThedoublehelixhasbeenuntiedabout50%CTheUVabsorptionat260nmincreasedDPartofhydrogenbondsbetweenbaseshavebeenbrokenEAlloftheG-Cbasepairsdisappeared正確答案：BCD第8題Whichofthefollowingreactionsareincludedinrenaturationprocess:____ATheformationofthehydrogenbondBTheformationofthephosphodiesterbondCTheformationofthephosphoesterbondDTheformationofthebasestackingforceETheformationofthecovalentbond正確答案：AD第9題WhichofthefollowingstatementaboutDNArenaturationiscorrect:____ATherenaturationtakesplaceamongtwocomplementarystrandsofthedenaturedDNAmoleculeBThebiggeroftheDNAmoleculeis,thelongeroftherenaturationtimewillbeCTheheat-denaturedDNAwouldrenatureonlyifitiscooleddownslowlyDThedenaturationcouldhappenamongDNAandRNAstrandsETheheat-denaturedDNAwouldquicklyrenatureunderthelowtemperature正確答案：ABC第10題Whichofthefollowingstatementaboutnucleicacidhybridizationiscorrect:____AItcouldhappenbetweentwoDNAstrandsfromdifferentsourcesBItcouldhappenbetweenDNAandRNAstrandsfromdifferentsourcesCItcouldhappenbetweenRNAstrandanditsencodedpeptideDHybridizationprocessisbasedontheDNAdenaturationandrenaturationEThehybridizationtechniquescouldbeusedtostudythestructureandfunctionofnucleicacids.正確答案：ABDEC1-Lecture6-Homework第1題Whatisthepurposeofusingliquidnitrogentofreezeandgrindthesampleduringgenomeextractionofplants:____AcompletegrindingofthesampleBpolysaccharideprecipitationCproteindenaturationDDNAprecipitation第2題WhatisthefunctionofCTABinthegenomeextractionofplants:____AcompletegrindingofthesampleBpolysaccharideprecipitationCproteindenaturationDDNAprecipitation第3題Whatisthepurposeofaddingorganicsolventmixtureofphenol/chloroform/isoamylalcoholduringgenomeextraction:____AcompletegrindingofthesampleBpolysaccharideprecipitationCproteindenaturationDDNAprecipitation第4題Whatisthepurposeofaddingisopropanol:____AcompletegrindingofthesampleBpolysaccharideprecipitationCproteindenaturationDDNAprecipitationandeliminationofsmallRNAmolecules.第5題WhichoffollowingisthebestchoiceforstorageofextractedDNAsample:____AultrapurewaterBTEbufferC75%ethanolDTBEbuffer第6題WhichofthefollowingstatementiscorrectaboutDNAagarosegelelectrophoresis:____AWhenmeltingthegel,ithastobeheatedthoroughly,withboilingforawhile,thenstopheatingtotransferthemeltedgelintothegeltank.BTheDNAbandscouldbeobserveddirectlyaftergelelectrophoresis,sincetheloadingbuffercontainsindicator.CLinearDNAmoleculeswithdifferentlengthholddifferentelectrophoreticmobility.DDNAmoleculeswithsamelengthholdthesameelectrophoreticmobility.Chapter1Test第1題Whatarethebasiccomponentsofanucleicacid:____AphosphateandriboseBnucleosideandnitrogenousbaseCmononucleotideDnitrogenousbaseEdeoxynucleosideandbase第2題Whatisthedirectionofgeneticinformationflowofreversetranscription:____ADNA→DNABDNA→RNACRNA→DNADDNA→proteinERNA→RNA第3題WhichofthefollowingstructurecausestheUVabsorptionofnucleicacids:____AThehydrogenbondbetweenpurineandpyrimidineBTheglycosidicbondbetweenbaseandpentoseCThephosphoesterbondbetweenpentoseandphosphateDTheconjugateddoublebondbetweenpurineandpyrimidineringEThephosphodiesterbondbetweennucleotides第4題Whatisthekeycomponentforstorageandtransmissionofgeneticinformationinnucleicacids:____AnucleosideBpentoseCphosphategroupDbasesequenceEpentose-phosphatebackbone第5題DNAmoleculedoesnotinclude:____AphosphodiesterbondBglycosidicbondChydrogenbondDdisulphidebondEVanderWaals'force第6題Belowarethetwomoleculesthathasbeenheat-denatured,whentemperatureofthesolutionwasdroppingslowly,whichonewouldbeeasiertorecover:____A5’-ATATCATATGATATGTA-3’B5’-CGGTACTCGTGCAGGT-3’第7題WhichofthefollowingstatementsaboutthestructureofnucleicacidisNOTtrue:____ATherearemajorandminorgroovesonthesurfaceofdoublehelixBThereisbasestackingforcebetweenupperandlowerlayersofbases

CThedoublehelixstructureonlyexitsinDNAmolecule

DThedoublehelixstructurealsoexitsinRNAmoleculeEThereiscomplementarybasepairingexistinginthedoublehelixstructure第8題DNAdenaturationmeans:____ADepolymeriztionofpolynucleotidechainBSupercoiledDNAmoleculeturntodoublehelixstructureCThephosphodiesterbondofthemoleculehasbeenbrokenDThehydrogenbondbetweenbaseshasbeenbrokenEThecompletehydrolysisofthenucleicacidmolecule第9題WhenrunningDNAagarosegelelectrophoresis,whichofthefollowingisNOTtrue:____AMeltedagaroseshouldbecooledforawhilebeforetransferredintogeltankBPutthesamplewellonthepositivesideoftheelectrophoresischamberandloadthesamplesCAfterthegelwasready,addelectrophoreticbufferandthenpulloutthecombDMixthesamplesolutionwithloadingbufferandthenloadtheminthesamplewellEUseethidiumbromide(EB)tostainthegelafterrunningandcheckthebandsunderUVlight第10題Underthemeltingtemperature,whathappenedtotheDNAdoublehelix:____AThedoublehelixhasbeenuntiedcompletelyBThedoublehelixhasbeenuntiedabout50%CTheUVabsorptionat260nmincreasedDPartofhydrogenbondsbetweenbaseshavebeenbrokenEAlloftheG-Cbasepairsdisappeared正確答案：BCD第11題Whichofthefollowingreactionsareincludedinrenaturationprocess:____ATheformationofthehydrogenbondBTheformationofthephosphodiesterbondCTheformationofthephosphoesterbondDTheformationofthebasestackingforceETheformationofthecovalentbond正確答案：AD第12題Whichofthefollowingstatementaboutnucleicacidhybridizationiscorrect:____AItcouldhappenbetweentwoDNAstrandsfromdifferentsourcesBItcouldhappenbetweenDNAandRNAstrandsfromdifferentsourcesCItcouldhappenbetweenRNAstrandsanditsencodedpeptidesDHybridizationprocessisbasedontheDNAdenaturationandrenaturationEThehybridizationtechniquescouldbeusedtostudythestructureandfunctionofnucleicacids正確答案：ABDE第13題WhichofthefollowingstatementisNOTtrueaboutthevirulencetransformationexperimentofDiplococcuspneumoniacarriedoutbyGriffithandAvery:____

AOnlyStypeofDiplococcuspneumoniahasthecapsulewhichissimilartocellwall.BInGriffith’sexperiment,ifStypestrainandheat-denaturedRtypestrainwasinjectedintomice,themicewouldnotdie.CThevirulencetransformationofbacterialstrainismainlyrelatedtothecapsuleoftheStypestrain.DThetransformationofaliveRtypestraintoStypestraincouldalsohappeninthetubebesideshappeninginvivointhemice.正確答案：BC第14題TheUVabsorptionvalueofnucleicacidisunrelatedtothepHvalueofsolution.()第15題ThecomponentofbasesinDNAfromdifferentbiologicaltissuesaredifferent.()第16題TheDNArenaturationnormallytakeplaceatatemperaturewhichisabout20degreelowerthanitsTmvalue.()第17題MostoftheDNAmoleculesarenegativelysupercoilednaturallyinorganisms.()第18題ForpurifiedDNAsample,thevalueofOD260/OD280lowerthan1.8indicatingthesamplecontainsRNA.()第19題TheTmvalueofDNAdecreasedwiththeincreasingof(A+T)/(G+C)value.C2-Lecture1-Homework第1題WhichofthefollowingstatementaboutageneisNOTtrue:____AThewholeDNAsequenceresponsibleforsynthesisofafunctionalproteinorRNAmolecule.BPolynucleotidesequenceofDNAmoleculethatcontainsspecificgeneticinformation.CTheminimalfunctionalunitofgeneticmaterial.DThegenesequenceconsistsofcontinuouslyarrangedtripletcodes.第2題Whichiscorrectaboutorganellegenome:____ASeveralcopiesofthegenecanbeincludedinoneorganelle.BConsistsofseveralchromosome.CContainsnumerousshortrepeatedDNAsequences.DDNAmoleculeinmitochondriaandchloroplastisusuallypackagedwithhistones,toformchromatinstructure.第3題WhichofthefollowingisNOTthecharacteristicofeukaryoticgenome:____AThegenomeofeukaryotesismuchbiggerthanthatofprokaryotes,withmultiplereplicationorigins,andthelengthofeachreplicaissmallerineukaryotes.BSomaticcellisusuallythediploidcellwithtwocopiesofhomologousgenome.CThenumberofcodingregionismorethannon-codingregion.DTheeukaryoticgenesareusuallydiscontinuoussplitgenesconsistingofexonsandintrons.第4題WhichofthefollowingisNOTthefocusofhumanstructuralgenomics:____AGeneticmapBTranscriptionmapCPhysicalmapDAnalysisofgenefunction第5題Whichofthefollowingstatementisthecharacteristicofprokaryoticgenome:____AContainsmultipleDNAreplicationorigins.BSinglechromosome,alwayscircular.CChromosomalDNAisalwaysfixedtobindingproteins.染色體DNA和蛋白質(zhì)固定地結(jié)合DConsistsofnumerousnon-codingsequences.第6題Thegenomeoftheviruscanbe:____AdsDNABssDNACdsRNADssRNA正確答案：ABCD第7題AgeneistheDNAsequenceencodingproteins().第8題ThechemicalbasisofmostgenesinnatureisDNA.()第9題Asplitgenereferstothattwogenesareseparatedbysomenon-codingDNAsequences.(

)第10題Plasmidcontainsoriginofreplicationandcanreplicatesindependentlyinthehostcell.()第11題ChromosomalDNAoftheeukaryotesiscircularincell.(

)C2-Lecture2-Homework第1題ThelengthofDNAfragmentineachnucleosomeisabout:____A100bpB200bpC300bpD400bp第2題Thehistonewithmostspeciesspecificityinthenucleosomeis:____AH1BH2ACH3DH4第3題Thebasicunitofeukaryoticchromosomeis:____A30nmfiberBNucleosomeCCoreparticleofnucleosomeDHistoneoctamer第4題WhichofthefollowingisNOTincludedinthecorehistoneoctamer:____AH1BH2ACH3DH4EH2B第5題WhichofthefollowingstatementaboutthenucleosomeisNOTture:____AAbout146bpofDNAsequenceisincludedineachnucleosome.BConsistsofchromatosomeandlinkerDNA.CCorehistonescontainH2A,H3,H4andH2BDH1islinkerhistone.第6題AfterDNAispackagedintonucloesometoformbeads-on-a-string,thecompressionratiois:____A1/6B1/7C1/10D1/40E1/8400C2-Lecture3-Homework第1題Themaincomponentofchromosomescaffoldis:____AhistonesBnon-histoneCDNADRNA第2題Theloopdomainsofchromosomeconsistof:____ADNAfiberB10nmfiberC30nmfiberDChromatidfiberENoneoftheabove第3題Insolenoidmodelofchromosome,every()nucleosomesperturnareincludedinonesolenoid:____A2B4C6D8E10第4題Whichofthefollowingiscorrectabouttheassemblyofchromosome:____Anucleosome→corehistoneoctamer→solenoid→microstrip→loopdomain→chromatidBcorehistoneoctamer→nucleosome→solenoid→loopdomain→microstrip→chromatidCcorehistoneoctamer→nucleosome→microstrip→solenoid→loopdomain→chromatidDcorehistoneoctamer→nucleosome→solenoid→microstrip→loopdomain→chromatidEcorehistoneoctamer→nucleosome→loopdomain→solenoid→microstrip→chromatid第5題Therelationshipofchromosomeandchromatinis:____ATheyaresamesubstanceinthesameperiodofcellcycle.BTheyaredifferentsubstanceswithdifferentmorphologies.CTheyaresamesubstancewithsamemorphology.DTheyaresamesubstanceinthedifferentperiodofcellcycle.ENoneoftheabove第6題DNAiscompressedby()foldsfromdoublestrandmoleculeto30nmfiber:____A6B10C40D240E1000第7題WhichofthefollowingstatementisNOTtrueabouttheNterminalofhistoneanditsmodificationsaswellasitsfunctionsinnucleosomeassembly:____ATheNterminalofcorehistonecontainsseveralaminoacidresidueswithpositivecharge,whichcaninteractwithDNAmoleculeandstabilizethenucleosomes.BTheSerandThrresiduesintheNterminalofcorehistonecanbephosphorylated,toreducetheinteractionofhistoneandDNAmolecule.CTheacetylationintheNterminalofcorehistonecanstabilizetheinteractionofhistoneandDNA.DTheNterminalofcorehistonecaninteractwithhistoneoctameroftheneighboringnucleosome,tostabilize30nmfiberofchromosome.

第8題Whichofthefollowingstructuresorcomponentscontributetotheprocessthatnucleosomeformstable30nmfiber:____AFour-helixbundleformedbycorehistone.BNterminalofcorehistone.CHistoneH1DCoreparticleELinkerDNA正確答案：BCC2-Lecture4-Homework第1題WhichofthefollowingchromatinremodelingcomplexcanmakeDNAmoleculeslideoncorehistoneandshortenthedistanceofneighboringDNA:___ANuRDfamilyBISWIfamilyCSWR1DSWI/SNFfamily第2題WhichofthefollowingstatementaboutthelocationofnucleosomeisNOTtrue:____ATheinteractionsbetweennucleosomeandDNAaredynamic.BTheinteractionsofnucleosomeandDNAarenotsequencespecific.CAnucleosomeconsistsofDNAmoleculewithlengthofatleast150bp.DTheDNAsequencesdon’taffectthelocationofnucleosomeatall.第3題Whichofthestatementaboutthemodificationofhistoneiscorrect:____ATheacetylationofLycresidueintheNterminalofhistonecanstrengthentheinteractionbetweendifferenthistones.BChromodomaincanrecognizetheacetylationsiteofhistone.CTheacetylationandphosphorylationofhistonereducetheaffinitybetweenhistoneandDNA.DBromodomaincanrecognizethemethylationsiteofhistone.第4題WhichofthefollowingstatementaboutthechromatinremodelingisNOTtrue:____AItisaboutthechangeofthestructureandpositionofchromatin.BThelocationofnucleosomeinDNAstrandskeepsunchanged.CItiscloselyrelatedtothemodificationsintheNterminalofhistone.DThecomplexforchromatinremodelingpossessATPaseactivityandcanbreaktheinteractionofDNAandhistone.第5題Whichofthefollowingstatementsaboutthechromatinremodelingcomplexarecorrect:____AThechromatinremodelingcomplexconsistsofSWI/SNF,ISWIandSWR1family.BThechromatinremodelingcomplexcontainsdomainsforrecognitionofmodificationsiteofhistone.CSWR1familycanreplacetheH2A-H2BdimerofthenucleosometoH2A.Z-H2Bdimer.DISWIfamilycanmakeDNAmoleculeslideonthecorehistoneoctamer,changingthearrangementofnucleosomeandshorteningthedistanceofneighboringDNA.正確答案：ACBChapter2Tests第1題WhichofthefollowingstatementaboutgenomeisNOTcorrect:____AThewholenucleicacidinformationthatresponsibleforthesurvival,proliferation,developmentandactivitiesofanorganism.BBothchromosomalgenomeandorganellegenomecanbeincludedinaeukaryoticcell.CSomeregulationsequencesforgeneexpressionareincludedingenome.DThemostsequencesofeukaryoticgenomeencodeproteinproduct.第2題HIVthatcausesAIDSis:____ADouble–strandDNAvirusBSingle–strandDNAvirusCRNAvirusDPhage第3題WhichofthefollowingisNOTincludedinthehistonecoreoctamerofnucleosome:____AH1BH2ACH3DH4EH2B第4題DNAfragmentincludedinanucleosomecoreparticleis:____A55bpB146bpC166bpD200bp第5題What’sthefollowingstatementabouttheeffectofmodificationinhistoneterminalonnucleosomestabilityisNOTcorrect:____AThepositivechargedaminoacidresiduesinhistoneterminalbenefitforthestableconnectionofhistoneandDNA.BTheacetylationofLysresidueinhistoneterminalmakethepackagingof10nmfibermorecloser.CThephosphorylationofhistoneterminalreducesitspositivechargesandweakenstheinteractionofDNAandhistone.DTheacetylationandmethylationsiteofhistonecanbeusedtorecruitthechromatinremodelingcomplex,whichwouldaffecttheinteractionofDNAandhistone.第6題What’sthefollowingiscorrectabouttheloopmodel:____AItisformedbythefurtherfoldingof10nmsolenoidalfiber.BThecentralaxisofloopmodelconsistsofnon-histones.C16loopsareconnectedwithnon-histoneandradiallyarrangedinaplane,whichformsamicro-strip.DAbout108micro-stripsareincludedinachromatid.第7題Inthemodificationofhistone,Bromodomaincanrecognize:____ATheacetylationsiteofhistone.BThemethylationsiteofhistone.CThehistoneterminalthatarenotmodified.DNon-histone.第8題Thedifferencesofprokaryoticgenomecomparedtoeukaryoticgenomeare:____AItcontainsseveralDNAreplicationorigins.BSinglechromosomeandalwayscircular.CChromosomalDNAisnotconnectedwithhistonetoformnucleosome.DItcontainsnumerousnon-codingsequences.正確答案：BC第9題Whichofthefollowinghistonesarehighlyconserved:____AH1BH2ACH3DH4正確答案：BCD第10題Whichisincludedinachromatosome:____AHistonecoreoctamerBH1C55bplinkerDNAD146bpDNAE166bpDNA正確答案：ABD第11題Whichofthefollowingstructuresareincludedinthechromatin:____ANucleosomebeadsBSolenoidCLoopsDChromatid正確答案：ABCD第12題AgeneistheDNAsequencesencodingaprotein():()第13題ThegenomeofDNAvirusisdouble-strandDNA.()

第14題Thechromosomeofbacteriaonlyconsistsofasinglecirculardouble-strandDNA.()第15題MostofDNAsequencesinhigheukaryotesdon’tencodeproteins.()第16題DNAisgeneticmaterialbutRNAisnot.()第17題FourkindsofhistonesinnucleosomecoreparticleaswellasH1ishighlyconserved,withoutspeciesandtissuespecificity.()第18題Themaindifferenceofchromosomeandchromatinisthecompactness,whiletheirchemicalcompositionisexactlythesame.()第19題Thediameterofbeads-on-a-stringstructureofnucleosomeis30nm,towhichlinkerDNAisconnectedtoform30nmfiber.()第20題TheNterminalofcorehistoneisnecessarytoform30nmfiberofchromotin.()C3-Lecture1Homework第1題ThetechniquethatconfirmssemiconservativereplicationofDNAis:()ASangermethodBdensitygradientcentrifugationCα-complementationDDotblotEWesternblot第2題Adouble-strandedDNAfragmentthatisisotopicallylabeledwith15N,wasculturedforngenerationsinthemediumcontaining14N.Sothenumberof