第1題ThegeneticmaterialofalllivingthingsisDNA.第2題Allgenesencodeproteinorpolypeptidechains第3題SomestructuralgenesonlyencodeRNA第4題Thepromoterisdirectional.第5題TheretroviralgenomeisRNA.第6題Structuralcharacteristicsofthehumangenome

ALargenumberofrepetitivesequencesBGeneoverlapCOperonstructureD

PolycistronicmRNA第7題Genomeis

AThesumofthegeneticmaterialofacompletesetofhaploidsinacellBAllDNAinthecellCAllRNAinthecellDAllchromosomesinthecell第8題Whichofthefollowingisacis-actingelement

ApromoterB

intronC

exonD

operon第9題

ViralgenomestructurecharacteristicsAgeneoverlapBSomeviralgenomesareRNAC

SomearesegmentgenomeD

Largenumberofrepetitivesequences正確答案：ABC第10題Bacteriagenomestructurecharacteristics

AOperonstructureB

IsogeneCAnoriginofreplicationDgeneoverlap正確答案：ABC第1題MismatchrepaironlyrepairsthemismatchedbaseincorporatedduringDNAreplicationineukaryoticcells.第2題SpontaneoushydrolysisofbasescancausepurinesorpyrimidinestoshedfromtheDNAchainribosephosphatebackbone.第3題Inadditiontoeasilycausingtheformationofpyrimidinedimers,ultravioletradiationcanalsocausedamagesuchasDNAstrandbreaks.第4題Cross-linkinginDNAdamageonlyreferstocross-linkingbetweenDNAmolecules.第5題DNAdamagecausedbyexogenouschemicalsismainlyrepairedbymismatchrepair.第6題UltravioletradiationcausesdimersbetweenthebasesofDNAmolecules,themostcommonformisAT-TBC-CCC-T

DT-U第7題IntheprocessofE.colimismatchrepair,themismatchednucleotidesareidentifiedbyAmutSBRecJCmutLD

mutH

第8題Whenadenineexistsintheformofiminotautomer,itcanbepairedwith

ACytosineBThymineCGuanineDHypoxanthine第9題IntheprocessofE.colinucleotideexcisionrepair,theroleofUvrBis

AIdentifythedamagesiteB

Unwindthedoublestrand

CCutson3ˊsideDCutson5ˊside正確答案：BC第10題DNAdamagecausedbyalkylatingagent

ABasealkylation

BBasesheddingC

DNAstrandbreakD

Pyrimidinedimer正確答案：ABCUnitassignmentforweek4第1題TheyieldofPCRproductsisonlyrelatedtothenumberofinitialtemplates.第2題Inthereal-timequantitativePCRreaction,theCtvalueofeachtemplatehasalinearrelationshipwiththelogarithmoftheinitialcopynumberofthetemplate.Thehighertheinitialcopynumber,thesmallertheCtvalue.第3題ThesecondaryantibodyusedinWesternblottingistoprotecttheprimaryantibody,therebyimprovingthesensitivityoftheprimaryantibody.第4題cDNAlibraryreferstoallcDNAclonesinaspecificcellandinaspecificstate.

第5題VectorisaDNAmoleculethatcancarrytheforeignDNAsegmentsandmakeitreplicateorexpressinthehostcell.

第6題

PlasmidsusuallyA

existwidelyinavarietyofmicroorganisms,includingbacteriaandyeastBcan'tbeinheritedinastablewayoutsidethechromosomesofhostcells.C

areextrachromosomalsingle-strandedcircularDNAmoleculesofthebacterialcell.Darevectorsforgenecloning,andtheymayholdforeignDNAfragmentsmorethan10kb.第7題NorthernblothybridizationisthatAItcanidentifythecontentandsizeofspecificmRNA.B

Itcandigestthenucleicacidtobetestedwithrestrictionendonucleases.CItcantransfertheprobetoasolidsupport.DItisaDNAanalysistechnology.第8題WhichisthecorrectdescriptionofSangersequencing?

A

ddNTPsubstrateterminatetheextension.BUsinghightemperaturetoterminatetheextensionreaction.C

ItisbasedonRNAstrandsynthesisreaction.DThereactionsubstrateisdNTPorNTP.第9題Reversetranscriptase

Ahas5ˊ→3ˊpolymeraseactivityBhas3ˊ→5ˊexonucleaseactivityCisanRNA-dependentDNApolymeraseDisaDNA-dependentDNApolymerase正確答案：AC第10題Processofgenecloningincludes

A

preparationoftargetgeneandappropriatevectorBinsertingtargetgeneintovector

C

introductionofrecombinantDNAintohostcellsDscreeningforandidentifyingrecombinantDNA正確答案：ABCD第11題

WhichofthefollowingiscorrectaboutPCR?

A

Theamplifiedfragmentisbeyondapairofprimers.BItisakindofinvitroamplificationtechnologyofDNAfragments.CTheproducthasbeenaccumulatingexponentially.DItincludesthreebasicprocesses,namelydenaturation,annealingandextension.正確答案：BCD第12題

Whichofthefollowingiscorrectaboutthegenechip?AItcansimultaneouslyanalyzethevariationofmultiplegenes.BMicroarraysampledensityisverysmall.C

Itcanbeusedtostudytheexpressionofmultiplegenes.DItisaregularlyarrangedcDNAoroligonucleotidearray.正確答案：ACDUnitassignmentforweek5第1題SDSisanelectrophoretictechniquetoseparateproteinsaccordingtotheirpolarity.第2題Proteinchipcanbeusedforantigen-antibodyscreening.第3題TheresultsofproteinseparationinSDSandnon-denaturingelectrophoresisarethesame.第4題Isoelectricfocusingisatechniquetoseparateproteinsaccordingtotheirmass-to-chargeratio.第5題Theyeasttwo-hybridsystemtostudyproteininteractionsrequirestheseparationoftargetproteins.第6題TheincorrectdescriptionabouttheprincipleofSDSis

A

SDScanseparateproteinswithdifferentisoelectricpoints.BSDSisananionicdetergent.CSDScanbecombinedwiththehydrophobicpartofprotein,sothattheproteincanobtainnegativecharge.DThemobilityofproteinsinSDSarerelatedtotheirmolecularsizes.第7題Theexperimentaltechniqueusedtostudyproteininteractionsis

Ayeasttwo-hybridizationB

InsituhybridizationCSAGE

Ddotblothybridization第8題Asameansofhigh-throughputproteinanalysis,themainadvantagesof2-DEliesin

ATheresultsof2-DEcontainingthousandsofproteinspotsharbouringdifferentisoelectricpointand/ormolecularsize.Bcontinuousandautomatedsamplepreparationandtwo-dimensionalgelelectrophoresis.Citscapabilityindetectingparticularlylowprotein.Ditscapabilityindetectinghydrophobicproteins.第9題Post-translationalmodificationsofproteinsincludeAglycosylationBmethylationCphosphorylationDacetylation正確答案：ABCD第10題Themainresearchmethodsofproteininteractionbasedonmassspectrometryinclude

AProteinchipBImmunocoprecipitationcoupledmassspectrometryCAffinitychromatographycoupledmassspectrometryDBiosensorcoupledmassspectrometry正確答案：ABCDUnitassignmentforweek6第1題Nonsensemutationistheproductionofmeaninglessproteinaftermutation.

第2題Missensemutationscansignificantlyaffectproteinfunctionandphenotype,leadingtodisease.第3題Restrictionendonucleaseisabase-specificendonuclease.

第4題Whichofthefollowingmethodsisnotincludedingenediagnosis?AGenetargeting

BPCR

CNucleicacidhybridizationDGenechip

第5題Whichiscorrectaboutindirectgenediagnosis

ADetectionoflinkagegeneticmarkersBDetectionofgeneexpressionproductsCBetterthandirectdiagnosisDThepathogenicgenemustbeknown第6題ThefollowingstatementaboutgenerecombinationisincorrectAGenerecombinationoccursonlyduringsexualreproductionBGenerecombinationcanproducemultiplegenotypesCNonallelesonnonhomologouschromosomescanundergorecombinationDGenerecombinationisthefundamentalsourceofbiologicalvariation第7題Thenecessarycasefordirectgenediagnosisis

AThenormalmolecularstructureofthetestedgenehasbeenidentifiedBThereisadirectcausalrelationshipbetweengenemutationsandthediseaseCThemutationsiteofthetestedgeneisknownDThereisnoothergenemutationsinthetestedsample正確答案：ABC第8題Genemutationismainlyachangeofdnaprimarystructure,whichcanbeachangeofsinglenucleotide,orachangeofmultiplenucleotidesorevenasequenceofnucleotides;itsmolecularmechanismcanbereplacement,insertionordeletion,etc.,thusproducingdifferentmutationtypessuchas

APointmutationBDeletionmutationCInsertionmutationDReverseormigrateinversion正確答案：ABDC第9題ThetargetmoleculeofgendiagnosisisjustDNA.

第10題Southernblottingcanbeusedforthediagnosisofgenedeletionmutations.Unitassignmentforweek7第1題RibozymeisaproteinmoleculethatcanshearsmallmoleculeRNAs.第2題Adenovirusvectorsarecapableofsiteintegration.

第3題Geneinterventioncanincreasetheexpressionofagenetotreatdisease.第4題Distanttumorcellscannotbeaffectedbysuicidegenebystandereffects.第5題Thereisnopromotersequencesinretroviralpre-virusLTR.第6題Whatisthecorrectdescriptionaboutthesuicidegenetherapystrategy？AThesuicidegeneshouldhavepost-introductionexpressionintherecipientcells.BDirectlyinh