1、均勻設計法研究八角油-環(huán)糊精包合物的制備工藝<    【關鍵詞】   八角油； -環(huán)糊精； 包合物制備； 均勻設計Abstract:ObjectiveTo study the inclusion complex of Star anise oil with cyclodextrin. Methods3 factors and 6 levels of conditions for the preparation of the inclusion complex were selected by the uniform design. The

2、 ratio of Star anise oil in the inclusion complex was used as criterion in these experiments. The inclusion complex was made by pestling method. ResultsThe best conditions of the inclusion complex were that: the ratio of Star anise oil and -Cyclodextrin was 1:13.0(mL/g), and grinding for 4.0 hours a

3、t 35.0. The ratio of volatile oil in the inclusion complex was about 81.4%, and the inclusion complex was identified by IR. ConclusionThe optimized process is stable and highly efficient.   Key wordsStar anise oil；-Cyclodextrin；  Inclusion process；Uniform design    Guan

4、gxi is a Chinese autonomous province, located in a subtropical zone, south west of China. In particular, it has extensive resources of different plants. Among them, there is a special and commercial product, essential oil, which is widely recognized. Essential oils are the volatile, fragrant substan

5、ces residing in the flowers, fruits, leaves, and roots of many plants1.Most of them are a complex mixture of chemical constituents，including nitrogen and sulfur-containing molecules, aromatic chemicals, and terpenes    The following oils are the most famous and are found in large quan

6、tity in Guangxi. Eucalyptus oi1 has been employed in ointments as a topical counter-irritant and together with menthol as an inhalation1.Star anise oil is the main product in the fruits of Illicium verum. The plant contains remarkable essential oil, which is used as flavoring and medicine to control

7、 caught2.The related content of trans-Cinnamaldehyde in Guangxi cinnamon oil was 11.58%, higher than that in Yunnan cinnamon oil. These two provinces are the main producing areas of cinnamon oil in China 3.    Star anise oil is separated from the fruits of Illicium verum Hook.f. This

8、plant is used as flavorings as well as Chinese herbal medicine. In Star anise oil, anethole(structure below) is the major component which has structures as the Cis & trans isomers occurring in nature. Remarkably, the Trans isomer is always b Ei ng more abundant. Star anise oil has a distinct eff

9、ect on raising leucocytes (the white blood-cells), so in American patent, it has been applied in the remedy for anti-leukemia. And it also has been used as poultry feed additive composition4. However, the effectiveness decreases with long time storage, and both physical and chemical characteristics

10、change relatively.-CyclodextrinX(structure above) has been of interest in numerous industries including the pharmaceutica1 field. This is due to th EI r particular structures which impart interesting physiochemical properties. Today -CD is known for their ability to molecularly encapsulate a wide va

11、riety of the drugs into their hydrophobic cavity, resulting in the enhancement of water solubility and drug dissolution's rate1. The results of physi cochemical tests showed that after inclusion the solubility and stability of paprika pigment, the binding ability of -cyclodextrin for inclusion c

12、omplex with guest curcumin, was improved obviously57.So it is considered as a good supplementary material for injection8.    Up to date, although the uniform design has been widely used in many synthesis and separation fields9,no reports related with the inclusion complex of Star anis

13、e oil and-cyclodextrin, by three factors and six levels uniform design, has been available. However, by using this practical design, the value of Star anise oil would definitely enhance and would benefit many pharmaceutical and commercial industries. 1 Reagents     -Cyclodextrin: was

14、obtained from Pharmaceutical Manufacturing Factory.Shanghai;Star anise oil: was abstracted from the fruits of Illicium verum Hook.f. All the other chemicals and reagents used were of the reagent grade obtained commercially.2  Methods 2.1  Use Uniform Design for the experimentsAmong many fa

15、ctors that effect the preparation of the complex, the temperature, the ratio of oil and CD, and the time to mix two compounds, are most important10.Hence, U*（63）three factors and six levels of the uniform design was considered. To see details, in table 1.2.2  Researches in the inclusion complex

16、2.2.1  The complex formation: Certain amount of -CD was dissolved in 25 times of water, l ml of oil was deluded by 1ml of alcohol, and the physical mixture of the oil and -CD was also prepared in the different ratio by gradually mixing both, ground for 1.54.0 hours at the same temperatureThe co

17、mplex was filtered and the residue (complex) was washed with 30 ml of absolute petroleum ether, 3 timesFinally kept in the desiccators until dried and the weight then recorded.2.2.2  The instrumental error: 1 ml of pure oil was added in 500mL bottle. And followed the steps described by Chinese

18、Pharmacopoeias (2000), the first method,calculated the error with following form and the result was 70.0%.    The instrumental error= (the volume of the pure oil after boiling/the volume of the pure oil before boiling)×100%2.2.3  Evaluation of the complex: The -CD and Star anise oil mixture was evaluated by the ratio of Star anise oil. And the