1、人肌紅蛋白基因外顯子一的生物信息學(xué)分析一IntroductionIntroduction ：Myoglobin, the article is composedof a peptide chains and a binding protein of hemeprostheticgroup,iswithinthemuscleoxygenstorageprotein,itsoxygensaturationoftheellipticcurve.Myoglobinisthemainproteinofskeletalmuscleandcardiacmuscle,whenmuscledamage, lea

2、kage to the circulating in the blood inmuscle tissue, increase the serum concentration ofmyoglobin, the index is used to determine whethermuscle damage.二PurposePurpose：對(duì)人肌紅蛋白基因外顯子一基因序列使用 “Bioedit和 DNAMAN、anthe5.0”及相關(guān)網(wǎng)站進(jìn)行 GC 含量統(tǒng)計(jì)、電子酶切、引物設(shè)計(jì)、基因在人類基因組中的定位、電子克隆及蛋白在細(xì)胞內(nèi)的定位、氨基酸組成統(tǒng)計(jì)、分子量、等電點(diǎn)、親疏水性、信號(hào)肽分析（跨膜區(qū)域）

3、、二級(jí)結(jié)構(gòu)（含保守結(jié)構(gòu)域）及三維結(jié)構(gòu)分析，miR 分析，啟動(dòng)子分析等。其中抗原表位分析、多序列比對(duì)和進(jìn)化分析等。三The nature and function of myoglobin：Nature: myoglobin = a polypeptide chain +a prostheticgrouppolypeptidechain,composedof153aminoacidresiduesProsthetic group: heme prosthetic groupMolecular weight: 16, 700Function:transportofoxygenandoxygenst

4、oragefunction in muscle tertiary structure of myoglobin四Key word:肌紅蛋白（myoglobin）五五 ImplementsImplements andand methodsmethods：采用 Bioedit 和 DNAMAN 分析軟件工具和在線有關(guān)蛋白質(zhì)和核酸方面的分析軟件，分別進(jìn)行核酸和蛋白質(zhì)兩個(gè)方面的分析。核酸分析首先從 pubmed 核酸數(shù)據(jù)庫中搜索到人的 Myoglobin 核酸序列，分別進(jìn)行序列的基本性質(zhì)分析，限制性酶譜分析及其基因在染色體上的定位分析。具體操作方法將根據(jù)上述各個(gè)軟件上“Help”文件進(jìn)行，基因在染色體

5、上的定位分析根據(jù)網(wǎng)頁上的操作說明進(jìn)行。從 pubmed 蛋白質(zhì)數(shù)據(jù)庫中搜索到人的 Myoglobin 和整個(gè)前體 Myoglobin蛋白質(zhì)序列，分別進(jìn)行蛋白序列的基本性質(zhì)分析（氨基酸組成、分子量、等電點(diǎn)） ，親疏水性分析，蛋白酶切位點(diǎn)分析，信號(hào)肽序列分析，二級(jí)結(jié)構(gòu)和三維結(jié)構(gòu)分析，同源序列和進(jìn)化分析。二級(jí)結(jié)構(gòu)分析包括結(jié)構(gòu)功能域、Motif、保守位點(diǎn)等，具體操作方法將根據(jù)上述各個(gè)軟件上“Help”文件和網(wǎng)頁上的操作說明進(jìn)行。Resluts:1.從 NCBI 中可以得到一下有關(guān) Myoglobi 蛋白分子的信息：Human myoglobin gene, exon 1GenBank: M10090.

6、1LOCUSHUMMGI12552 bpDNAlinearPRI 07-JAN-1995DEFINITIONHuman myoglobin gene, exon 1.ACCESSIONM10090VERSIONM10090.1GI:187580KEYWORDSFok family repetitive sequence; direct repeat; myoglobin;repeatregion; tandem repeat.SEGMENT1 of 3SOURCEHomo sapiens (human)ORGANISMHomo sapiensEukaryota; Metazoa; Chorda

7、ta; Craniata; Vertebrata;Euteleostomi;Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;Catarrhini; Hominidae; Homo.REFERENCE1(bases 1 to 2552)AUTHORSAkaboshi,E.TITLECloning of the human myoglobin geneJOURNALGene 33 (3), 241-249 (1985)PUBMED2989088COMMENTOriginal source text: Human: fetal

8、liver DNA (library ofLawn etal.), clone lambda-HMB151; delta-thalassemia leukocyte DNA(libraryof Kimura et al.), clone lambda-HMB159.Draft entry and computer-readable copy of sequence 1kindlyprovided by E.Akaboshi, 22-OCT-1985.A tandem repeat sequence is found at position 142-794.Position ofthe mRNA

9、 cap site was deduced from that of the sealmyoglobin gene(Blanchetot et al. 1983).FEATURESLocation/Qualifierssource1.2552/organism=Homo sapiens/mol_type=genomic DNA/db_xref=taxon:9606/map=22q11.2-qterrepeat_region1100.1750/note=Fok family repetitive elementprim_transcript 1895.2552/note=myoglobin mR

10、NA and introngenejoin(1965.2552,M14602.1:1.2671,M14603.1:1.501)/gene=MBCDSjoin(1965.2059,M14602.1:1415.1637,M14603.1:355.501)/gene=MB/note=myoglobin/codon_start=1/protein_id=AAA59595.1/db_xref=GI:386872/translation=MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEA

11、EIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAEGAMNKALELFRKDMASNYKELGFQGexon2552/gene=MB/note=myoglobin intron AORIGIN2 bp upstream of RsaI site.1 gtactgtatt ttcattcctc ttagttatct ccctaaaaag actctgagttccttgaacac61 aggaaggtgt tttatttgat tttgttatcc tcagcatgta gcagtgtctgacacacagta121 ggtgctctat cactgtgaga

12、 gggatggatg gatgggtgga gttacagatggatagaagga181 tagatggagg gatgggtgga tgatggatgg atagatggat ggaggggggatgatgaatgg241 agggataatg agtggatgaa tgagggaatg ggtggatgga tggatggagggatggaggaa301 cagatagata gatggaggga tgggtgggtg atggatggat agatggatggagggagggat361 gatgaatgga gggataatga atggatgaat gaggggatgg gtgga

13、tggatgaatggaggg421 atgatgggtg gatgaatgaa ttgagggatg gatggatgaa cacatggatggatggataga481 tggatagatg gaggaactgg tggattttgg atggatgggt ggatggatagatgaatgaat541 gcctggatag acaaagagat gatggataga tgaatagatg aattaagggatgtcggatag601 atggagggat tgatagatgt tggatggatg ggtggtggat ggatagatgagtgaatgcat661 ggatagaca

14、a agagatgatg gatggatgaa ttaagggatg acagatggatggatggatga721 gtaactggat ggacaagtgg ataaatggat agatggttga atacctgaatggattgaagg781 aggatgcatg gatgtaagat aaggctaatc atcctccact ctctttctttgcaaaaccat841 ccacccattt actcaataaa catttattca gttcaaactt ggcacaaagcaccatgtgag901 gcccaagaga tacgtgggtt aataaaacag agct

15、cctgcc ctcctgaaaactgcaaagaa961 aggggcgtgg cttcctgagt tcaaatccca actctgccag cgactagctgtacatcagtg1021 atgtttccct actttctctc aattaaatag ggataatgtc agtacctatcacattgggag1081 gtcttgcggg gattaaatga gttaccaaat gccaagtgtt tgggacagggcctggcaccc1141 agcaaagtct cttgtgagtg ctggctgcta ttatcctaat ggagaagatggcatgaaa

16、ac1201 caggaaatag gatgcccttt gggaagcaat gcaacaggaa cttacacaaagaaaggaaag1261 gaggaagcaa ttagtggtgt ctcaaaggag tatgtcaaga aaaacttttcagagggaaac1321 ctttgagcag ggtcatgaaa acaggagttc tctaagagat tgtggacttgcctgggacca1381 cctggctata agcacaaaac catccggttc ctttctgtca cttctggcgggtgaggggtc1441 tctggcaaag gggcag

17、aagg tgcgtgagag gttgcgaatg gccaggactgtcctggggcc1501 agccggggca cctggtggcc aagcttagaa acatgacagg tcctcttgggagggctgacc1561 gcagggagcg ttgggtttca ggctgctggc gtcggcttct gtggtgccctttctgtcggc1621 tatgagagtc cagacagtgc ccaacctcct ccccttcttt ccacacgcacaaccacccca1681 ccccctgtgg cctgagctgt cctgcctcgc cacaatgg

18、ca cctgccctaaaatagcttcc1741 catgtgaggg ctagagaaag gaaaagatta gaccctccct ggatgagagagagaaagtga1801 aggagggcag gggaggggga cagcgagcca ttgagcgatc tttgtcaagcatcccagaag1861 gtataaaaac gcccttggga ccaggcagcc tcaaacccca gctgttggggccaggacacc1921 cagtgagccc atacttgctc tttttgtctt cttcagactg cgccatggggctcagcgacg1

19、981 gggaatggca gttggtgctg aacgtctggg ggaaggtgga ggctgacatcccaggccatg2041 ggcaggaagt cctcatcagg taaaaggaag agattccatt gcccctgccacccacaccct2101 aagatcaagg gtgttcagct gcaaggtgga aagtttgcac gtggggtaggtcagttggct2161 gcattagtta agggtgttag aacggtcact tgctttttct ttgcttttaagtgtcaggga2221 ttggactcag gagagggaa

20、a ggagccattt caggctgatg tcagcagctggaggaagcat2281 gagaatcaaa cctaggatgc tcagagtcca ccaggaagaa ttttagaattatagacagtc2341 agagttaaca agggtcctga gagattttgt acagccacct ctcttacaggatgaggacaa2401 aaagcgactg agaaggggag gacatttcca gagtcacagc tcattaaatgctcttaaagt2461 gtcaaggtta agacatgctc ttcaagggga gacagatctg gttctagacttggctctgcc2521 actgagccac tgggtgacct ttgggaaggt acOfficial SymbolMBprovided by HGNCOfficial Full Namemyoglobinprovided by HGNCPrimary source :HGNC:6915Locus