Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemE(±)-EquolCat.No.:HY-100583ACASNo.:94105-90-5分?式:C??H??O?分?量:242.27作?靶點(diǎn):EstrogenReceptor/ERR;DrugMetabolite作?通路:Others;MetabolicEnzyme/Protease儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:≥100mg/mL(412.76mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM4.1276mL20.6381mL41.2763mL5mM0.8255mL4.1276mL8.2553mL10mM0.4128mL2.0638mL4.1276mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%saline1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESolubility:≥2.5mg/mL(10.32mM);Clearsolution2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(10.32mM);Clearsolution3.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(10.32mM);ClearsolutionBIOLOGICALACTIVITY?物活性(±)-Equolequol的外消旋體。(±)-Equol對(duì)ERα和ERβ的EC50sof分別為200和74nM。Equol??異酮?苷和??素的代謝產(chǎn)物。體外研究Equolisfirstisolatedandidentifiedfrompregnant-mares'urineandlaterfoundintheurineofthegoat,cow,henandsheep[1].Equol,unlikethesoyisoflavonesdaidzeinorgenistein,hasachiralcenterandthereforecanoccuras2distinctdiastereoisomers.S-equolistheexclusiveproductofhumanintestinalbacterialsynthesisfromsoyisoflavonesandbothenantiomersarebioavailable.S-equolhasahighaffinityforestrogenreceptorbeta(Ki=0.73nM),whereasR-equolisrelativelyinactive[2].EquolcouldpromotetheproliferationanddifferentiationofratosteoblaststhroughactivatingtheER-PKCα-relatedsignalingpathway.Thealkalinephosphataseactivityalsoincreasessignificantlyinalloftheequoland17β-estradiol(E2)groups.Equolalsosignificantlyelevatestheosteocalcinlevels[3].體內(nèi)研究Equolisamodestnatriureticandvasorelaxantagentintherat.Orallyadministeredequolisabout8-foldlesspotentthanorallyadministeredfurosemide.Inisolatedaorticringsprecontractedbyadministrationofphenylephrine,administrationofequolrelaxesthecontractedaorta(concentrationforhalf-maximalactivity58.9±16μM)[4].EquolpossessesanticanceractivitythatsuppressestumorformationviaapoptosisinductioninratswithDMBA-inducedmammaryglandtumors.Inaddition,equolshowsahepaticprotectiveeffectbyactingasanantioxidantandbyreducingapoptosis[5].PROTOCOLCellAssay[3]Primaryratosteoblastsaretreatedwith0.01-1μMequol,0.01-1μME2,or0.01-1μMequol/E2combinedwith1μMICI182780for24or48hours.Then10mL5mg/mLMTTsolutionisaddedtoeachwell.Theplatesareincubatedat37°Cfor4h,andthenthesupernatantisdiscardedand100mLDMSOisaddedtoeachwellandmixedthoroughlybeforetakingmeasurementsinamicroplatereader[3].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.AnimalRats:Equolorfurosemideisadministeredorallyatdosesof16,40,and100mg/kg(inavolumeof16mL/kgAdministration[3][5]5%arabicsyrup)togroupsof3-9rats(ratsofacontrolgroupareadministeredvehicleonly).Urinesamplesarecollectedfor6h.Urinarysodiumandpotassiumcontentsaremeasuredbyflamephotometry[3].Mouse:Equolisdissolvedinwaterandadministeredorallytoratsatadoseof5and25mg/kgBWfor8weeksafterasingledoseofDMBA(100mg/kg).Ascontrols,ratsaredividedintovehiclealoneandDMBAalonegroups.Inthesecondpart,ICRmiceareorallyadministeredequoldailyatadoseof5and25mg/kgBWfor7weeksbeforeasingledoseofDMBA(34mg/kg/week).Afterequoladministrationanimalsare2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEfollowedfor1weekcontinuously.Thecontrolgroupsarethesameasaboveandeachgrouparecomprisedofsixmice.Miceliversandmammaryglandtumorsareisolated,blotted,weighed,frozeninliquidnitrogenandstoredat-70°Cuntilassayed[5].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.REFERENCES[1].AxelsonM,etal.Theidentificationoftheweakoestrogenequol[7-hydroxy-3-(4'-hydroxyphenyl)chroman]inhumanurine.BiochemJ.1982Feb1;201(2):353-7.[2].SetchellKD,etal.S-equol,apotentligandforestrogenreceptorbeta,istheexclusiveenantiomericformofthesoyisoflavonemetaboliteproducedbyhumanintestinalbacterialflora.AmJClinNutr.2005May;81(5):1072-9.humanintestinalbacterialfl[3].WangJ,etal.Equolpromotesratosteoblastproliferationanddifferentiationthroughactivatingestrogenreceptor.GenetMolRes.2014Jul4;13(3):5055-63.[4].GimenezI,etal.Renalandvascularactionsofequolintherat.JHypertens.1997Nov;15(11):1303-8.[5].ChoiEJ,etal.Anticancermechanismofequolin7,12-dimethylbenz(a)anthracene-treatedanimals.IntJOncol.2011Sep;39(3):747-54.[6].CharoenthongtrakulS,etal.Enhancedgastrointestinalmotilitywithorallyactiveghrelinre