Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEGastrodinCat.No.:HY-N0115CASNo.:62499-27-8Synonyms:Gastrodine分?式:C??H??O?分?量:286.28作?靶點:Others作?通路:Others儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗DMSO:≥100mg/mL(349.31mM)H2O:100mg/mL(349.31mM;Needultrasonic)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制備儲備液1mM3.4931mL17.4654mL34.9308mL5mM0.6986mL3.4931mL6.9862mL10mM0.3493mL1.7465mL3.4931mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(8.73mM);Clearsolution2.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(8.73mM);Clearsolution3.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(8.73mM);Clearsolution4.請依序添加每種溶劑：PBSSolubility:100mg/mL(349.31mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY?物活性Gastrodin中草藥天?的主成分，具有抗炎作?。Gastrodin長期以來?直?于頭暈，癲癇，中風(fēng)和癡呆癥的研究。體外研究GastrodintreatmentreducesthemRNAexpressionlevelsofTNF-αandiNOSintheretinasofacuteocularhypertension[1].體內(nèi)研究IntraperitonealinjectionwithGastrodin10mg/kgor50mg/kgoncedailyfor15dsignificantlyinhibitsthelossofretinalganglioncells(RGCs)becauseofacuteocularhypertension(AOH)damage.2wkafterAOH,thenumberofIba1positiveretinalmicrogliaobviouslyreducesto231.3±54.3cells/mm2and201.9±43.1cells/mm2intheratsintraperitoneallyinjectedwithGastrodinat10mg/kgand50mg/kg,respectively[1].PROTOCOLCellAssay[1]Retinasarecollectedatday14postoperatively,followingtreatmentwithnormalsaline(NS)orvariousdosesofGastrodin.ThemRNAexpressionlevelsofTNF-αandiNOSaredeterminedbyreversetranscription-quantitativepolymerasechainreaction(RT-qPCR)[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.AnimalAdultfemaleSDrats(age,8wk;weight,200to250g)areused.Theratsaredividedinto4groups:1)controlAdministration[1]group;2)normalsaline(NS)groupwhoareexposedtoacuteocularhypertension(AOH)andreceiveintraperitonealinjectionof0.9%NS;3)G10groupwhoareexposedtoAOHandreceiveintraperitonealinjectionof10mg/kgGastrodin;4)G50groupwhoareexposedtoAOHandreceiveintraperitonealinjectionof50mg/kgGastrodin.Thelossofretinalganglioncells(RGCs)andthenumberofIba1-positiveretinamicrogliaaredeterminedat2wkafterrapidocularhypertension[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻?EurJPharmacol.2021Feb5;892:173734.?ImmunopharmacolImmunotoxicol.2022Jul26;1-10.2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE?ExpTherMed.2021Mar8.?NeurolInt.2022Jan20;1-8.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].WangJW,etal.Gastrodinprotectsretinalganglioncellsthroughinhibitingmicroglial-mediatedneuroinflammationinanacuteocularhypertensionmodel.IntJOphthalmol.2017Oct18;10(10):1483-1489.[2].JiangT,ChuJ,ChenH,etal.GastrodinInhibitsH2O2-InducedFerroptosisthroughItsAntioxidativeEffectinRatGliomaCellLineC6.BiolPharmBull.2020;43(3):480-487.McePdfHeightCaution:Prod