向GenBank批量上傳序列的方法Qin1利用DNAstar的editSeq制作待序列的FASTA文檔:待上傳序列刪減至合適長度后，將待上傳序列全部打開EdnS?qE由1?口Migvrvpthp<1EgtnfEdnS?qE由1?口Migvrvpthp<1EgtnfLMMtfiinh*M-irviiyp*1Eg*呻才田野中白d?ngu*?kllL]?二?EgtnvQt.stqEg?廿D；wqhih-:刖 甲M如卜―%gXWi愀版F中1一■H林!隨后點擊File-exportasone，保存文件格式為:*.fas將待上傳序列全部歸至一個FASTA文檔。注意序列命名：1405bpparti-記事本 —匚立杵舊編轉(zhuǎn)舊格式（5垂百M幫助的正氤「丁京”為名？需要工三城的白5匕R堂能M文!>Dengueviells1sirainGZ/ZT-&3/2014envelopeprotein（E）=snehpartialcds.seqHUS』ULij1tAA/AA1且UGL 11LG11AA3AL1U1LHbGAbLAAL1ItAAr1HUH1L4ULd11UjAAL區(qū)TGGAAGrrGCGrCACTACCATGGCAAAAGACAAACC-AACATTG^CATTG^ACTCITGAAGACGGAAGTCACAAACCCTGCCGTCCrGCGCAA.^CTGTGCAT7GAAGCTAAMTATCAMCACGYCCACCGACTCAAOTGTCCAACACAAGGAG^GCCACACTGGrGGUGUCAAGACGCGAArTTrGrGTGTCGACGAACGTTTGTGG^TAGL^KiCIGGGGCAATGGCTOrGGGCTCTTCGG^Ua5AAGCCTTATAACGTGTGCrMGTTCMGTGTGTGACm\TTGGAAGGAAAAATAGTTC.UTArGAAAACTTG^TArrCAGTMTTGTCACCGTCCACACTGGAGACCAGCACC.UGTGGGAA.\TGAAAGCACAG.UCATGGGACAACTGC.UCTATAACACCTC.UGCTCCCAC^CGGAAATACAGCTGACCGACTACGGAGCTCTTACACTGGATTOTrCACCTAGAACAGGACTAGACTTTAATGAAATGGTGTTGTTGACAATGAAAGAAAAATCATGGCTAGTCCACAAACAATGGTTTC7AGACCTACCACTGCCTTGGACCTCGGGAGCTTCA.^CATCACAAGA.AACTTGdUCAWCAAGATTTGCTGGraCATTTAAGACAGCTCATGCAAAGAAGCAGG.lAGTAGrCGTACTAGGATCAC^GWGGAGCA.MGCACACTGCGrr&-VCCGGAGCGACAG^TCCAAACGTCTGGAACGACAAC^ArTTTTGCAGGACATTTGUATGT'ACACTAAAGATGGACA.UCTGArTCTAAAAGGGATGrCATATGTGATGTGCACAGGCTCATTCAAGCTAGAGAAAGAAGT'GGCTGAGACCCAGCATGGAACCGTTCTAGTGCAGATTAAATACGAAGGAACAGATGCACCATGCAAGATTCCTTTTTCGiCCCAAGATGAAAAAGGAGTAACTCAGAATGGGAGATTGiTAACAGCCAACCCCATAGTTACTGACAAAGAAAAGCCAGTCAACATTGAGGCGGAACCGCCTTTTGGTGAGAGTTACATTGTGATnGGAGCAGGTGAMAA&CTTTGAAACTAAGCTGGTTCMGAMGGAAGTAGCATAGGG^ATGTTrGAGGCAACTGCCAGAGGAGCAC^GGATGGCCATACTGGGAGACACCGCATGGGi\CTTTGGTTCTATAGGAGGAGTGTTCACGTCTGnGGAAAATTAGTACACCAGATTTTTGGAACTGCATATGGGGTTTTGTTCAGCGGTGTTTCCrGGACCATG^UTAGGAATAGGGGTTCTGCTGACATGGCTAGGATTA.UCTC.UGG,^GCACGTCCCTTTCGATGACGTGCATT產(chǎn)GTTGGCCT3GTAACCCT3TKTnGGAGTWCGTTC3GGCG>Dengu&vims1strainGZ/ZT-fi2/2014envelopepratein（E）genc±partialcds.seqHJbLLrALbLLrl1AUULARLaLrALrALL1LLrI1bAAULielLJLrJLaiAxAiuL.^LA1IAAj1LrCrHJCrIbLrLALLA7GG.AAGCTGTGrCAC.CACCATGGCA.^tLUTAAACC.AACAT7GGACATTG^^CTCTTGAAGACGGAGGTCACGUCCCTGCCGTCTTGCGCAAACTGTGCATTGAAGCTAAAATATCAAACACCACCACCGATTCAA^TGTCCAACACAAGGAGWGCTACACTOTGGAAG/L^CAAGACGCGAACTTrGTGTGTCGACGAACATTCGrGGACA^&3CIGGGGTAATGGTTGTGG,^CTATTCGGGAAGGGUGCTTACTAACGTGTGCTAAGTTTAAGIGTGTGACAAAACTT^GGAAAGATAGTrCAArATGAAAACTTAAAATATTCGGTGATAGTCACTGTCCACACICKiGGACCAGCACCAGGrAGG^UTGAGACTACAGAACATGGUCAATTGCAACC&TAACACCTCAAGCTCCCACGECGGAAATACAGCTGACTGACTACGGAGCCCTTACaTTGCACTCCTCACCTAGAACAGGGCrGGACmAATGAGATGGTGCTGTTGACAATGAAAGAAAAATCArGGCTTGTCCACAAAC,UTGGrTTCTAGACTTACCArrACCTTGGACCTCGGGGGCrrCAACAlCTCAAGAGACTTGGAACAGACAAGATCTGCTGGTCACATTC^GACAGCTCArGCA/L4GA^GCAGG^GTAGTCGTACTGGGGTCrtCA1AG,UGG.i\GCAATGCACACTGCGrrG^rGGGGCGACAG.WTCCAG^CGTCAGGAACGACGACLWTCTT匚GC￡GGACACCTGAAATGTAGA匚TAAAAATGGATAAACTG4匚TT7AA.lAGGGGTGTCATATGTGATGTGCACAGGCrCATTTAAGCTAGAGAAGGAACTGGCTGAGACCCAGCATGGWCTGTCCTAGTGCAGGrr.\A.MATGAAGGAACAG^TGCACC.\TGCAAGAT7CC.UTTTCAACCCaUGATGAGA.lAGGAGTGACCCA.在GenBank的SubmissionTools里,下載序列信息編輯軟件Sequino:///Sequin/〔下列圖中點擊Instructions下載軟件〕HowtoGetSequinSequin13.70iscurrentlyavailablefromtheNCBI.SequinrunsonMacintosli,PC/Wir..dows>andUNIXcomputers.TnETrui:：cinsfordownloadingandinsT2.lltrigrheprogramareprovided.Yheprogramitalongwithitson__inehelpdocumentatioiinisavailablebyanonymousFTP.注意打開安裝文件后，程序會自動安裝在安裝包所在的文件夾。.打開Sequin，點擊StartNewSubmission。

4.隨后選擇序列發(fā)布日期，輸入論文題目。假設(shè)之前保存有模板，可以點擊下面的"Clickheretoimportatemplate"導入。模板導出在下文的第五點提到。SubmittingAuthors

.聯(lián)系人、作者信息、機構(gòu)或者學校信息SubmittingAuthorsSubmittingAuthorsFileEdit5ubniss：iun11^Contact'[[AuthorsIfftitiliationConsortium5ubniss：iun11^Contact'[[AuthorsIfftitiliationConsortiumTaeu_i;ii：LxLiiLJii[_eli_Lil.uuLilLeuatd au_i；ii：LxLiiui.isreaponsiblefortLiesequentingorpub1icationnEthedata..Individualauthorsnia.ybelistedalong￥ithacensortiunname.<<F'revPageHqxt.Page>><<F'revPageHqxt.Page>>SubmittingAuthors建議導出模板，之后修改信息時可以直接導入模板而不需重新輸入。.選擇上傳的序列類型。PreparingtheSequencesHovdoyciuvanttopreinareyoursubmission?CUsethenormalsubmissiondialogUseaSubmissionwizard:UnculturedSamplesCUnculturedSamplesCrRNA-ITS-IGSsequencesIntergenicSpacer(ICS)gequencesNicrc-satBllitBsequencesD-loopsandcontrolregionsBackNext.導入之前準備好的FASTA文件:WizardImiportNucleoiideSequencesWizardImiportNucleoiideSequences導入之后，輸入序列ID〔序號ID相當于編號，可以自由命名，只要各個序列的ID不一致即可。在這里以病人編號作為序列ID〕ProvideSequenceID?ForYourSequences.iCO5dfRUTEEUBI1C曰IN3EdHplICSt-edFIimslprevids-Emiqiie5tqierio="Ifetor臺亡：r?sequriDceYdi.tfwqiwnw工工0-are-not .工冗日Terntrytojmtspace*Ftc：iti.an. SuErgest?dID 0rigin2)l ED1 I』Emmirws 1』即受Z DaHgoEl工整s BaHgnjs3 Oeogmr工助s Befigrijs? DefigoElrws Defigus5 BetigiiK'iivis Beugiif6 DeiiguM'lx-us teiigiieint?-5?rrwflELD&?Thisi&力口t?LJc*wdOriginalTitlevix-HS1jstrm篦GTST督111glopepreteitj[Ej*hclparti?dcdo.sequlXM"1straiiaGE^ZS-SZ^O]a<sn.?slDpsprotedH[ElpftrtlaiL匚do.弓占口1istrisiaGUZF口iKNUllsa^wlDpejee口teiti￡EJ9eHe=psitlail口djffi.ajcq.vlrw1ctriLlAra-/ZB-5 4-11ylLqj?prot?Lu[E]getue.partialcdL.seqvliB&11tj.axiiGZ『LV-su'rtlope-ju'onslti[E]9M1*.gMiUcds.seqviiTis：3stta^LriGZ/ZV-3Z^ID]4efi'JtlopeproteLti[E]ClearyiiedIitotsEefresh.ErrorLisiAut.oMrrectS&^rKieIBsExtot PDsiticnSequuQIDTitlffi*,.Ddpliat口工口1Dupl ID 2Duplic-itrLD3.DupliwtrID4Dghei-k-virus1strainCZ/Zf-^3/2Q14edvqLqpbvratcin(I)bbob,pirtialqje.shq.virus1swisixiGZ/Zf-02/2014-Ai'ivc-L^pdipxatcd.nCl)gn,paxtidLcdsi.i口用[im?gviruB1istaiiJiGZ/ZI_55/^014boveIm石jiroiein(Dtseos.psrtn5lcds^raeq.Dencue-*irua1strainGZ/Zf-S4^Ol-imrelqpeprotein?u曰k,partialoJs.3EQ.*sn句Y爾Lueocesritherx^rBC冊二“1%1L駕E」luk員與mret“由iCam式ID?ForYqufSequences輸入序列序號后「系統(tǒng)提示己更正，點擊dCEptCl日仃Cl日仃yixedIrr=：iT3Eefresh.ErrorList士|所isdvirus1strainCZ/Z>-：L9/2ul-4-crn'c-lopsprotctin(I)Ecna,partialWe.mq.Fixed 9nivlriis]striiSxiCZ/Zf-L14/ZD：L4SKglQpaprciUm1：口￡弗/pwiisd.eda.aaqPix^d 1076￥iruE11511m口GZ/ZI_7iiiJSi]l+erft!el<rpejircstedn(DTens.psrt-iiLcds.?eq.~|Fi?ad. 1116virin13trainGZ/Zf~LB/2014snclqpEjuutein?use,partialojs.3EQ.Sei?-Titis-ShwwilrsequencK：rithcttoteC出口?ill5?jJBrKBi5insrt匕心已中tIC口mulI導入序列成功后，顯示各個序列信息如下:1143617578921771879579WizardImportNucleotideSequencesFileEdit|Sequences.SequencingMethodFileEdit|Sequences.SequencingMethodsequences

11185nt

148Ent

1485nt

1405nt

sequences

11185nt

148Ent

1485nt

1405nt

1485nt14B5ntlaBEntUBSnt14S5nt14GGnt1465ntOrtitmalAJ：r.Enner.LSetLinasClearNuclBotLdeFASTAVectorTriOrtitmalAJ：r.Enner.LSetLinasClearNuclBotLdeFASTAVectorTrinToedEackHeitInportNucleoticeFASTIFASTAFornatHeLo點擊SequencingMethod，將測序方法輸入:WizardImiportNucleoiideSequencesWizardImiportNucleoiideSequences選擇上傳序列的用途:SubmissianiTypeLtypa口EluhaizKinn￡■LliiK?"Pqpset:PocmlatimS-tu4r：a.setofssdiehedttstteeedErimlt?y9eqjEna.ncthesue±r?idLifereEiti9ala.tE9ofthesuewraoimL# Stuily:■mtqEbbqmncBBttwbJajif肩byEcquBtKingibd日mrBeoaditfrr?nt-grrmiBJB.Kutsst;llrtatlcinStudy:白窩鼠E融色詢ice*thatferedEfl?ed11y亭后。座腑EjaultlplenuUtlswEaKt帽3aeiie：-E-Qtdn.:Duekjtprxsqaiasat.囪aHst|病毒類型:VirusWizardTypeofVirusAreallofyoursequencesfromanyofthe：療巳viruses?CNorDvirus,Sapovirus(Caliciviridae)rFoot-and-nouthdiseaseTirusCInfluenzavirusCRotaviruig?日口tlistedaboveormixedsetofdifferentvirusedBacktlsxt.編輯每條序列的信息:點擊ImportSourceTable可以導入模板，點擊ExportThisTable可以輸出模板備用。.選擇核酸類型，序列內(nèi)容后，點擊OpenRecordVieweroVirusWizardMoleculeInformationWnatjftjleculetvziewasiszilatedf'enonicRW|LinearBackNext

BackNextVirusWizardAnnotation(Jhatdoyoursequencesnz：ontain'?CSinglecodingregionacrosstheentiresequenceCVirusWizardAnnotation(Jhatdoyoursequencesnz：ontain'?CSinglecodingregionacrosstheentiresequenceCSinglenon-codingfAatureacrosstheentiresequence(iMuitiplefeattirsgpersequence(codingregionSjLTRs.,etc,EackHextVirusWizardAnnotationIncompleteat5,InconpleteatS3日n匚CodingregionisonminusstrandEackNext「duwillnovbetransferredtotherecordvievrer.AddannotationnjiiigtheiLenuoptionsorafeaturetable.Ycannotrst.LirntothevizardonceyouopentheTecc-rdviewer.ClickCanceltoccmtinueeditingyouiinformationintheYidzard^OpenRecordVie^verCancel.添加每條序列的信息，包括編碼區(qū)重復區(qū)RNA結(jié)構(gòu)等等。雙擊相關(guān)信息可進行修改，點擊Annotate添加相關(guān)信息。

11檢查信息是否有誤〔也可以點擊Search里的Validate檢測是否有明顯錯誤〕：有些錯誤是可以忽略的，如上傳的序列為CDS序列，那么軟件報錯”序列無終止子”時可忽略。隨后，點擊Done保存待上傳序列：注意此時保存的文件包含所有序列信息，因此只需保存一次即可。

12.將保存好的文件作為郵件附件，發(fā)送至：gb-sub@flewSubmissionaboutDenguevirusIEgene發(fā)件人：X.<.12.將保存好的文件作為郵件附件，發(fā)送至：gb-sub@flewSubmissionaboutDenguevirusIEgene發(fā)件人：X.<.colin666666@>I時間：2018年了月習(星期五】晚上11:32:gb-sub'ib-subg>ncbtnliTLnihL!.DenguevirustFgeneisolateDENV-1-GZ-ZW-ZS3-XX-201.4.sqn)DearGenBankstaff:HereIsoursubmfsslonaboutEgeneofdengue