AbMole科研小講堂丨Hepcidin-25：連接炎癥、貧血與腫瘤的關(guān)鍵生物標(biāo)志與研究工具Hepcidin-25是一種由25個(gè)氨基酸組成的肽類激素，在其肽鏈中存在著一條金屬結(jié)合基序，并在脊椎動(dòng)物鐵代謝調(diào)控中發(fā)揮核心作用ADDINEN.CITEADDINEN.CITE.DATA1。作為鐵穩(wěn)態(tài)的"主調(diào)控因子"，它通過抑制腸道鐵吸收和巨噬細(xì)胞鐵釋放來調(diào)控循環(huán)鐵的水平ADDINEN.CITEADDINEN.CITE.DATA2。Hepcidin-25常常作為疾病模型中的分析指標(biāo)或者生物標(biāo)志分子。例如在健康狀態(tài)下，大鼠血漿中Hepcidin-25的平均濃度為63.0ng/mL，而在脂多糖刺激后顯著升高至218.2ng/mL，表明Hepcidin-25與炎癥反應(yīng)高度相關(guān)ADDINEN.CITEADDINEN.CITE.DATA3。在心血管領(lǐng)域，研究發(fā)現(xiàn)Hepcidin-25與動(dòng)脈僵硬度存在關(guān)聯(lián)ADDINEN.CITEADDINEN.CITE.DATA4。Hepcidin-25還可調(diào)節(jié)鐵代謝，Hepcidin-25與其受體鐵輸出蛋白結(jié)合后，可抑制腸道鐵吸收以及肝細(xì)胞和巨噬細(xì)胞的鐵外排。檢測(cè)Hepcidin-25可以預(yù)測(cè)機(jī)體的鐵代謝狀態(tài)，并有助于研究貧血和某些以貧血為特征的動(dòng)物疾病模型，例如慢性腎臟病（CKD）、糖尿病相關(guān)的慢性腎病、炎癥性腸病血色病等動(dòng)物模型ADDINEN.CITE<EndNote><Cite><Author>Ueda</Author><Year>2017</Year><RecNum>1502</RecNum><DisplayText><styleface="superscript">5</style></DisplayText><record><rec-number>1502</rec-number><foreign-keys><keyapp="EN"db-id="f2td9w00a22awteprfrp9vaup9d9zwa9tdfr"timestamp="1764642425">1502</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author>Ueda,Norishi</author><author>Takasawa,Kazuya%JCurrentmedicinalchemistry</author></authors></contributors><titles><title>Roleofhepcidin-25inchronickidneydisease:anemiaandbeyond</title></titles><pages>1417-1452</pages><volume>24</volume><number>14</number><dates><year>2017</year></dates><isbn>0929-8673</isbn><urls></urls></record></Cite></EndNote>5。Hepcidin-25的升高還可用于監(jiān)測(cè)腫瘤的發(fā)生發(fā)展，例如Hepcidin-25與肝細(xì)胞癌的腎臟轉(zhuǎn)移有關(guān)ADDINEN.CITE<EndNote><Cite><Author>Kamai</Author><Year>2009</Year><RecNum>1504</RecNum><DisplayText><styleface="superscript">6</style></DisplayText><record><rec-number>1504</rec-number><foreign-keys><keyapp="EN"db-id="f2td9w00a22awteprfrp9vaup9d9zwa9tdfr"timestamp="1764645559">1504</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author>Kamai,Takao</author><author>Tomosugi,Naohisa</author><author>Abe,Hideyuki</author><author>Arai,Kyoko</author><author>Yoshida,Ken-Ichiro%JBMCcancer</author></authors></contributors><titles><title>Increasedserumhepcidin-25levelandincreasedtumorexpressionofhepcidinmRNAareassociatedwithmetastasisofrenalcellcarcinoma</title></titles><pages>270</pages><volume>9</volume><number>1</number><dates><year>2009</year></dates><isbn>1471-2407</isbn><urls></urls></record></Cite></EndNote>6，并且Hepcidin-25還可作為非侵入性乳腺癌預(yù)測(cè)工具。目前針對(duì)Hepcidin-25的檢測(cè)手段包括LC-MS/MS法、ELISA檢測(cè)試劑盒等技術(shù)。Hepcidin-25還具有其它的生物學(xué)應(yīng)用例如抗菌，有研究表明Hepcidin-25的水平在細(xì)菌感染后會(huì)明顯上調(diào)，表明其與機(jī)體的主動(dòng)防御有關(guān)。而在體外實(shí)驗(yàn)中，Hepcidin-25及其衍生物Hepcidin-20對(duì)多種病原微生物表現(xiàn)出抑制活性ADDINEN.CITE<EndNote><Cite><Author>Maisetta</Author><Year>2010</Year><RecNum>1505</RecNum><DisplayText><styleface="superscript">7</style></DisplayText><record><rec-number>1505</rec-number><foreign-keys><keyapp="EN"db-id="f2td9w00a22awteprfrp9vaup9d9zwa9tdfr"timestamp="1764647141">1505</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author>Maisetta,Giuseppantonio</author><author>Petruzzelli,Raffaele</author><author>Brancatisano,FrancaLisa</author><author>Esin,Semih</author><author>Vitali,Alberto</author><author>Campa,Mario</author><author>Batoni,Giovanna%JPeptides</author></authors></contributors><titles><title>Antimicrobialactivityofhumanhepcidin20and25againstclinicallyrelevantbacterialstrains:effectofcopperandacidicpH</title></titles><pages>1995-2002</pages><volume>31</volume><number>11</number><dates><year>2010</year></dates><isbn>0196-9781</isbn><urls></urls></record></Cite></EndNote>7。Hepcidin-25和Hepcidin-20對(duì)多藥耐藥性細(xì)菌的抑制濃度ADDINEN.CITE<EndNote><Cite><Author>Maisetta</Author><Year>2010</Year><RecNum>1505</RecNum><DisplayText><styleface="superscript">7</style></DisplayText><record><rec-number>1505</rec-number><foreign-keys><keyapp="EN"db-id="f2td9w00a22awteprfrp9vaup9d9zwa9tdfr"timestamp="1764647141">1505</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author>Maisetta,Giuseppantonio</author><author>Petruzzelli,Raffaele</author><author>Brancatisano,FrancaLisa</author><author>Esin,Semih</author><author>Vitali,Alberto</author><author>Campa,Mario</author><author>Batoni,Giovanna%JPeptides</author></authors></contributors><titles><title>Antimicrobialactivityofhumanhepcidin20and25againstclinicallyrelevantbacterialstrains:effectofcopperandacidicpH</title></titles><pages>1995-2002</pages><volume>31</volume><number>11</number><dates><year>2010</year></dates><isbn>0196-9781</isbn><urls></urls></record></Cite></EndNote>7參考文獻(xiàn)及鳴謝ADDINEN.REFLIST(1)Mead,M.K.;Claus,M.;Litton,E.;Smart,L.;Raisis,A.;Rossi,G.;Trengove,R.D.;Gummer,J.P.A.IdentificationofTheCanidaeIronRegulatoryHormoneHepcidin.Scientificreports2019,9(1),19400.DOI:10.1038/s41598-019-55009-wFromNLM.(2)Kesharwani,P.;Dash,D.;Koiri,R.K.Decipheringtheroleofhepcidininironmetabolismandanemiamanagement.Journaloftraceelementsinmedicineandbiology:organoftheSocietyforMineralsandTraceElements(GMS)2025,87,127591.DOI:10.1016/j.jtemb.2025.127591FromNLM.(3)Sakamoto,S.;Kirinashizawa,M.;Mohara,Y.;Watanabe,Y.Generationandcharacterizationofmonoclonalantibodiesagainstmaturehepcidinanditsapplicationtoneutralizationandquantitativealterationassay.Bioscience,biotechnology,andbiochemistry2021,85(2),340-350.DOI:10.1093/bbb/zbaa013FromNLM.(4)Wang,X.;Sheng,L.;Ye,P.;Cao,R.;Yang,X.;Xiao,W.;Zhang,Y.;Bai,Y.;Wu,H.TheassociationbetweenHepcidinandarterialstiffnessinacommunity-dwellingpopulation.Lipidsinhealthanddisease2018,17(1),244.DOI:10.1186/s12944-018-0866-6F