注：不含主觀題第1題單選題(10分)Whichofthefollowingisnota“Watson–Crick”pair()AA-T;BU-G;CU-A;DC-G第2題單選題(10分)Whichofthefollowingaminoacidsismosthydrophobic()AA;BS;CE;DF第3題單選題(10分)WhichofthefollowingisthetypicalstructureofaDNAmolecule()ASinglestrand;BSinglecircle;Cdoublehelix;Ddoublesheets第4題單選題(10分)WhichofthefollowingisthecorrectdescriptionaboutCENTRALDOGMA()ARNAsynthesisisdirectedbyaDNAtemplate;BDNAsynthesiscanneverbedirectedbyanRNAtemplate;CProteinsynthesiscanbedirectedeitherbyRNAorDNA;DDNAsynthesiscanbedirectedbyaproteintemplate第5題單選題(10分)WhofirstprovethatDNAisthegeneticinformationcarrier()AMendelBOswaldCWatson&CrickDMauriceWilkinsandRosalindFranklinEAveryandhiscolleagues第6題判斷題(10分)Anucleosideisformedthroughaphosphodiesterbondbetweenthehydroxylonthe1’carbonofthesugarandthebase.()第7題判斷題(10分)ThedoublehelixisstabilizedbybasepairingandbaseStacking.()第8題填空題(10分)Mendel’s

firstlaw:

____

正確答案：:["Thevarioustraitsarecontrolledbypairsoffactors(whichwenowcallgenes),onefactorderivedfromthemaleparent,theotherfromthefemale","不同的性狀由成對(duì)的因素(我們現(xiàn)在稱之為基因)控制,一個(gè)因素來自父本,另一個(gè)來自母本"]第9題填空題(10分)Mendel’s

secondlaw:____正確答案：:["?Eachgenepairwasindependentlytransmittedtothegameteduringsex-cellformation","在性細(xì)胞形成過程中,每對(duì)基因都獨(dú)立地傳遞給配子"]第10題填空題(10分)____isthetemplateforproteintranslation正確答案：:["RNA"]Unit2Quiz第1題單選題(20分)ThedirectionofDNAreplicationis:Aleadingstrand5’to3’,laggingstrand5’to3’Bleadingstrand5’to3’,laggingstrand3’to5’Cleadingstrand3’to5’,laggingstrand5’to3’Dleadingstrand3’to5’,laggingstrand3’to5’第2題單選題(20分)WhichDNApolymeraseisrequiredforbacterialDNAreplication?APolIBPolIICPolIIIDAlltheabove第3題單選題(20分)WhichproteinisrequiredforDNAreplication?AhelicaseBtopoisomeraseCsingle-strandedbindingproteinDalltheabove第4題單選題(20分)PolIIIcorecanreplicateDNAatabout____

nt/secinvitroA1000B730C1D5000第5題單選題(20分)Mammalsuse____

torepairpyrimidinedimersAPhotolyaseBNonhomologousEnd-JoiningCBaseExcisionRepairDNucleotideExcisionRepairUnit3Quiz第1題填空題(20分)PrimosomeinE.coliiscomposedofDNAhelicase____andprimase____正確答案：:["DnaB"]正確答案：:["DnaG"]第2題判斷題(20分)PolIIIcorecanreplicateDNAprocessivelyatabout1,000nt/secinvivo.第3題填空題(20分)____structurecanprotectchromosomefromnucleasesanddoublestrainbreakrepairenzymes.正確答案：:["Telomere"]第4題填空題(20分)Loadingandunloadingbeta-subunitareperformedby____subunit正確答案：:["gamma"]第5題填空題(20分)____

subunitactsastheclampandslidesofftheendsofalinearDNAineukaryotes.正確答案：:["beta"]Unit4Quiz第1題單選題(10分)ThefunctionofSpo11is:()ASpo11isarecombinasethatbindsthenewlycreatedsingle-strandregion;BSpo11cutstheDNAandformsaDNAdoublebreak;CSpo11isasinglestrandDNAbindingproteinandassemblesthestrand-exchangeproteinfilaments;DNoneofabove.第2題單選題(10分)Whichofthefollowingpairsofproteinshavethesimilarfunctions:()ARecBCD-MRX;BRad51-Dcm1;CRecA-Rad51;DAllofabove.第3題單選題(10分)WhichofthefollowingstatementsisTrue?()ARecombinationbetweentwodirectrecombinationsitesinthesameDNAmoleculewillgenerateadeletionproduction;BRecombinationbetweentwoinvertedrecombinationsitesinthesameDNAmoleculewillgenerateadeletionproduction;CRecombinationbetweentwodirectrecombinationsitesinthetwoDNAmoleculeswillgenerateadeletionproduction;DRecombinationbetweentwodirectrecombinationsitesinthesameDNAmoleculewillgenerateaninsertionproduction.第4題多選題(2分)InV()Jrecombination,therecombinationwillhappeninwhichofthefollowingsites:()ACGCAGTG----12-bpspace----ACAAAAACA/CGCAGTG----12-bpspace----ACAAAAACA;BCGCAGTG----23-bpspace----ACAAAAACA/CGCAGTG----23-bpspace----ACAAAAACA;CCGCAGTG----23-bpspace----ACAAAAACA/CGCAGTG----12-bpspace----ACAAAAACA;DAllofabove.正確答案：C第5題判斷題(10分)TheRecBCDcomplexhasbothnucleaseandhelicaseactivity,andrequiresATP.()第6題判斷題(1分)RecAandSSBcanbothbindsingle-strandDNAs,sotheycanreplaceeachotherinhomologuerecombination.()第7題判斷題(10分)PhageCreandyeastFLPbothbelongtotyrosinerecombinasefamily.()第8題單選題(1分)WhichoneofthefollowingstatementsaboutDNArecombinationisWRONG?()ADNArecombinationcanrepairthedamagedDNA;BDNArecombinationgeneratesgeneticvariations;CDNArecombinationcanhelprestartstalledreplicationforks;DDNArecombinationisalwaysinitiatedbyDNAdamages.第9題單選題(1分)Theproperorderofhomologuerecombinationis:()(1)DNAbreak(2)Resolution(3)Alignment(4)Strandinvasion(5)FormationoftheHollidayjucntionA4-5-3-1-2;B2-4-5-3-1;C3-1-4-5-2;D1-4-3-2-5.第10題單選題(1分)WhichofthefollowingproteinresolvestheHollidayjunction?()ARuvAB;BRuvC;CRecC;DRecB.Unit5Quiz第1題判斷題(20分)Lacoperon,araoperonandtrpoperon,allofthemwilltranscribeapolycistronicmRNA.第2題單選題(20分)TurningonthelacOperonwillpermitthebacteriatousethesugarAGlucoseBLactoseCBothofthemDNoneofthem第3題填空題(20分)Twomaintypesofterminatorsare____terminatorand____terminator.正確答案：:["rho-dependent"]正確答案：:["rho-independent"]第4題填空題(20分)____subunitofRNApolymeraseplaystheroleinUPElementRecognition.正確答案：:["alpha"]第5題填空題(20分)____subunitofRNApolymerasestimulatesinitiationoftranscription.正確答案：:["sigma"]Unit6Quiz第1題填空題(20分)

Eukaryotictranscriptionfactorsinclude____factorsand____factors.正確答案：:["generaltranscription"]正確答案：:["gene-specifictranscription"]第2題填空題(20分)Phosphorylationinserine____ofpolIICTDindicatesthetranscriptioninitiationandserine____indicatesthetranscriptionelongation.正確答案：:["5"]正確答案：:["2"]第3題判斷題(20分)AllthepromoterelementslocateupstreamoftheTSS.第4題判斷題(20分)AllthepromotersinEukaryotescontainTATAbox.第5題填空題(20分)RNAPolymerase____isresponsibleforthetranscriptionofmostmRNAs.正確答案：:["II"]Unit7Quiz第1題填空題(20分)____isthemethodtodetectDNAlooping.正確答案：:["3C"]第2題判斷題(20分)Chromatinstructurealsocontrolsthegeneexpression.第3題填空題(20分)WhichhistonesexistinCorehistones?____,____,____and____正確答案：:["H2A"]正確答案：:["H2B"]正確答案：:["H3"]正確答案：:["H4"]第4題判斷題(20分)Thefunctionaldomainsofactivatorsareindependentmodules.第5題填空題(20分)Whatarethetwofunctionaldomainsof

activators?____and____正確答案：:["DNA-bindingdomain"]正確答案：:["Transcription-activationdomain"]Unit8Quiz第1題單選題(10分)Exon/()-intron-()/Exonistheconsensussequencesintheboundaryofclassicalintrons.()AGUAG;BGAUG;CAUGA;DAGGU第2題單選題(10分)Spliceosomecontains()AsiRNA;BmiRNA;ChnRNA;DsnoRNA第3題單選題(10分)TheU2snRNPcanbindwith()A3’-splicesiteofpre-mRNA;B5’-splicesiteofpre-mRNA;Cexon1ofpre-mRNA;Dbranchpointofpre-mRNA第4題單選題(10分)Aminorsplicesomecontains()AU1,U2,U4andU6snRNPs;BU1,U2,andU4atacsnRNPs;CU11,U12,andU5snRNPs;DU11,U12,U4andU5snRNPs第5題單選題(10分)Thefirststepofspliceosomeassemblyis()AU1snRNAbindsto5’-ssofpre-mRNA;BU2snRNAbindstoU1snRNA;CU5snRNAbindsto3’-ssofpre-mRNA;DU2snRNAbindsto5’-ssofpre-mRNA第6題單選題(10分)WhichoneisacorrectdescriptionabouttherelationshipofRNApolIIandsplicingregulation:()ARNApolIIRpb1subunitN-terminaldomainbindstothesplicingfactors;BRNApolIIoftensuppressesexonsplicing;CRNApolIIregulatessplicingefficiencyaftertranscriptiontermination;DRNApolIIinfluencesplicingefficiencywithexondefinition第7題單選題(10分)WhichoneisNotacorrectdescriptionaboutalternativesplicing:()AAlternativesplicingisaneffectivewaytoincreaseproteindiversity;BAlternativesplicingcascadeofSxl-tra-dsxdeterminesmousegenderselection;CAlternativesplicingpatterninthemouseimmunoglobulinμheavy-chaingenedeterminessecretioncharacteristicsofthesplicingproducts;DSometimesSRproteinsareimportantsplicingfactorsfordifferentpre-mRNAs第8題判斷題(10分)Self-splicingofgroupIintronbeginswithattackbyaguaninenucleotideonthe5'-splicesite(

)第9題判斷題(10分)BothgroupIandgroupIIintronsareself-splicingRNAs()第10題判斷題(10分)pre-mRNAsplicingmayhaveevolvedfromgroupIintrons,duetothesimilarcatalyticmechanismaswellasthestructuralsimilarityofsplicingmachineryandGroupIIintrons()Unit9Quiz第1題單選題(10分)Ifthefirsttwonucleotidesatthe5’-endofanuncappedmRNAispppXpY,whatwillitbeaftercapping?（）Am7Gpp;Bm6Gpp;Cm7Gppp;Dm6Gppp第2題單選題(10分)WhatarearethechargesofXpYandXpifnottoconsiderthechargesofXandY?(X&Yrepresentanynucleotide,prepresentsphosphategroup)()A0,+1;B-1,-2;C0,-1;D-2,-1第3題單選題(10分)mammalianpolyadenylationsignalis()AAUUAAA;BAUAAA;CAAUAAA;DAAAUAA第4題單選題(10分)CPSFbindsto()AAGrichmotif;BAUrichmotif;CGUrichmotif;DCUrichmotif第5題單選題(10分)Whichoneofthefollowingeventsdoesnottakeplaceduringtranscription()ASplicing;BCapping;CPolyadenylation;Dm6AMethylation第6題單選題(10分)WhichoneofthefollowingdoesnotthetruemRNAmethylationform:()Am6G;Bm7G;Cm5C;Dm6Am第7題單選題(10分)WhichoneofthefollowingisNotacorrectdescriptionaboutthecoordinationofRNAprocessingevents:()AthecoordinationofRNAprocessingeventsreliesonthepresenceofRNApolymerase;BRpb1CTDSer-2phosphorylationfacilitatesmRNAcapping;CRpb1C-terminaldomainactasaplatformforallthreemRNAprocessingevents;DRpb1CTDcanbephosphorylatedonSer-2,5&7第8題判斷題(10分)RNApolyadenylationneedsparticipationofRNApolymeraseIIIandpolyApolymerase()第9題判斷題(10分)MessegnerRNAcanbemethylatedincodingregion()第10題判斷題(10分)Transcriptionterminationcompletelydoesnotneedtheexistenceofpolyadenylationsite()Unit10Quiz第1題單選題(10分)WhichofthefollowingsbelongtosmallnoncodingRNAs?()AtRNA;BrRNA;C5’-UTR;DpiRNA第2題單選題(10分)WhichofthefollowingsdoesnotbelongtotheRNAeditingevents?()AUdeletion;BUinsertion;CconversionfromCtoI;DconversionfromAtoI第3題單選題(10分)WhichofthefollowingproteinsresponsibletoamplifymoresiRNAsindsRNAtransfectedcells?()ADICER;BRDRP;CRISC;DRITS第4題單選題(10分)WhatistheproteincomplextocleavetargetmRNAwhichcancomplementwithsiRNA()ADICER;BRDRP;CRISC;DRITS第5題單選題(10分)WhichoneofthefollowingcaninteractwithGW182:()AAGO1;BAGO2;CAGO3;DAGO4第6題單選題(10分)WherethemRNAsisdegraded?()AP-bodies;Bexosome;Cautophagosome;DGolgibodies第7題判斷題(10分)siRNAwasproducedfromendogenousprecursorlongdsRNA.()第8題判斷題(10分)BothofmiRNAandsiRNAcansuppressproteintranslation.()第9題判斷題(10分)RNAinterferenceisadefensemechanismthatprotectsagainstvirusesandtransposons.()第10題填空題(10分)Themechanismcausingsilencingofapurplecolorgeneinpetuniabyaddingextracopiesofthecolorgeneiscalled____正確答案：:["RNAinterference","RNAi"]Unit11Quiz第1題單選題(10分)CurrentlywhichoneofthefollowingsisprovedtobeabletoactasmiRNAsponges?()AlncRNA;BcircRNA;CpiRNA;DCRISPRRNA第2題單選題(10分)WhichofthefollowingdescriptionsisNOTcorrectaboutCRISPR-Cassystem?()AThereare6typesofCRISPRRNAs;BThenaturaltargetsofCRISPR-Cas9systemareDNAsandRNAs;CCRISPR-CAS13canonlytargetRNA;DTheCRISPRsysteminEcoliisdifferentwiththeCRIPPsysteminFfuriosus.第3題單選題(10分)WhichofthefollowingdescriptionsisNOTcorrectaboutXIST?()AXISTtranscriptscanbedetectedinbothofmaleandfemalesamples;BXISTcoatinginducesDNAmethylationinX-chromosome;CXistspreadingisLINEdependent;DXchromosomeinactivationleadstotheformationofBarrbody.第4題判斷題(10分)SomecircRNAshaveprotein-codingcapacityandcanencodeproteins()第5題判斷題(10分)piRNAclusterscanbefoundinsomaticcells.()第6題判斷題(10分)ACRISPRRNAcontainsalocusofCRISPRoperonandalocusofCRISPRarray.()第7題判斷題(10分)Agonaute3(Ago3)participatesintopiRNAping-pongamplificationloop.第8題填空題(10分)TelomeraseRNAbelongsto____RNA.正確答案：:["Inc"]第9題填空題(10分)LncRNAcanbeclassifiedinto2types:

____and

____正確答案：:["cis-actinglncRNAs"]正確答案：:["trans-actinglncRNAs"]第10題Unit12Quiz第1題單選題(1分)TherearethreetRNAbindingsitesontheribosome,whichofthefollowingcanbindaminoacyl-tRNA?

AAsiteonly;

BPsiteonly;

C

Esiteonly;

DAandPsites;

EA,PandEsite.第2題單選題(1分)Whichofthefollowingstatementsaboutthegeneticcodeisincorrect?()AThegeneticcodeismadeupwith3bases;BThecodingmessagemaycontainsomegaps;CThecodonsarereadina5‘to3’direction;DThegeneticcodesarenonoverlapping.第3題單選題(1分)Whichofthefollowingdoesnotbelongtoaeukaryoticribosomelargesubunit?()A23SrRNA;B28SrRNA;C5SrRNA;D5.8SrRNA.第4題單選題(1分)Whichofthefollowingtrinucleotidemaycausephenylalanyl-tRNAtobindtheribosome?()AUUA;BUCA;CUUC;DUGC.第5題判斷題(1分)Accordingtothewobblehypothesis,thethirdpositionoftheanticodonisthewobbleposition.()第6題判斷題(1分)ThepeptidyltransferasecenterandthedecodingcenterarecomposedeitherentirelyormostlyfromRNA.()第7題單選題(1分)TheshortsequenceupstreamofthestartcodeinprokaryoticmRNAiscalled:()AKozaksequence;BPromoter;CEnhancer;DShine-Dalgarnosequence第8題單選題(1分)Whichofthefollowingisnotusedasastopcode?()AUGA;BUAG;CUGG;DUAA第9題單選題(1分)WhatareTheinteractionsthatstabilizethetRNAternarystructureinclude:()AInteractionbetweenthebasesandthesugar-phosphateBBase-stackinginteractionCHydrogenbondsDAllofabove第10題單選題(1分)HowmanytRNAmoleculesarerequiredtodecodetheuniversalcodeaccordingtothewobblerules:()A22;B28;C32;DUnit13Quiz第1題單選題(1分)Whichofthefollowingcontributetothespecificityoftranslation?()Aadditionalhydrogenbondsbetweentwoadenineresiduesofthe16SrRNAandtheminorgrooveoftheanticodon–codonpaircanbeformedwhenthefirsttwobasesoftheanticodon–codonpairformcorrectWatson–Crickbasepairs;Bcorrectcodon–anticodonbasepairingfacilitatesEF-Tuboundtotheaminoacyl-tRNAtointeractwiththefactor-bindingcenterinducingGTPhydrolysisandEF-Turelease;Conlycorrectlybase-pairedaminoacyl-tRNAsremainassociatedwiththeribosomeastheyrotateintothecorrectpositionforpeptide-bondformation.DAllofabove.第2題單選題(1分)Thepeptide-bondformationduringtranslationiscatalyzedby().A16SrRNA;B23SrRNA;CL2;DL22第3題單選題(1分)Afterpeptide-bondformation,thepeptidyl-tRNAisinhybridstate.()AE/P;BE/A;CP/A;DNoneofabove.第4題單選題(1分)TheproteinfactorEF-Tsfunctionsas().AGEF;BGAP;CGTPase;DNoneofabove.Answer第5題判斷題(1分)Aspecialaminoacyl-tRNAsynthetaseaddedtheformyl-methioninetotheinitiatortRNAinprokaryotes.()第6題判斷題(1分)Ineukaryoticcells,thefirstAUGmaybeskippedifitssurroundingsequencedoesnotfittheKozak’srule.()第7題單選題(1分)Inprokaryoticcells.themRNAisrecognizedbyoftheribosome?()Asmallsubunitproteins;B5SrRNA;C16SrRNA;D23SrRNA.第8題單選題(1分)WhichofthefollowingfactorshaveGTPaseactivity()AIF1;BIF3;CeIF5B;DeIF4G.第9題單選題(1分)ThefunctionofIF2is().ABringtheinitiatortRNAtotheribosome;BBringthemRNAtotheribosome;CPreventthere-associationofthesmallandbigsubunit;DNoneofabove.第10題單選題(1分)Whichofthefollowingfactorswillbindtothefactorcenteronribosome()AIF2;BEF-Tu;CEF-G;DAllofabove.Unit14Quiz第1題判斷題(1分)ClassIreleasefactorsfunctionallymimicatRNA,havingaGGQmotifinteractswiththestopcodon.()第2題判斷題(1分)Proteinfoldingisaco-translationaleventthatoccursatthenascentpolypeptideisbeingmade.()第3題判斷題(1分)Signalsequencesareusuallycomprisedofahydrophilic,usuallypositivelychargedN-terminalregion,acentralhydrophobicdomainandaC-terminalregionwiththecleavagesiteforsignalpeptidase.()第4題單選題(1分)ThestopcodonUGAcanberecognizedbythereleasefactor().ARF1;BRF2;CeRF1;DRF2andeRF1.第5題單選題(1分)TheribosomerecyclingfactorbindstothesiteofribosomeandcooperateswithEF-GandIF3torecycleribosomesafterpolypeptiderelease.()AA;BP;CE;DAllofabove.第6題單選題(1分)The()