1、Product Data Sheet(-)-Epigallocatechin GallateCat. No.: HY-13653CAS No.: 989-51-5分式: CHO分量: 458.37作靶點(diǎn): Autophagy; Reactive Oxygen Species; HIV; Ferroptosis; MitochondrialMetabolism; Endogenous Metabolite作通路: Autophagy; Immunology/Inflammation; Metabolic Enzyme/Protease; NF-B;Anti-infection; Apoptosi

2、s儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 30 mg/mL (65.45 mM)H2O : 20 mg/mL (43.63 mM; Need ultrasonic)* means soluble, but saturation unknown.SolventMass1 mg 5 mg 10 mgConcentration制備儲(chǔ)備液1 mM 2.1816 mL 10.9082 mL 21.8164 mL5 mM 0.4363 mL 2.1816 mL 4.3633

3、 mL10 mM 0.2182 mL 1.0908 mL 2.1816 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 儲(chǔ)存時(shí)，請?jiān)?6 個(gè)內(nèi)使，-20C 儲(chǔ)存時(shí)，請?jiān)?1 個(gè)內(nèi)使。BIOLOGICAL ACTIVITY物活性 (-)-Epigallocatechin Gallate 種茶類 酮，具有強(qiáng)的抗氧化，抗炎和抗癌作。報(bào)道顯(-)-Epigallocatechin Gallate可抑制 EGFR 信號傳導(dǎo)，從發(fā)揮抗癌作1。(-)-E

4、pigallocatechin Gallate (EGCG) 氨酸脫氫酶 1/2 ( GDH1/2，GLUD1/2) 抑制劑。(-)-Epigallocatechin Gallate 通過激活細(xì)胞素c氧化酶來誘導(dǎo)氧化磷酸化。IC & Target EGFR HER2 HER3 Human EndogenousMetabolite(批量添加)Page 1 of 2 www.MedChemE體外研究 (-)-Epigallocatechin Gallate (EGCG) inhibits activation EGFR, HER2 and HER3 in the SW837 human colon

5、 cancer cellline. (-)-Epigallocatechin Gallate (10 M) also inhibits cell growth, suppresses activation of EGFR, HER2, and HER3, andcauses decrease in the levels of COX-2 and Bcl-xL proteins, and apoptosis after treatment for 96 h1. (-)-Epigallocatechin Gallate (0-35 g/mL) inhibits the proliferation

6、of colorectal cancer cells. (-)-Epigallocatechin Gallate(35 g/mL) induces apoptosis of colorectal cancer cells3. (-)-Epigallocatechin Gallate (EGCG; 50, 75 and 100 M)dose-dependently inhibits the growth of HepG2 cells, and induces apoptosis in HepG2 cells4.體內(nèi)研究 (-)-Epigallocatechin Gallate (5, 10, a

7、nd 20 mg/kg, p.o.) inhibits the growth of orthotopic colorectal cancer cells in mice3.PROTOCOLCell Assay 3 LoVo, SW480, HCT-8, and HT-29 cells are seeded in 96-well plates at a concentration of 5103 cells; each cell line istotally seeded in the 12 wells. Complete medium is added to the wells, up to

8、200 L; the medium contains 0 g/mL,10 g/mL, 20 g/mL, and 35 g/mL of (-)-Epigallocatechin Gallate. The inhibition rate=1 - (absorbance of (-)-Epigallocatechin Gallate group - absorbance of control group)/(absorbance of control group - absorbance of blankcontrol group) 1003.MCE has not independently co

9、nfirmed the accuracy of these methods. They are for reference only.Animal Mice3Administration 3 At 2 weeks postsurgery, 39 out of the 40 nude mice presented with tumors. Based on the volume of the tumors, the 39 mice with tumors are divided into four groups: a control group (n=9); a group that recei

10、ves 5 mg/kg of (-)-Epigallocatechin Gallate (n=10); a group that receives 10 mg/kg of (-)-Epigallocatechin Gallate (n=10); and a groupthat receives 20 mg/kg of (-)-Epigallocatechin Gallate (n=10). In the therapeutic groups, (-)-Epigallocatechin Gallateis administrated intragastrically, and in the co

11、ntrol group, 100 uL of physiological saline is administratedintragastrically, once daily for 14 days. After the treatment of the mice with (-)-Epigallocatechin Gallate for 4 weeks,the growth and metastasis of the primary tumors are continuously monitored using a fluorescent imaging system.After 4 we

12、eks, the primary tumors are weighed and immediately put into liquid nitrogen (196C) and 2 to 3 hourslater, these specimens are stored at 80C. In addition, the other parts of the primary tumor and metastases are fixedin 4% formaldehyde3.MCE has not independently confirmed the accuracy of these method

13、s. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Anal Chem. 2020 Feb 13. J Mol Med (Berl). 2019 Aug;97(8):1183-1193. Nanomaterials (Basel). 2019 Mar 8;9(3). pii: E396. J Cancer. 2019; 10(26): 65436556. Biochem Biophys Res Commun. 2019 Dec 2. pii: S0006-291X(19)32185-0.See more customer validations on HYPE

14、RLINK www.MedChemE www.MedChemEREFERENCES1. De Amicis F, et al. Epigallocatechin gallate inhibits growth and Epithelial-to-Mesenchymal Transition in human thyroid carcinoma cell lines. J Cell Physiol.2013 Apr 1.Page 2 of 3 www.MedChemE2. Jin H, et al. Epigallocatechin gallate inhibits the proliferat

15、ion of colorectal cancer cells by regulating Notch signaling. Onco Targets Ther. 2013;6:145-53.3. Wing Pui Tsang, et al. Epigallocatechin gallate up-regulation of miR-16 and induction of apoptosis in human cancer cells. The Journal of NutritionalBiochemistry. 2010, 21(2): 140-146.4. Peeters TH, et a

16、l. Isocitrate dehydrogenase 1-mutated cancers are sensitive to the green tea polyphenol epigallocatechin-3-gallate. Cancer Metab. 2019May 20;7:4.5. Castellano-Gonzlez G, et al. Epigallocatechin-3-gallate induces oxidative phosphorylation by activating cytochrome c oxidase in human culturedneurons and astrocytes. Oncotarget. 2016