1、Genetic transformation of wheat mediated by Agrobacterium tumefaciens作者: HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Cheng%20M&ut=A1997YF80000013&pos=1 Cheng M, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneCli

2、ckSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Fry%20JE&ut=A1997YF80000013&pos=2 Fry JE, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Pang%20SZ&ut=A1997YF80000013&pos=3 Pang SZ, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mo

3、de=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Zhou%20HP&ut=A1997YF80000013&pos=4 Zhou HP, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Hironaka%20CM&ut=A1997YF80000013&pos=5 Hironaka CM, HYPERLINK /DaisyOneClickSearch.

4、do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Duncan%20DR&ut=A1997YF80000013&pos=6 Duncan DR, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Conner%20TW&ut=A1997YF80000013&pos=7 Conner TW, HYPERLI

5、NK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Wan%20YC&ut=A1997YF80000013&pos=8 Wan YC 來源出版物: PLANT PHYSIOLOGY卷: 115期: 3頁: 971-980出版年: NOV 1997 被引頻次: HYPERLINK /CitingArticles.do?product=WOS&search_mode=CitingArticles&SID=S156KlgGfHp3A8nl7

6、Em&db_id=WOS&parentQid=41&parentDoc=1&recid=102878237 o 查看引用本文的所有文獻 230參考文獻: HYPERLINK /CitedRefList.do?product=WOS&search_mode=CitedRefList&SID=S156KlgGfHp3A8nl7Em&db_id=WOS&parentQid=41&parentDoc=1&recid=102878237 o 查看此記錄的題錄信息 36 HYPERLINK javascript:void(); o 查看引證關系圖 引證關系圖摘要: A rapid Agrobacteriu

7、m tumefaciens-mediated transformation system for wheat was developed using freshly isolated immature embryos, precultured immature embryos, and embryogenic calli as explants. The explants were inoculated with a disarmed A. tumefaciens strain C58 (ABI) harboring the binary vector pMON18365 containing

8、 the beta-glucuronidase gene with an intron, and a selectable marker, the neomycin phosphotransferase II gene. Various factors were found to influence the transfer-DNA delivery efficiency, such as explant tissue and surfactants present in the inoculation medium. The inoculated immature embryos or em

9、bryogenic calli were selected on G418-containing media. Transgenic plants were regenerated from all three types of explants. The total time required from inoculation to the establishment of plants in soil was 2.5 to 3 months. So far, more than 100 transgenic events have been produced. Almost all tra

10、nsformants were morphologically normal. Stable integration, expression, and inheritance of the transgenes were confirmed by molecular and genetic analysis. One to five copies of the transgene were integrated into the wheat genome without rearrangement. Approximately 35% of the transgenic plants rece

11、ived a single copy of the transgenes based on Southern analysis of 26 events. Transgenes in T-1 progeny segregated in a Mendelian fashion in most of the transgenic plants.文獻類型: Article 語言: English KeyWords Plus: FERTILE TRANSGENIC WHEAT; ORYZA-SATIVA-L; TRITICUM-AESTIVUM L; FOREIGN GENES; INDICA RIC

12、E; T-DNA; MICROPROJECTILE BOMBARDMENT; SELECTABLE MARKER; RAPID PRODUCTION; L PLANTS 通訊作者地址: Cheng, M (通訊作者), MONSANTO CO, 700 CHESTERFIELD PKWY N, MAIL ZONE GG4H, ST LOUIS, MO 63198 USA 出版商: AMER SOC PLANT PHYSIOLOGISTS, 15501 MONONA DRIVE, ROCKVILLE, MD 20855 學科類別: Plant Sciences IDS 號: YF800 ISSN

13、: 0032-0889 Transgene inheritance in plants genetically engineered by microprojectile bombardment作者: HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Pawlowski%20WP&ut=A1996VH13000003&pos=1 Pawlowski WP, HYPERLINK /DaisyOneClickSearch.

14、do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Somers%20DA&ut=A1996VH13000003&pos=2 Somers DA 來源出版物: MOLECULAR BIOTECHNOLOGY卷: 6期: 1頁: 17-30出版年: AUG 1996 被引頻次: HYPERLINK /CitingArticles.do?product=WOS&search_mode=CitingArticles&SID=S156KlgGfHp3A8nl7Em&db_id=WOS&pa

15、rentQid=41&parentDoc=2&recid=97447683 o 查看引用本文的所有文獻 62參考文獻: HYPERLINK /CitedRefList.do?product=WOS&search_mode=CitedRefList&SID=S156KlgGfHp3A8nl7Em&db_id=WOS&parentQid=41&parentDoc=2&recid=97447683 o 查看此記錄的題錄信息 86 HYPERLINK javascript:void(); o 查看引證關系圖 引證關系圖摘要: Microprojectile bombardment to deliver

16、 DNA into plant cells represents a major breakthrough in the development of plant transformation technologies and accordingly has resulted in transformation of numerous species considered recalcitrant to Agrobacterium- or protoplast-mediated transformation methods. This article attempts to review th

17、e current understanding of the molecular and genetic behavior of transgenes introduced by microprojectile bombardment. The characteristic features of the transgene integration pattern resulting from DNA delivery via microprojectile bombardment include integration of the full length transgene as well

18、 as rearranged copies of the introduced DNA. Copy number of both the transgene and rearranged fragments is often highly variable. Most frequently the multiple transgene copies and rearranged fragments are inherited as a single locus. However, a variable proportion of transgenic events produced by mi

19、croprojectile bombardment exhibit Mendelian ratios for monogenic and digenic segregation Vs events exhibiting segregation distortion, The potential mechanisms underlying these observations are discussed.文獻類型: Review 語言: English 作者關鍵詞: plants; stable transformation; direct DNA delivery; microprojecti

20、le bombardment; transgene expression; inheritance KeyWords Plus: DIRECT GENE-TRANSFER; AGROBACTERIUM-MEDIATED TRANSFORMATION; DISCHARGE PARTICLE-ACCELERATION; HIGH-VELOCITY MICROPROJECTILES; T-DNA; FOREIGN GENES; RICE PLANTS; PETUNIA-HYBRIDA; EXOGENOUS DNA; WHEAT PLANTS 地址: 1. UNIV MINNESOTA, DEPT A

21、GRON & PLANT GENET, ST PAUL, MN 55108 USA 出版商: HUMANA PRESS INC, 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 學科類別: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology IDS 號: VH130 ISSN: 1073-6085 Molecular characteristics of transgenic wheat and the effect on transgene express

22、ion作者: HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Stoger%20E&ut=000079800000007&pos=1 Stoger E, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Williams%20S&ut=000

23、079800000007&pos=2 Williams S, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Keen%20D&ut=000079800000007&pos=3 Keen D, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name

24、=Christou%20P&ut=000079800000007&pos=4 Christou P 來源出版物: TRANSGENIC RESEARCH卷: 7期: 6頁: 463-471出版年: NOV 1998 被引頻次: HYPERLINK /CitingArticles.do?product=WOS&search_mode=CitingArticles&SID=S156KlgGfHp3A8nl7Em&db_id=WOS&parentQid=41&parentDoc=3&recid=109731477 o 查看引用本文的所有文獻 49參考文獻: HYPERLINK /CitedRefLi

25、st.do?product=WOS&search_mode=CitedRefList&SID=S156KlgGfHp3A8nl7Em&db_id=WOS&parentQid=41&parentDoc=3&recid=109731477 o 查看此記錄的題錄信息 31 HYPERLINK javascript:void(); o 查看引證關系圖 引證關系圖摘要: A population of R-0 transgenic wheat plants, generated by particle bombardment, was analyzed to define molecular, gene

26、tic and phenotypic properties resulting from transformation with a cointegrate vector, or cotransformation with two separate plasmids. By evaluating the progeny of 70 independently-derived transgenic plants, we also identified rare events such as chimerism and transgene elimination, which provide va

27、luable information concerning the development of transgenic cereal plants following bombardment experiments. The frequency of chimerism in our transgenic wheat plants was very low Furthermore, while transgene elimination did occur, this was also a very ran event. We determined the copy numbers of in

28、tegrated transgenes and the levels of transgene expression. Comparisons to transgenic rice plants generated in the same manner demonstrated some similarities, but also important differences in transgene behavior. Whereas in rice there is no evidence for any direct relationship between transgene copy

29、 number and transgene expression or stability, multicopy populations in wheat demonstrated a bias towards higher levels of expression for the two genes and the maize ubiquitin promoter evaluated in the present study.文獻類型: Article 語言: English 作者關鍵詞: particle bombardment; transgene expression; Triticu

30、m aestivum KeyWords Plus: DISCHARGE PARTICLE-ACCELERATION; TRITICUM-AESTIVUM L; MICROPROJECTILE BOMBARDMENT; AGROBACTERIUM-TUMEFACIENS; BETA-GLUCURONIDASE; RAPID PRODUCTION; L PLANTS; T-DNA; TRANSFORMATION; MARKER 通訊作者地址: Stoger, E (通訊作者), John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk Engla

31、nd 地址: 1. John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk England 出版商: KLUWER ACADEMIC PUBL, SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS 學科類別: Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology IDS 號: 187RF ISSN: 0962-8819 Agro

32、bacterium-mediated large-scale transformation of wheat (Triticum aestivum L.) using glyphosate selection作者: HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Hu%20T&ut=000183826400013&pos=1 Hu T, HYPERLINK /DaisyOneClickSearch.do?produc

33、t=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Metz%20S&ut=000183826400013&pos=2 Metz S, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Chay%20C&ut=000183826400013&pos=3 Chay C, HYPERLINK /DaisyOneClickSear

34、ch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Zhou%20HP&ut=000183826400013&pos=4 Zhou HP, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Biest%20N&ut=000183826400013&pos=5 Biest N, HYPERLINK /D

35、aisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Chen%20G&ut=000183826400013&pos=6 Chen G, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Cheng%20M&ut=000183826400013&pos=7 Cheng M

36、, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Feng%20X&ut=000183826400013&pos=8 Feng X, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Radionenko%20M&ut=0001838264

37、00013&pos=9 Radionenko M, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Lu%20F&ut=000183826400013&pos=10 Lu F, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Fry%20J

38、&ut=000183826400013&pos=11 Fry J 來源出版物: PLANT CELL REPORTS卷: 21期: 10頁: 1010-1019出版年: JUN 2003 被引頻次: HYPERLINK /CitingArticles.do?product=WOS&search_mode=CitingArticles&SID=S156KlgGfHp3A8nl7Em&db_id=WOS&parentQid=41&parentDoc=4&recid=130294758 o 查看引用本文的所有文獻 41參考文獻: HYPERLINK /CitedRefList.do?product=

39、WOS&search_mode=CitedRefList&SID=S156KlgGfHp3A8nl7Em&db_id=WOS&parentQid=41&parentDoc=4&recid=130294758 o 查看此記錄的題錄信息 48 HYPERLINK javascript:void(); o 查看引證關系圖 引證關系圖摘要: An Agrobacterium-mediated transformation system with glyphosate selection has been developed for the large-scale production of trans

40、genic plants. The system uses 4-day precultured immature embryos as explants. A total of 30 vectors containing the 5-enol-pyruvylshikimate-3-phosphate synthase gene from Agrobacterium strain CP4 (aroA:CP4), which confers resistance to glyphosate, were introduced into wheat using this system. The aro

41、A:CP4 gene served two roles in this study-selectable marker and gene of interest. More than 3,000 transgenic events were produced with an average transformation efficiency of 4.4%. The entire process from isolation of immature embryos to production of transgenic plantlets was 50-80 days. Transgenic

42、events were evaluated over several generations based on genetic, agronomic and molecular criteria. Forty-six percent of the transgenic events fit a 3:1 segregation ratio. Molecular analysis confirmed that four of six lead transgenic events selected from Agrobacterium transformation contained a singl

43、e insert and a single copy of the transgene. Stable expression of the aroA:CP4 gene was confirmed by ELISA through nine generations. A comparison of Agrobacterium-mediated transformation to a particle bombardment system demonstrated that the Agrobacterium system is reproducible, has a higher transfo

44、rmation efficiency with glyphosate selection and produces higher quality transgenic events in wheat. One of the lead events from this study, no. 33391, has been identified as a Roundup Ready wheat commercial candidate.文獻類型: Article 語言: English 作者關鍵詞: Agrobacterium transformation; wheat (Triticum aes

45、tivum L.); glyphosate selection; Roundup Ready KeyWords Plus: FERTILE TRANSGENIC WHEAT; MICROPROJECTILE BOMBARDMENT; RICE PLANTS; GENETIC-TRANSFORMATION; BETA-GLUCURONIDASE; RAPID PRODUCTION; TUMEFACIENS; PROMOTER; EXPRESSION; INDICA 通訊作者地址: Hu, T (通訊作者), Monsanto Co, 700 Chesterfield Pkwy W, St Lou

46、is, MO 63017 USA 地址: 1. Monsanto Co, St Louis, MO 63017 USA 出版商: SPRINGER-VERLAG, 175 FIFTH AVE, NEW YORK, NY 10010 USA 學科類別: Plant Sciences IDS 號: 695JB ISSN: 0721-7714 DOI: 10.1007/s00299-003-0617-6 Transgenic barley expressing a fungal xylanase gene in the endosperm of the developing grains作者: HY

47、PERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Patel%20M&ut=000086760700013&pos=1 Patel M, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Johnson%20JS&ut=00008676070001

48、3&pos=2 Johnson JS, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Brettell%20RIS&ut=000086760700013&pos=3 Brettell RIS, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&nam

49、e=Jacobsen%20J&ut=000086760700013&pos=4 Jacobsen J, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Xue%20GP&ut=000086760700013&pos=5 Xue GP 來源出版物: MOLECULAR BREEDING卷: 6期: 1頁: 113-123出版年: FEB 2000 被引頻次: HYPERLINK /CitingArticles.do?p

50、roduct=WOS&search_mode=CitingArticles&SID=S156KlgGfHp3A8nl7Em&db_id=WOS&parentQid=41&parentDoc=5&recid=114835483 o 查看引用本文的所有文獻 41參考文獻: HYPERLINK /CitedRefList.do?product=WOS&search_mode=CitedRefList&SID=S156KlgGfHp3A8nl7Em&db_id=WOS&parentQid=41&parentDoc=5&recid=114835483 o 查看此記錄的題錄信息 38 HYPERLINK

51、javascript:void(); o 查看引證關系圖 引證關系圖摘要: The feasibility of producing plant cell wall polysaccharide-hydrolysing feed enzymes in the endosperm of barley grain was investigated. The coding region of a modified xylanase gene (xynA) from the rumen fungus, Neocallimastix patriciarum, linked with an endospe

52、rm-specific promoter from cereal storage protein genes was introduced into barley by Agrobacterium-mediated transformation. Twenty-four independently transformed barley lines with the xylanase gene were produced and analysed. The fungal xylanase was produced in the developing endosperm under the con

53、trol of either the rice glutelin B-1 (GluB-1) or barley B1 hordein (Hor2-4) promoter. The rice GluB-1 promoter provided an apparently higher expression level of recombinant proteins in barley grain than the barley Hor2-4 promoter in both transient and stable expression experiments. In particular, th

54、e mean value for the fungal xylanase activity driven by the GluB-1 promoter in the mature grains of transgenic barley was more than twice that with the Hor2-4 promoter. Expression of the xylanase transgene under these endosperm-specific promoters was not observed in the leaf, stem and root tissues.

55、Accumulation of the fungal xylanase in the developing grains of transgenic barley followed the pattern of storage protein deposition. The xylanase was stably maintained in the grain during grain maturation and desiccation and post-harvest storage. These results indicate that the cereal grain express

56、ion system may provide an economic means for large scale production of feed enzymes in the future.文獻類型: Article 語言: English 作者關鍵詞: endosperm; expression; feed enzyme; seed storage protein; transgenic barley; xylanase KeyWords Plus: NEOCALLIMASTIX-PATRICIARUM; ESCHERICHIA-COLI; PARTICLE BOMBARDMENT;

57、TOBACCO PLANTS; HORDEIN GENE; WHEAT; TRANSFORMATION; SEQUENCE; AGROBACTERIUM; (TRANS)GENES 通訊作者地址: Xue, GP (通訊作者), CSIRO, 306 Carmody Rd, St Lucia, Qld 4067 Australia 地址: 1. CSIRO, St Lucia, Qld 4067 Australia2. CSIRO, Canberra, ACT 2601 Australia 出版商: KLUWER ACADEMIC PUBL, SPUIBOULEVARD 50, PO BOX

58、17, 3300 AA DORDRECHT, NETHERLANDS 學科類別: Agronomy; Plant Sciences; Genetics & Heredity; Horticulture IDS 號: 309GT ISSN: 1380-3743 A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques作者: HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mod

59、e=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Travella%20S&ut=000227561200002&pos=1 Travella S, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Ross%20SM&ut=000227561200002&pos=2 Ross SM, HYPERLINK /DaisyOneClickSearch.do?

60、product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Harden%20J&ut=000227561200002&pos=3 Harden J, HYPERLINK /DaisyOneClickSearch.do?product=WOS&search_mode=DaisyOneClickSearch&db_id=&SID=S156KlgGfHp3A8nl7Em&name=Everett%20C&ut=000227561200002&pos=4 Everett C, HYPERLINK /D