流式細(xì)胞術(shù)在白血病、淋巴瘤免疫分型中的應(yīng)用流式細(xì)胞術(shù)1免疫分型的作用區(qū)分淋系、髓系白血病■區(qū)分淋系白血病中的T、B-ALL■鑒定混合細(xì)胞性白血病的類型于治療過程中監(jiān)測MRD,評價(jià)療效■提示某些遺傳學(xué)異常CD56+API-t(11;17)?CD19+AM+(8:21)(q22;q21)提示預(yù)后CL7、CD11b、CD56等陽性的AML患者預(yù)后不良CD15陽性的AML患者CR率高免疫分型的作用2免疫分型的原理■正常造血細(xì)胞白血病細(xì)胞抗原表達(dá)遵循系列保守性出現(xiàn)抗原跨系表達(dá)髓系CD33+,T系CD髓系:CD33+CD7+抗原表達(dá)有一定的時(shí)序性出現(xiàn)抗原時(shí)序混亂表達(dá)原始細(xì)胞CD34+CD11I原始細(xì)胞CD34+CD11B+各階段細(xì)胞按一定的比例分布出現(xiàn)分化阻滯原幼細(xì)胞<5%原幼細(xì)胞>20免疫分型的原理3免疫分型需要掌握什么理論知識?■正常造血細(xì)胞的抗原表達(dá)規(guī)律不同系列的細(xì)胞表達(dá)的抗原?■同一系列不同階段的細(xì)胞表達(dá)的抗原?每種抗原的表達(dá)強(qiáng)度免疫分型需要掌握什么理論知識?4正常B系細(xì)胞分化圖CDIoCD45rFMC7CDIgCD20CD221CDSB淋巴細(xì)胞成熟過程源丁王建中等編著的《臨床流式細(xì)胞分析》正常B系細(xì)胞分化圖5流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件6流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件7流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件8流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件9流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件10流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件11流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件12流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件13流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件14流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件15流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件16流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件17流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件18流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件19流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件20流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件21流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件22流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件23流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件24流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件25流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件26流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件27流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件28流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件29流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件30流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件31流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件32流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件33流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件34流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件35流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件36流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件37流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件38流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件39流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件40流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件41流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件42流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件43流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件44流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件45流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件46流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件47流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件48流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件49流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件50流式細(xì)胞術(shù)在白血病、淋巴瘤免疫分型中的應(yīng)用流式細(xì)胞術(shù)51免疫分型的作用區(qū)分淋系、髓系白血病■區(qū)分淋系白血病中的T、B-ALL■鑒定混合細(xì)胞性白血病的類型于治療過程中監(jiān)測MRD,評價(jià)療效■提示某些遺傳學(xué)異常CD56+API-t(11;17)?CD19+AM+(8:21)(q22;q21)提示預(yù)后CL7、CD11b、CD56等陽性的AML患者預(yù)后不良CD15陽性的AML患者CR率高免疫分型的作用52免疫分型的原理■正常造血細(xì)胞白血病細(xì)胞抗原表達(dá)遵循系列保守性出現(xiàn)抗原跨系表達(dá)髓系CD33+,T系CD髓系:CD33+CD7+抗原表達(dá)有一定的時(shí)序性出現(xiàn)抗原時(shí)序混亂表達(dá)原始細(xì)胞CD34+CD11I原始細(xì)胞CD34+CD11B+各階段細(xì)胞按一定的比例分布出現(xiàn)分化阻滯原幼細(xì)胞<5%原幼細(xì)胞>20免疫分型的原理53免疫分型需要掌握什么理論知識?■正常造血細(xì)胞的抗原表達(dá)規(guī)律不同系列的細(xì)胞表達(dá)的抗原?■同一系列不同階段的細(xì)胞表達(dá)的抗原?每種抗原的表達(dá)強(qiáng)度免疫分型需要掌握什么理論知識?54正常B系細(xì)胞分化圖CDIoCD45rFMC7CDIgCD20CD221CDSB淋巴細(xì)胞成熟過程源丁王建中等編著的《臨床流式細(xì)胞分析》正常B系細(xì)胞分化圖55流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件56流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件57流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件58流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件59流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件60流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件61流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件62流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件63流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件64流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件65流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件66流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件67流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件68流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件69流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件70流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件71流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件72流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件73流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件74流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件75流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件76流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件77流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件78流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件79流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件80流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件81流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件82流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件83流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件84流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件85流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件86流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件87流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件88流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件89流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件90流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件91流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件92流式細(xì)胞術(shù)在白血病和淋巴瘤中應(yīng)用課件93