Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEHDAC8-IN-1Cat.No.:HY-111342CASNo.:1417997-93-3分?式:C??H??NO?分?量:345.39作?靶點(diǎn):HDAC作?通路:CellCycle/DNADamage;Epigenetics儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:50mg/mL(144.76mM;ultrasonicandwarmingandheatto60°C)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.8953mL14.4764mL28.9528mL5mM0.5791mL2.8953mL5.7906mL10mM0.2895mL1.4476mL2.8953mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(7.24mM);Clearsolution1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(7.24mM);Clearsolution3.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(7.24mM);ClearsolutionBIOLOGICALACTIVITY?物活性HDAC8-IN-1?個(gè)HDAC8抑制劑，其IC50值為27.2nM。IC50&TargetHDAC827.2nM(IC50)體外研究HDAC8-IN-1isaHDAC8inhibitorwithanIC50of27.2nMincancercelllines.HDAC8-IN-1(compound22d)showsantiproliferativeeffectstowardseveralhumanlungcancercelllines(A549,H1299,andCL1-5);HDAC8-IN-1exhibitscytotoxicityagainsthumanlungCL1-5cellswithoutsignificantcytotoxicityfornormalIMR-90cells[1].戶(hù)使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?CellDeathDis.2021May18;12(6):501.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].HuangWJ,etal.Synthesisandbiologicalevaluationofortho-arylN-hydroxycinnamidesaspotenthistonedeacetylase(HDAC)8isoform-selectiveinhibitors.ChemMedChem.2012Oct;7(10):1815-24.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400