Chapter24Immunediversity24.1Introduction

24.2Clonalselectionamplifieslymphocytesthatrespondtoindividualantigens

24.3Immunoglobulingenesareassembledfromtheirpartsinlymphocytes

24.4Lightchainsareassembledbyasinglerecombination

24.5Heavychainsareassembledbytworecombinations

24.6Recombinationgeneratesextensivediversity

24.7Avianimmunoglobulinsareassembledfrompseudogenes

24.8Immunerecombinationusestwotypesofconsensussequence

24.9Recombinationgeneratesdeletionsorinversions

24.10TheRAGproteinscatalyzebreakageandreunion

24.11Allelicexclusionistriggeredbyproductiverearrangement

24.12DNArecombinationcausesclassswitching

24.13EarlyheavychainexpressioncanbechangedbyRNAprocessing

24.14Somaticmutationgeneratesadditionaldiversity

24.15Bcelldevelopmentandmemory

24.16T-cellreceptorsarerelatedtoimmunoglobulins

24.17ThemajorhistocompatibilitylocuscodesformanygenesoftheimmunesystemAntigenisanymoleculewhoseentryintoanorganismprovokessynthesisofanantibody(immunoglobulin).

Superfamilyisasetofgenesallrelatedbypresumeddescentfromacommonancestor,butnowshowingconsiderablevariation.

TcellsarelymphocytesoftheT(thymic)lineage;maybesubdividedintoseveralfunctionaltypes.TheycarryTcR(T-cellreceptor)andareinvolvedinthecell-mediatedimmuneresponse.24.1IntroductionFigure24.1Humoralimmunityisconferredbythebindingoffreeantibodiestoantigenstoformantigen-antibodycomplexesthatareremovedfromthebloodstreambymacrophagesorthatareattackeddirectlybythecomplementproteins.24.1IntroductionFigure24.2Incell-mediatedimmunity,killerTcellsusetheT-cellreceptortorecognizeafragmentoftheforeignantigenwhichispresentedonthesurfaceofthetargetcellbytheMHCprotein.24.1IntroductionHaptenisasmallmoleculethatactsasanantigenwhenconjugatedtoaprotein.24.2Clonalselectionamplifieslymphocytesthatrespondtoindividualantigens

Figure24.3ThepoolofimmaturelymphocytescontainsBcellsandTcellsmakingantibodiesandreceptorswithavarietyofspecificities.Reactionwithanantigenleadstoclonalexpansionofthelymphocytewiththeantibody(Bcell)orreceptor(Tcell)thatcanrecognizetheantigen.24.2ClonalselectionamplifieslymphocytesthatrespondtoindividualantigensCgenescodefortheconstantregionsofimmunoglobulinproteinchains.

Vgeneissequencecodingforthemajorpartofthevariable(N-terminal)regionofanimmunoglobulinchain.24.3Immunoglobulingenesareassembledfromtheirpartsinlymphocytes

Figure24.4Heavyandlightchainscombinetogenerateanimmunoglobulinwithseveraldiscretedomains.24.3ImmunoglobulingenesareassembledfromtheirpartsinlymphocytesFigure24.4Heavyandlightchainscombinetogenerateanimmunoglobulinwithseveraldiscretedomains.24.3ImmunoglobulingenesareassembledfromtheirpartsinlymphocytesTable24.1EachimmunoglobulinfamilyconsistsofaclusterofVgeneslinkedtoitsCgene(s).24.3Immunoglobulingenesareassembledfromtheirpartsinlymphocytes

FamilyVGenesCGenesManMouseManMouseLambda<3002>64Kappa<300~100011Heavy~300>100098Figure24.5ThelambdaCgenesegmentisprecededbyaJsegment,sothatV-Jrecombinationgeneratesafunctionallambdalight-chaingene.24.3ImmunoglobulingenesareassembledfromtheirpartsinlymphocytesFigure24.6ThekappaCgenesegmentisprecededbymultipleJsegmentsinthegermline.V-JjoiningmayrecognizeanyoneoftheJsegments,whichisthensplicedtotheCgenesegmentduringRNAprocessing.24.3ImmunoglobulingenesareassembledfromtheirpartsinlymphocytesFigure24.7Heavygenesareassembledbysequentialjoiningreactions.FirstaDsegmentisjoinedtoaJsegment;thenaVgenesegmentisjoinedtotheDsegment.24.3ImmunoglobulingenesareassembledfromtheirpartsinlymphocytesFigure24.8ThelambdafamilyconsistsofVgenesegmentslinkedtoasmallnumberofJ-Cgenesegments.24.4Thediversityofgermlineinformation

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