productivityproductivitytoSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZHPLC-UV-VisabsorptionandchargedaerosoldetectionDietarysupplementsandbotanicalnaturalproductsapplicationsnotebookComplexsamples,powerfulchromatographicanalysisGlossarySummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesThermoFisherSClENTlFlcSummaryOverviewSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossarysupplementsandbotanicalnaturalproductspresentsspecificchallengeswithrespectto:samplematricescanaffecttheefficiencyandreproducibilityofextraction.2.Separation:anexceedinglywiderangeofchemicalstructureswillrequiredifferentchromatographicapproaches.3.Detection:analyteslackingachromophorearenotdetectedbyabsorptiondetectors.standardsavailable.ReliableanalysisofdietarysupplementsThisnotebookoffersReliableanalysisofdietarysupplementsandbotanicalnaturalproductsisandbotanicalnaturalproductsisvitalinensuringtheconsistentqualityandsafetyofrawmaterialsandendproducts.However,analysisparticularlydemanding.Botanicalnaturalproducts,forexample,areoftencomplexmixturcompositionsmaydifferundertheinfluenceofvariablessuchasgenetics,cultivationconditions,adulteration,storageconditions,andprocessingmethods.Chromatographicanalysisofbothdietaryperformanceliquidchromatography(HPLC)combinedwithchargedaerosoldetection,absorptiondetectionorbothcanbeusedtomeettheneedsofscientistsworkingwithdietarysupplementsandbotanicalnaturalproducts.WhatisWhatismeantbydietarysupplementsandbotanicalnaturalproducts?SummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossarySummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossarySummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryOutercircle:DietarysupplementmarketdistributionbyregionsworthInnercircle:DietarysupplementmarketdistributionbyingredienEstimatedgrowthofplant-derivedandbotanicaldrugsmarkets.Compoundannualgrowthrate(CAGR)shownforbotSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryRelativeRepsonseFactorRelativeRepsonseFactor2.521.510.50AcetaminophenChloramphenicolCortisoneD-GlucoseDibucaineDiclofenacDiuronD-PantothenicacidFluorescineGuanineHippuricacidLactoseL-AscorbicAcidMaltoseMe?oquineNewcoccineRhodamine800SaccharinSucroseTripolidineUracilUridineSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryColumnCompartmentColumnCompartmentChargedAerosolDetectorOpticalDetector0eluterelutereluterelutereluterSplitSamplertheleftpumpensuresthatenteringthedetectorremainsduringagradient(ofthegradient(grSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryCollectivepowerofchromatographyInstrumentsconfiguredtomeetCollectivepowerofchromatographyInstrumentsconfiguredtomeetyourexactmeasurementneeds1MixingChamberCoronaCharger32SummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossarySummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryAutomatesamplepreparationwithan neutralionicChoosingbetweengeneralpurneutralionicChoosingbetweengeneralpurHydrophilicinteraction:AccucoreHILIC,HypersilGOLDHILICAcclaimHILIC-10Hydrophobicinteraction:C1<C4<C8<C18/C30Aromaticcompounds:AccucoreBiphenyl,HypersilGOLDPFPBasiccompounds:AcclaimPolarAdvantage2,AccucorePolarPremiumReversed-PhaseColumnsMixturesofcharged,hydrophobic,andhydrophiliccompounds:AcclaimTrinityP1&P2HILICMixedModeIoniccompounds:Hypercarb,HypersilAX,SAXIEXpolarnonpolarSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossary??????????SummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryThermoScientificTMVanquishTMCoreHPLCsystemThermoScientificTMVanquishTMFlexUHPLCsystemThermoScientificTMVanquishTMHorizonUHPLCsystemThermoScientificTMVanquishTMDuoHPLCandUHPLCsystemsSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryCost-effectiveCost-effectivereliableUnmatchedall-roundperformanceofCADdetectionarraydetectionMeasurementandanalysisMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryState-of-the-artinstrumentationisessentialforsuccessfulsampleanalysisusingScientificChargedAerosolDetectors(CAD),advancedcolumnSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketandThermoScientific?Corona?Veo?ChargedAerosolthermoscientfcthermoscientfcSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryVariablewavelengthdetectorsoffersensitive,accurateUV-VisdetectionDetectionDetectionDiodearraydetectorscombineflexibdetectionCapsulesortabletsOpuntialeptocaulis

2915

HG5

—

3165

3126AuthenticHoodiagordoniiabilitytodifferentiatebetweenauthenticdriedplant,capsulesandtablets,andanadulterantOpuntialeptocaulissamples.SeePosterNote70540forgreaterdetail.SummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryAcetylcarnitineandlipoicacidinasupplementArtemisininAshwagandhaBacopaBlackcohoshBoswelliaCalciumandmagnesiumCarallumaCaulisloniceraeCholineCyclotidesEchinaceaFalcarinol,falcarindiolandfalcarindiol-3-acetateFlavonoids-anthocyaninsFlavonoids-anthocyaninsFlavonoids-procyanidinsGiantknotweedrhizomeGingkoFlavonoids-FloschrysanthemaFlavonoids-hesperidinGinsengHopsGotuKolaAcetylcarnitineandlipoicacidinasupplementArtemisininAshwagandhaBacopaBlackcohoshBoswelliaCalciumandmagnesiumCarallumaCaulisloniceraeCholineCyclotidesEchinaceaFalcarinol,falcarindiolandfalcarindiol-3-acetateFlavonoids-anthocyaninsFlavonoids-anthocyaninsFlavonoids-procyanidinsGiantknotweedrhizomeGingkoFlavonoids-FloschrysanthemaFlavonoids-hesperidinGinsengHopsGotuKolaMangosteenMilkthistleMogrosidePhenols–globalmethodPhytoestrogens–isoflavonesand theiraglyconesSchisandra-schizandrin,schizandrinA,andschizandrinBPunicalaginsPyrethrins–ChrysanthemumcinerariifoliumSemenraphaniSteviaStJohn’sWortPhytoestrogens–coumestansandmammalianlignansUrsolicacidandothertriterpenoidsVitaminD3insupplementsZanthoxylumnitidumTurmeric-curcuminoidsSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryAristolochiaAristolochiaCarnitineCarnitineFlavonoids-Flavonoids-catechinsHoodiaHoodiaPhytosterolsPhytosterolsVitamins–simultaneousVitamins–simultaneousdeterminationofwaterandfatsolubletomatidineSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryOOHSS21HPLC-UVanalysisofCH3CH3OCH3OOH OH HPLC-CADanalysisofamethanolicextractofAristolochiafangchiOOOOSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryCH3CH3SubstancesA-CHCH3HOHHOOHOCH3CH2CH3OOOSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryComparisonbetweenCADandUVabsorbancedetectionforHPLCseparationofashwagandhaextract.TheshadedareashowsthatmanycomponentsaremissedbytheHPLC-UVapprSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryComparisonofHPLCwitheitherCADorUVdetectiomonnieriextract.NoteUVdetectionmissesearlyelutingaCH3CH3HHOCH3OCH3CH3HOHOOOCH3OOHOHOOOHSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryOHOH35.26-Deoxycimifuoside36.Actein37.27-Deoxyactein38.Acetylshengmanolxyloside45.CimiracemosideCandE52.CimicifugosideHPLC-CADanalysisofblackcohosheOCH3OHOOOHOOCH3OActeinCH3OHSummarySummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossarySummaryOverview:DietarysupplementsSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryβ462351DeterminationofboswellicacidsinHCH3HCH3CH3CH3CH3 OH Oα-Boswellicacidα-BoswellicacidOH1212Determinationofboswellicacidsin2.3-acetyl-11-keto-αthermoscientfcthermoscientfc4520SubstancesA-CSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryAnalysisofoxypregnaneglycnotdetectedbyHPLC-UVdetectionandthatthisapproachsuffersfrombaselineperturbationthroughouOOCH3OOCarallumafimbriataSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossary21SubstancesA-CHPLC-UVanalysisofOthermoscientfcthermoscientfcSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossarySummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossarySummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossary4000641325BA4000641325BA4.CaffeicacidOOOOHHCOHHHCOHHOOHHOOOOHOOOHHOOOOOOOHOOHOHHOOHOHOHOOHOOOHHCOOOHHOOHOHSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossarythermoscientfcthermoscientfcHPLC-CADmeasurementofsodium,c2mg/mLcholinecitratesample.ThesampleimSpectrometer.OOHOH +NOCH3bitartrate NOO-OOOcitrateCycloviolacinO2ABCycloviolacinO2AB30.025.020.02.04.06.08.010.012.014.0SummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossarySummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryOHOHOOHOHOHOHOHSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryOHOHOOHOHOHOHOH14DeterminationofphenoliccompoundsOHOOOOCOHCOHOOHOOOOOHOHOOOOOHOOOOOOOOHOOOOOHOOOOOOOOOHOHOHCichoricacidChlorogenicacidEchinacosideSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossarySeparationofafifteenanthocyaninstandaSubstancesD-GHHHHHHR3’HR4’R5’HHR3'R4'R7R5'R3R6R5SummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryResponse(mAU)92351486720SummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsResponse(mAU)92351486720SummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesH-MSubstancesN-TSubstancesU-Z5007.004506.004005.003503004.002503.002002.001.00SubstancesD-G500.000-1.00-50447386950.01.02.03.04.05.06.07.08.09.010.011.012.013.014.015.00.001.002.003.004.000.01.02.03.04.05.06.07.08.09.010.011.012.013.014.015.0porousAccucoreC18column.SeeAN1042fflowrate,temperatureandgradientAN281.GlossaryAN281.AN281.SummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossarySummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesD-GSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryStructuresofcatechinsfr AnalysisofcatechiChromatogramofaFloschrysanthSubstancesD-GSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryLuteolin-7-O-glucosideLuteolin-7-O-glucosideDC BADC BASubstancesD-GCH3OOOOOOSummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesH-MSubstancesN-TSubstancesU-ZGlossarySummaryOverview:DietarysupplementsandbotanicalnaturalproductsOverview:GlobalmarketMeasurementandanalysisInstrumentationSamplepreparationSeparationDetectionAuthenticationofsupplementsApplicationexamplesSubstancesA-CSubstancesH-MSubstancesN-TSubstancesU-ZGlossaryOHOHOHCATD1-B2TRI-D1TRI-C1CATD1-B2TRI-D1TRI-C1TET-DPENTTRI-D1TRI-LTTRI-ABTRI-D1TRI-LTTRI-ABTET-PTET-PCATD1-B2TET-CTRI-D1TRI-D1TRI-B1TRITET-PTET-PCATD1-B2TET-CTRI-D1TRI-D1TRI-B1TRI-B1TRI-LTTRI-LTTRI-ABTET-DTRI-ABPENTD1-A2A-typeD1-B1CATD1-B2TRI-C1TET-DD1-B1CATD1-B2TRI-C1TET-DPENTD1-A2HPLC-CADanalysisofA-type