title細(xì)胞生物學(xué)(廈門大學(xué))中國(guó)大學(xué)mooc答案100分最新版contentIntroductionofMolecularcellbiologyCelltheory-test1、WhatdidLeeuwenhoekdo?答案:Usedsimplelightmicroscopeandsawmicroorganisms2、Hediscoveredthatallanimalsweremadeofcells,whichcontributedtothedevelopmentofthecelltheory:答案:TheodorSchwann3、Whowasthefirstpersontoseecellsunderthemicroscopeandgivethemaname?答案:RobertHooke4、WhichofthefollowingisNOToneofthemaincomponentsofthecelltheory?答案:cellsmustcontainDNACulturingandvisualizingcellsCulturingandvisualizingcells-test1、

Themostdirectsourceofmonoclonalantibodiesare

__cells.答案:Hybridoma2、Thefluorescentpropertiesofdyessuchas

FITCcanprovideinformationonthe答案:locationofspecificproteins.3、Whatfactorsnecessaryforgrowthofanimalcellsincultureareprovidedbyserum?答案:growthfactors

4、WhatistheadvantageofusingGFP-tagtovisualizespecificproteins,insteadofstainingcellswithfluorescentdyesthatbindtothetargetprotein?答案:GFPfusionsenableonetotrackchangesinthelocationoftheproteininlivingcells;stainingusuallyrequiresfixedcells5、Whichonecleavespre-miRNAinthecytoplasmtoproducemiRNA?答案:Dicer6、Asinglemonoclonalantibodycanbindtoseveraldifferentsitesonanantigen答案:錯(cuò)誤7、RNAicanbemediatedjustbyintroducingthealreadyprocessedmiRNA/siRNAintocells答案:正確8、_______

degradesmRNAswithsequencescomplementarytoaboundsiRNA

答案:RISCBiomembraneStructureBiomembraneStructure-test1、Allthefollowingstatementsdescribebiomembranesexcept:答案:

Somebiomembraneshavefreeedges.2、

Themajorsiteoflipidsynthesisineukaryoticcellsisthe答案:Endoplasmicreticulum3、Phospholipidswithshortorunsaturatedfattyacylchains答案:increasemembranefluidity.4、

The

_

aretransmembraneproteins.答案:integralmembraneproteins5、Glycolipidsandglycoproteinsareespeciallyabundantinthe答案:plasmamembrane6、Phosphatecontaininglipidsincludeallofthefollowingexcept

答案:triglyceride7、Alipidbilayeristhefundamentalstructuralcomponentofallcellmembranes.答案:正確8、MaintenanceofthelipidbilayerintheplasmamembranerequiresspecialenzymesandthehydrolysisofATP.答案:錯(cuò)誤9、Themostabundantlipidsincellmembranesare

____.

答案:phospholipids10、Sugar-containinglipidscalled

______arefoundonlyintheouterhalfofthebilayerandtheirsugargroupsareexposedacthecellsurface.

答案:glycolipidsTransmembranetransportofIonsandsmallmolecules1、Classesofmembranetransportproteinsincludeallofthefollowingexcept答案:proteintranslocons.2、TheglucoseuniporterGLUT1hasaKmof1.5mMforD-glucoseand30mMforD-galactose.

Ataconcentrationof5mMforeach,whatistherateofglucosetransportrelativetogalactosetransport?

TheVmaxmaybeassumedtobethesameforboth.答案:5.5-foldfaster3、Howdoesuniporttransportcomparetosimplediffusion?答案:Uniporttransportismuchfasterandmorespecificthansimplediffusion.4、WhichofthefourclassesofATP-poweredpumpsshareoverallsimilarity:severalsubunits,thesamegeneralorganization,andasimilarfunctioninbeingH+transporters?答案:F-classpumpsandV-classpumps5、HowdoesinhibitionoftheNa+/K+ATPaseincreasetheforceofheartmusclecontraction?答案:ItincreasescytosolicNa+andthereforedecreasesCa2+export6、Channelproteins(suchasthevoltage-gatedNachannel)transportionsmuchfasterthancarrierproteins(suchastheNa-Kpump),buttheycannotbecoupledtoanenergysource;therefore,transportmediatedbychannelsisalwayspassive.答案:正確7、TherestingmembranepotentialofatypicalanimalcellarisespredominantlythroughtheactionoftheNa-Kpump,whichineachcycletransfers3Naionsoutofthecelland2Kintothecell,leavinganexcessofnegativechargesinsidethecell.答案:錯(cuò)誤8、

Theconcentrationgradientforachargedsoluteandthemembranepotentialconstitutethe

_______gradientforthatsolute.

答案:electrochemical9、Overexpressionofthe

____inhumancancercellscanmakethesecellssimultaneouslyresistanttoavarietyofchemicallyunrelatedcytotoxicdrugsthatwewidelyusedincancerchemotherapy.

答案:Multidrug-resistancetransportprotein10、IntheepithelialcellsofthegutNa+-linkedsymportersintheapical(absorptive)domainoftheplasmamembraneandNa+-independenttransportproteinsinthebasolateraldomainbringaboutthe

_______ofabsorbedsolutes.

答案:TranscellulartransportCellularEnergetics1、Wheredoesglycolysistakesplace?答案:Cytosol2、Whereistheelectrontransportchainfoundinplantcells?答案:Thylakoidmembranesofchloroplasts3、EnergyreleasedbytheelectrontransportchainisusedtopumpH+

ionsintowhichlocation?答案:Mitochondrialintermembranespace4、Insideanactivemitochondrion,mostelectronsfollowwhichpathway?答案:Citricacidcycle–>NADH–>electrontransportchain–>oxygen5、Whichdothecatabolicproductsoffattyacidbreakdownenterintothecitricacidcycle?答案:AcetylCoA6、Brown-fatmitochondriauncoupleoxidativephosphorylationtoproduce____答案:Heat7、DuringATPsynthesis,protonsmove“down”theirelectrochemicalgradientthrough

theF0complexofATPsynthase答案:正確8、Theoutermitochondrialmembraneisderivedfromthebacterialplasmamembrane答案:錯(cuò)誤9、____isalipidsolublemoleculethatactstoshuttleelectronswithinthemitochondrialinnermembrane

答案:CoQ

分析：CoenzymeQ10、Theenergyreleasedbythepassageofelectronsalongtherespiratorychainisstoredasan_____acrosstheinnermitochondrialmembrane.

答案:protonmotiveforce

分析：electrochemicalgradientMovingProteinsintoMembranesandOrganelles1、TypeImembraneproteinshaveallofthefollowingpropertiesexcept答案:internalsignal-anchorsequence2、

Manyperoxisomalmatrixproteinsareimportedas答案:foldedproteins3、GPI-anchoringservesaspecialfunction,especiallyinpolarizedepithelialcellsbecausethismodificationservestotargetproteinstothe答案:Plasmamembrane4、Glycosylation,apost-translationalmodificationtoproteins,occursinthe答案:ER5、

Proteinsequencesfortargetingtomitochondriaislocatedat答案:theN-terminusoftheprecursorprotein.6、Mitochondrialsignalpeptidesareamphipathicαhelicalstructureswithpositivelychargedaminoacidonesideandnegativelychargedaminoontheotherside.答案:錯(cuò)誤7、Inmamm