1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEJNK-IN-7Cat. No.: HY-15617CAS No.: 1408064-71-0Synonyms: JNK inhibitor分式: CHNO分量: 493.56作靶點(diǎn): JNK作通路: MAPK/ERK Pathway儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 33.33 mg/mL (67.53 mM; N

2、eed ultrasonic)H2O : 40% PEG300 5% Tween-80 45% salineSolubility: 2.75 mg/mL (5.57 mM); Clear solution2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.75 mg/mL (5.57 mM); Suspended solution; Need ultrasonic1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEBIOLOGICAL ACTIVITY物活性 JNK-IN-7

3、種有效的 JNK 抑制劑，抑制 JNK1，JNK2 和 JNK3，IC50 分別為 1.5，2 和 0.7 nM。IC50 & Target JNK3 JNK1 JNK20.7 nM (IC50) 1.5 nM (IC50) 2 nM (IC50)體外研究 JNK-IN-7 is a relatively selective JNK inhibitor in cells. In addition to JNK 1, 2, 3, JNK-IN-7 also binds toIRAK1(IC50=14.1 nM), YSK4 (IC50=4.8 nM), ERK3 (IC50=22 nM), PI

4、K3C3, PIP5K3 and PIP4K2C 1.Expression of divalent metal-ion transporter 1 (DMT1) in HCT116 is demonstrated to be markedly decreasedunder stimulation with TNF for 24 and 48 h, while JNK-IN-7 can significantly reverse the decrease. TNF candown-regulate DMT1 expression, while JNK-IN-7 can markedly supp

5、ress this function 2.PROTOCOLCell Assay 2 Intestinal epithelial cell line (HCT116) is cultured in DMEM medium. To determine the mechanisms of TNFinvolved in regulating DMT1 expression, JNK-IN-7 (1 M), NF-B inhibitor (BAY 11-7082, 1 M), andcaspase-3/8 inhibitor (Z-DEVD-FMK, 50 M) are also added into

6、the culture medium. After 48 h of culture,cells are then collected to detect the expression of DMT1 by qRT-PCR 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) J Allergy Clin Immunol. 2019 Jul 18. pii: S0091-6749(19)30832-2.See more custome

7、r validations on HYPERLINK / www.MedChemEREFERENCES1. Zhang T, et al. Discovery of Potent and Selective Covalent Inhibitors of JNK. Chem Biol. 2012 Jan 27;19(1):140-54.2. Wu W, et al. Divalent metal-ion transporter 1 is decreased in intestinal epithelial cells and contributes to the anemia in inflammatorybowel disease. Sci Rep. 2015 Nov 17;5:16344.McePdfHeightCaution: Product has not been fully validated for medical applications.For researc