1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEAlkyne tyramideCat. No.: HY-131442CAS No.: 1694495-59-4分式: CHNO分量: 217.26作靶點: Others作通路: Others儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 100 mg/mL (460.28 mM; Need ultrasonic)Mass Sol

2、vent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 4.6028 mL 23.0139 mL 46.0278 mL5 mM 0.9206 mL 4.6028 mL 9.2056 mL10 mM 0.4603 mL 2.3014 mL 4.6028 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。體內(nèi)實驗 請根據(jù)您的實驗動物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前請先配制澄的儲備液，再依次添加助溶?為保證實驗結(jié)果的可靠性，體內(nèi)實驗的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；以下溶劑前的百分指該溶劑在您

3、配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (11.51 mM); Clear solution2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (11.51 mM); Clear solution3. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (11.51 mM); Clear solution1/2

4、Master of Small Molecules 您邊的抑制劑師www.MedChemEBIOLOGICAL ACTIVITY物活性 Alkyne tyramide種抗壞酸過氧化物酶 2 (APEX2) 探針。Alkyne tyramide 顯著提了完整酵母細胞的APEX 標(biāo)記效率， APEX2 底物物素苯酚 (BP) 具有更的細胞壁滲透性。Alkyne tyramide 還有助于鑒定APEX 標(biāo)記位點，明確線粒體蛋的膜拓?fù)涞姆峙洹ｓw外研究 Yeast cells expressing Su9-APEX2 are incubated with each probe at 2.5 mM fin

5、al concentration for half anhour, and the labeling reaction is initiated by the addition of 1 mM H2O2. Alkyne tyramide is the most reactiveAPEX2 substrate for labeling the yeast proteome 1.APEX2-mediated Alkyne tyramide labeling in the yeast mitochondria is more effective at probeconcentrations abov

6、e 1 mM 1.Alkyne tyramide enables proteomic profiling in the mitochondrial matrix of intact yeast cells with exceptionallyhigh specificity (94%), and offers higher coverage than the traditional APEX2 substrate biotin-phenol (BP) 1.REFERENCES1. Li Y, et al. A Clickable APEX Probe for Proximity-Dependent Proteomic Profiling in Yeast. Cell Chem Biol. 2020;27(7):858-865.e8.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use only.Tel