牛結(jié)核病防治技術(shù)規(guī)范牛結(jié)核病防治技術(shù)規(guī)范牛結(jié)核病防治技術(shù)規(guī)范牛結(jié)核病防治技術(shù)規(guī)范編制僅供參考審核批準(zhǔn)生效日期地址：電話：傳真:郵編:六、牛結(jié)核病防治技術(shù)規(guī)范牛結(jié)核?。˙ovineTuberculosis）是由牛型結(jié)核分枝桿菌（Mycobacteriumbovis）引起的一種人獸共患的慢性傳染病，我國(guó)將其列為二類動(dòng)物疫病。為了預(yù)防、控制和凈化牛結(jié)核病，根據(jù)《中華人民共和國(guó)動(dòng)物防疫法》及有關(guān)的法律法規(guī)，特制定本規(guī)范。1適用范圍本規(guī)范規(guī)定了牛結(jié)核病的診斷、疫情報(bào)告、疫情處理、防治措施、控制和凈化標(biāo)準(zhǔn)。本規(guī)范適用于中華人民共和國(guó)境內(nèi)從事飼養(yǎng)、生產(chǎn)、經(jīng)營(yíng)牛及其產(chǎn)品，以及從事相關(guān)動(dòng)物防疫活動(dòng)的單位和個(gè)人。2診斷流行特點(diǎn)本病奶牛最易感，其次為水牛、黃牛、牦牛。人也可被感染。結(jié)核病病牛是本病的主要傳染源。牛型結(jié)核分枝桿菌隨鼻汁、痰液、糞便和乳汁等排出體外，健康?？赏ㄟ^(guò)被污染的空氣、飼料、飲水等經(jīng)呼吸道、消化道等途徑感染。臨床特征：潛伏期一般為3～6周，有的可長(zhǎng)達(dá)數(shù)月或數(shù)年。臨床通常呈慢性經(jīng)過(guò)，以肺結(jié)核、乳房結(jié)核和腸結(jié)核最為常見(jiàn)。肺結(jié)核：以長(zhǎng)期頑固性干咳為特征，且以清晨最為明顯?；夹笕菀灼冢饾u消瘦，病情嚴(yán)重者可見(jiàn)呼吸困難。乳房結(jié)核：一般先是乳房淋巴結(jié)腫大，繼而后方乳腺區(qū)發(fā)生局限性或彌漫性硬結(jié)，硬結(jié)無(wú)熱無(wú)痛，表面凹凸不平。泌乳量下降，乳汁變稀，嚴(yán)重時(shí)乳腺萎縮，泌乳停止。腸結(jié)核：消瘦,持續(xù)下痢與便秘交替出現(xiàn)，糞便常帶血或膿汁。病理變化：在肺臟、乳房和胃腸粘膜等處形成特異性白色或黃白色結(jié)節(jié)。結(jié)節(jié)大小不一，切面干酪樣壞死或鈣化，有時(shí)壞死組織溶解和軟化，排出后形成空洞。胸膜和肺膜可發(fā)生密集的結(jié)核結(jié)節(jié)，形如珍珠狀。實(shí)驗(yàn)室診斷病原學(xué)診斷采集病牛的病灶、痰、尿、糞便、乳及其它分泌物樣品，作抹片或集菌處理（見(jiàn)附件）后抹片，用抗酸染色法染色鏡檢，并進(jìn)行病原分離培養(yǎng)和動(dòng)物接種等試驗(yàn)。免疫學(xué)試驗(yàn)牛型結(jié)核分枝桿菌PPD（提純蛋白衍生物）皮內(nèi)變態(tài)反應(yīng)試驗(yàn)（即牛提純結(jié)核菌素皮內(nèi)變態(tài)反應(yīng)試驗(yàn)）（見(jiàn)GB/T18646）。結(jié)果判定本病依據(jù)流行病學(xué)特點(diǎn)、臨床特征、病理變化可做出初步診斷。確診需進(jìn)一步做病原學(xué)診斷或免疫學(xué)診斷。分離出結(jié)核分枝桿菌（包括牛結(jié)核分枝桿菌、結(jié)核分枝桿菌）判為結(jié)核病牛。牛型結(jié)核分枝桿菌PPD皮內(nèi)變態(tài)反應(yīng)試驗(yàn)陽(yáng)性的牛，判為結(jié)核病牛。3疫情報(bào)告任何單位和個(gè)人發(fā)現(xiàn)疑似病牛，應(yīng)當(dāng)及時(shí)向當(dāng)?shù)貏?dòng)物防疫監(jiān)督機(jī)構(gòu)報(bào)告。動(dòng)物防疫監(jiān)督機(jī)構(gòu)接到疫情報(bào)告并確認(rèn)后，按《動(dòng)物疫情報(bào)告管理辦法》及有關(guān)規(guī)定及時(shí)上報(bào)。4疫情處理發(fā)現(xiàn)疑似疫情，畜主應(yīng)限制動(dòng)物移動(dòng)；對(duì)疑似患病動(dòng)物應(yīng)立即隔離。動(dòng)物防疫監(jiān)督機(jī)構(gòu)要及時(shí)派員到現(xiàn)場(chǎng)進(jìn)行調(diào)查核實(shí)，開(kāi)展實(shí)驗(yàn)室診斷。確診后，當(dāng)?shù)厝嗣裾M織有關(guān)部門按下列要求處理：撲殺對(duì)患病動(dòng)物全部撲殺。隔離對(duì)受威脅的畜群（病畜的同群畜）實(shí)施隔離，可采用圈養(yǎng)和固定草場(chǎng)放牧兩種方式隔離。隔離飼養(yǎng)用草場(chǎng)，不要靠近交通要道，居民點(diǎn)或人畜密集的地區(qū)。場(chǎng)地周圍最好有自然屏障或人工柵欄。對(duì)隔離畜群的結(jié)核病凈化，按本規(guī)范規(guī)定進(jìn)行。無(wú)害化處理病死和撲殺的病畜，要按照GB16548-1996《畜禽病害肉尸及其產(chǎn)品無(wú)害化處理規(guī)程》進(jìn)行無(wú)害化處理。流行病學(xué)調(diào)查及檢測(cè)開(kāi)展流行病學(xué)調(diào)查和疫源追蹤；對(duì)同群動(dòng)物進(jìn)行檢測(cè)。消毒對(duì)病畜和陽(yáng)性畜污染的場(chǎng)所、用具、物品進(jìn)行嚴(yán)格消毒。飼養(yǎng)場(chǎng)的金屬設(shè)施、設(shè)備可采取火焰、熏蒸等方式消毒；養(yǎng)畜場(chǎng)的圈舍、場(chǎng)地、車輛等，可選用2%燒堿等有效消毒藥消毒；飼養(yǎng)場(chǎng)的飼料、墊料可采取深埋發(fā)酵處理或焚燒處理；糞便采取堆積密封發(fā)酵方式，以及其它相應(yīng)的有效消毒方式。發(fā)生重大牛結(jié)核病疫情時(shí)，當(dāng)?shù)乜h級(jí)以上人民政府應(yīng)按照《重大動(dòng)物疫情應(yīng)急條例》有關(guān)規(guī)定，采取相應(yīng)的疫情撲滅措施。5預(yù)防與控制采取以“監(jiān)測(cè)、檢疫、撲殺和消毒”相結(jié)合的綜合性防治措施。監(jiān)測(cè)監(jiān)測(cè)對(duì)象：牛監(jiān)測(cè)比例為：種牛、奶牛100%，規(guī)模場(chǎng)肉牛10%，其它牛5%，疑似病牛100%。如在牛結(jié)核病凈化群中（包括犢牛群）檢出陽(yáng)性牛時(shí)，應(yīng)及時(shí)撲殺陽(yáng)性牛，其它牛按假定健康群處理。成年牛凈化群每年春秋兩季用牛型結(jié)核分枝桿菌PPD皮內(nèi)變態(tài)反應(yīng)試驗(yàn)各進(jìn)行一次監(jiān)測(cè)。初生犢牛，應(yīng)于20日齡時(shí)進(jìn)行第一次監(jiān)測(cè)。并按規(guī)定使用和填寫監(jiān)測(cè)結(jié)果報(bào)告，及時(shí)上報(bào)。檢疫異地調(diào)運(yùn)的動(dòng)物，必須來(lái)自于非疫區(qū)，憑當(dāng)?shù)貏?dòng)物防疫監(jiān)督機(jī)構(gòu)出具的檢疫合格證明調(diào)運(yùn)。動(dòng)物防疫監(jiān)督機(jī)構(gòu)應(yīng)對(duì)調(diào)運(yùn)的種用、乳用、役用動(dòng)物進(jìn)行實(shí)驗(yàn)室檢測(cè)。檢測(cè)合格后，方可出具檢疫合格證明。調(diào)入后應(yīng)隔離飼養(yǎng)30天，經(jīng)當(dāng)?shù)貏?dòng)物防疫監(jiān)督機(jī)構(gòu)檢疫合格后，方可解除隔離。人員防護(hù)飼養(yǎng)人員每年要定期進(jìn)行健康檢查。發(fā)現(xiàn)患有結(jié)核病的應(yīng)調(diào)離崗位，及時(shí)治療。防疫監(jiān)督結(jié)核病監(jiān)測(cè)合格應(yīng)為奶牛場(chǎng)、種畜場(chǎng)《動(dòng)物防疫合格證》發(fā)放或?qū)忩?yàn)的必備條件。動(dòng)物防疫監(jiān)督機(jī)構(gòu)要對(duì)轄區(qū)內(nèi)奶牛場(chǎng)、種畜場(chǎng)的檢疫凈化情況監(jiān)督檢查。鮮奶收購(gòu)點(diǎn)（站）必須憑奶牛健康證明收購(gòu)鮮奶。凈化措施被確診為結(jié)核病牛的牛群（場(chǎng)）為牛結(jié)核病污染群（場(chǎng)），應(yīng)全部實(shí)施牛結(jié)核病凈化。牛結(jié)核病凈化群（場(chǎng)）的建立污染牛群的處理：應(yīng)用牛型結(jié)核分枝桿菌PPD皮內(nèi)變態(tài)反應(yīng)試驗(yàn)對(duì)該牛群進(jìn)行反復(fù)監(jiān)測(cè)，每次間隔3個(gè)月，發(fā)現(xiàn)陽(yáng)性牛及時(shí)撲殺，并按照本規(guī)范4規(guī)定處理。犢牛應(yīng)于20日齡時(shí)進(jìn)行第一次監(jiān)測(cè)，100～120日齡時(shí)，進(jìn)行第二次監(jiān)測(cè)。凡連續(xù)兩次以上監(jiān)測(cè)結(jié)果均為陰性者，可認(rèn)為是牛結(jié)核病凈化群。凡牛型結(jié)核分枝桿菌PPD皮內(nèi)變態(tài)反應(yīng)試驗(yàn)疑似反應(yīng)者，于42天后進(jìn)行復(fù)檢，復(fù)檢結(jié)果為陽(yáng)性，則按陽(yáng)性牛處理;若仍呈疑似反應(yīng)則間隔42天再?gòu)?fù)檢一次，結(jié)果仍為可疑反應(yīng)者，視同陽(yáng)性牛處理。隔離疑似結(jié)核病?；蚺Ｐ徒Y(jié)核分枝桿菌PPD皮內(nèi)變態(tài)反應(yīng)試驗(yàn)可疑畜須隔離復(fù)檢。消毒臨時(shí)消毒：奶牛群中檢出并剔出結(jié)核病牛后，牛舍、用具及運(yùn)動(dòng)場(chǎng)所等按照規(guī)定進(jìn)行緊急處理。經(jīng)常性消毒：飼養(yǎng)場(chǎng)及牛舍出入口處，應(yīng)設(shè)置消毒池，內(nèi)置有效消毒劑，如3～5%來(lái)蘇爾溶液或20％石灰乳等。消毒藥要定期更換，以保證一定的藥效。牛舍內(nèi)的一切用具應(yīng)定期消毒;產(chǎn)房每周進(jìn)行一次大消毒，分娩室在臨產(chǎn)牛生產(chǎn)前及分娩后各進(jìn)行一次消毒。

附件樣品集菌方法痰液或乳汁等樣品，由于含菌量較少，如直接涂片鏡檢往往是陰性結(jié)果。此外，在培養(yǎng)或作動(dòng)物試驗(yàn)時(shí)，常因污染雜菌生長(zhǎng)較快，使病原結(jié)核分枝桿菌被抑制。下列幾種消化濃縮方法可使檢驗(yàn)標(biāo)本中蛋白質(zhì)溶解、殺滅污染雜菌，而結(jié)核分枝桿菌因有蠟質(zhì)外膜而不死亡，并得到濃縮。1硫酸消化法用4－6％硫酸溶液將痰、尿、糞或病灶組織等按1:5之比例加入混合，然后置37℃作用1－2小時(shí)，經(jīng)3000－4000rpm離心30分鐘，棄上清，取沉淀物涂片鏡檢、培養(yǎng)和接種動(dòng)物。也可用硫酸消化濃縮后，在沉淀物中加入3％氫氧化鈉中和，然后抹片鏡檢、培養(yǎng)和接種動(dòng)物。2氫氧化鈉消化法取氫氧化鈉35－40g，鉀明礬2g，溴麝香草酚蘭20mg（預(yù)先用60％酒精配制成％濃度，應(yīng)用時(shí)按比例加入），蒸餾水1000mL混合，即為氫氧化鈉消化液。將被檢的痰、尿、糞便或病灶組織按1:5的比例加入氫氧化鈉消化液中，混勻后，37℃作用2－3小時(shí)，然后無(wú)菌滴加5－10％鹽酸溶液進(jìn)行中和，使標(biāo)本的pH調(diào)到左右（此時(shí)顯淡黃綠色），以3000－4000rpm離心15－20分鐘，棄上清，取沉淀物涂片鏡檢、培養(yǎng)和接種動(dòng)物。在病料中加入等量的4％氫氧化鈉溶液，充分振搖5－10分鐘，然后用3000rpm離心15－20分鐘，棄上清，加1滴酚紅指示劑于沉淀物中，用2N鹽酸中和至淡紅色，然后取沉淀物涂片鏡檢、培養(yǎng)和接種動(dòng)物。在痰液或小膿塊中加入等量的1％氫氧化鈉溶液，充分振搖15分鐘，然后用3000rpm離心30分鐘，取沉淀物涂片鏡檢、培養(yǎng)和接種動(dòng)物。對(duì)痰液的消化濃縮也可采用以下較溫和的處理方法：取1N（或4％）氫氧化鈉水溶液50mL，L檸檬酸鈉50mL，N－乙酰－L－半胱氨酸，混合。取痰一份，加上述溶液2份，作用24－48小時(shí)，以3000rpm離心15分鐘，取沉淀物涂片鏡檢、培養(yǎng)和接種動(dòng)物。3安替福民（Antiformin）沉淀濃縮法溶液A：碳酸鈉12g、漂白粉8g、蒸餾水80mL。溶液B：氫氧化鈉15g、蒸餾水85mL。應(yīng)用時(shí)A、B兩液等量混合，再用蒸餾水稀釋成15－20％后使用，該溶液須存放于棕色瓶?jī)?nèi)。將被檢樣品置于試管中，加入3－4倍量的15－20％安替福民溶液，充分搖勻后37℃作用1小時(shí)，加1－2倍量的滅菌蒸餾水，搖勻，3000－4000rpm離心20－30分鐘，棄上清沉淀物加蒸餾水恢復(fù)原量后再離心一次，取沉淀物涂片鏡檢、培養(yǎng)和接種動(dòng)物。PrescribedandalternativediagnostictestsforOIElisteddiseasesTerrestrialCodeChapterNo.TerrestrialManualChapterNo.DiseasenamePrescribedtestsAlternativetests.tuberculosisTuberculintestGammainterferontestCountryorzonefreefrombovinetuberculosisToqualifyasfreefrombovinetuberculosis,acountryorzoneshouldsatisfythefollowingrequirements:M.bovisinfectionindomestic(permanentlycaptiveandownedfree-range)bovinesincludingcattle,waterbuffaloandwoodbisonisanotifiablediseaseinthecountry;anon-goingawarenessprogrammeshouldbeinplacetoencouragereportingofallcasessuggestiveofbovinetuberculosis;regularandperiodictestingofallcattle,waterbuffalo,andwoodbisonherdsdemonstratedthatM.

bovisinfectionwasnotpresentinatleast%oftheherdsand%ofthecattle,waterbuffaloandwoodbisoninthecountryorzonefor3

consecutiveyears;asurveillanceprogrammeshouldbeinplacetodetectbovinetuberculosisinthecountryorzonethroughante-mortemandpost-morteminspectionasdescribedinChapter

.;ifthesurveillanceprogrammedescribedinpoints

3and4abovehasnotdetectedinfectionwithM.

bovisfor5

consecutiveyears,surveillancemaybemaintainedthroughante-mortemandpost-morteminspectionasdescribedinChapter.;cattle,waterbuffaloandwoodbisonintroducedintoacountryorzonefreefrombovinetuberculosisshouldbeaccompaniedbyacertificatefromanOfficialVeterinarianattestingthattheycomefromacountry,zone,compartmentorherdfreefrombovinetuberculosisorcomplywiththerelevantprovisionsinArticle

orinArticle

.bovinetuberculosisArticleprovisionsTherecommendationsinthischapterareintendedtomanagethehumanandanimalhealthrisksassociatedwithMycobacteriumbovis(M.

bovis)infectionindomestic(permanentlycaptiveandownedfree-range)bovinesincludingcattle(Bostaurus,B.

indicusandB.

grunniens),waterbuffaloes(Bubalusbubalis)andwoodbisons(BisonbisonandB.bonasus).StandardsfordiagnostictestsaredescribedintheTerrestrialManual.ArticleorzonefreefrombovinetuberculosisToqualifyasfreefrombovinetuberculosis,acountryorzoneshouldsatisfythefollowingrequirements:M.bovisinfectionindomestic(permanentlycaptiveandownedfree-range)bovinesincludingcattle,waterbuffaloandwoodbisonisanotifiablediseaseinthecountry;anon-goingawarenessprogrammeshouldbeinplacetoencouragereportingofallcasessuggestiveofbovinetuberculosis;regularandperiodictestingofallcattle,waterbuffalo,andwoodbisonherdsdemonstratedthatM.

bovisinfectionwasnotpresentinatleast%oftheherdsand%ofthecattle,waterbuffaloandwoodbisoninthecountryorzonefor3

consecutiveyears;asurveillanceprogrammeshouldbeinplacetodetectbovinetuberculosisinthecountryorzonethroughante-mortemandpost-morteminspectionasdescribedinChapter

.;ifthesurveillanceprogrammedescribedinpoints

3and4abovehasnotdetectedinfectionwithM.

bovisfor5

consecutiveyears,surveillancemaybemaintainedthroughante-mortemandpost-morteminspectionasdescribedinChapter.;cattle,waterbuffaloandwoodbisonintroducedintoacountryorzonefreefrombovinetuberculosisshouldbeaccompaniedbyacertificatefromanOfficialVeterinarianattestingthattheycomefromacountry,zone,compartmentorherdfreefrombovinetuberculosisorcomplywiththerelevantprovisionsinArticle

orinArticle

freefrombovinetuberculosisToqualifyasacompartmentfreefrombovinetuberculosis,cattle,waterbuffaloorwoodbisoninacompartmentshouldbecertifiedbytheVeterinaryAuthorityassatisfyingthefollowingrequirements:cattle,waterbuffaloandwoodbison:showednosignofbovinetuberculosisorlesionsatante-mortemorpost-morteminspectionforatleast3

consecutiveyears;wereover6

weeksofageatthetimeofthefirsttestandhaveshownanegativeresulttoatleasttwotuberculintestscarriedoutatanintervalofaminimumof6

months,thefirsttestbeingperformedatleast6

monthsfollowingtheslaughterofthelastaffectedanimal;metoneofthefollowingconditions:showedanegativeresulttoabiannualtuberculintesttoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisismorethan1%ofallherdsinthecountryorzoneduringthelast2

years;orshowedanegativeresulttoanannualtuberculintesttoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisismorethan%butnotmorethan1%ofallherdsinthecountryorzoneduringthelast2

years;orshowedanegativeresulttoatuberculintestevery3

yearstoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisisnotmorethan%ofallherdsinthecountryorzoneduringthelast4

years;orshowedanegativeresulttoatuberculintestevery4

yearstoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisisnotmorethan%ofallherdsinthecountryorzoneduringthelast6years;cattle,waterbuffaloandwoodbisonintroducedintothecompartmentcomefromaherdfreefrombovinetuberculosis.Thisconditionmaybewaivedforanimalswhichhavebeenisolatedforatleast90

daysandwhich,priortoentryintothecompartment,weresubjectedtoatleasttwotuberculintestscarriedoutata6-monthintervalwithnegativeresultswiththesecondtuberculintestperformedduringthe30dayspriortoentryintothecompartment;cattle,waterbuffaloandwoodbisoninacompartmentfreefrombovinetuberculosisareprotectedfromcontactwithwildlifereservoirsofbovinetuberculosisandaremanagedunderacommonbiosecurityplanprotectingthemfromcontaminationwithM.

bovis,andthecompartmenthasbeenapprovedbytheVeterinaryAuthorityinaccordancewithChapters

.and.ArticlefreefrombovinetuberculosisToqualifyasfreefrombovinetuberculosis,aherdofcattle,waterbuffalo,orwoodbisonsshouldsatisfythefollowingrequirements:theherdisinacountry,zoneorcompartmentfreefrombovinetuberculosisandiscertifiedfreebytheVeterinaryAuthority;orcattle,waterbuffaloandwoodbisonintheherd:showednosignsofbovinetuberculosisorlesionsatante-mortemorpost-morteminspectionforatleast3

consecutiveyears;wereover6

weeksofageatthetimeofthefirsttestandhaveshownanegativeresulttoatleasttwotuberculintestscarriedoutatanintervalofaminimumof6months,thefirsttestbeingperformedatleast6monthsfollowingtheslaughterofthelastaffectedanimal;metoneofthefollowingconditions:showedanegativeresulttoanannualtuberculintesttoensurethecontinuingabsenceofbovinetuberculosis;orshowedanegativeresulttoatuberculintestevery2yearstoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisisnotmorethan1%ofallherdsinthecountryorzoneduringthelast2years;orshowedanegativeresulttoatuberculintestevery3yearstoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisisnotmorethan%ofallherdsinthecountryorzoneduringthelast4years;orshowedanegativeresulttoatuberculintestevery4yearstoensurethecontinuingabsenceofbovinetuberculosisiftheannualpercentageofherdsconfirmedasinfectedwithtuberculosisisnotmorethan%ofallherdsinthecountryorzoneduringthelast6years;cattle,waterbuffaloandwoodbisonintroducedintotheherdcomefromaherdfreefrombovinetuberculosis.Thisconditionmaybewaivedforanimalswhichhavebeenisolatedforatleast90daysandwhich,priortoentryintotheherd,weresubjectedtoatleasttwotuberculintestscarriedoutata6-monthintervalwithnegativeresults.Articlefortheimportationofcattle,waterbuffaloandwoodbisonforbreedingorrearingVeterinaryAuthoritiesofimportingcountriesshouldrequirethepresentationofaninternationalveterinarycertificateattestingthattheanimals:showednosignsofbovinetuberculosisonthedayofshipment;originatefromaherdfreefrombovinetuberculosisthatisinacountry,zoneorcompartmentfreefrombovinetuberculosis;orweresubjectedtothetuberculintestforbovinetuberculosiswithnegativeresultsduringthe30dayspriortoshipmentandcomefromaherdfreefrombovinetuberculosis;orhavebeenisolatedforatleast90dayspriortoentryintotheherdincludingprotectionfromcontactwithwildlifereservoirsofbovinetuberculosisandweresubjectedtoatleasttwotuberculintestscarriedoutatasix-monthintervalwithnegativeresultswiththesecondtuberculintestperformedduringthe30dayspriortoentryintotheherd.Articlefortheimportationofcattle,waterbuffaloandwoodbisonforslaughterVeterinaryAuthoritiesofimportingcountriesshouldrequirethepresentationofaninternationalveterinarycertificateattestingthattheanimals:showednosignsofbovinetuberculosisonthedayofshipment;originatedfromaherdfreefrombovinetuberculosisorweresubjectedtoatuberculintestforbovinetuberculosiswithnegativeresultsduringthe30dayspriortoshipment;werenotbeingeliminatedaspartofaneradicationprogrammeagainstbovinetuberculosis.Articlefortheimportationofsemenofcattle,waterbuffaloandwoodbisonVeterinaryAuthoritiesofimportingcountriesshouldrequirethepresentationofaninternationalveterinarycertificateattestingthat:thedonoranimalsshowednosignsofbovinetuberculosisonthedayofcollectionofthesemenandeither:werekeptinanartificialinseminationcentrefreefrombovinetuberculosisinacountry,zoneorcompartmentfreefrombovinetuberculosisandwhichonlyacceptsanimalsfromfreeherdsinafreecountry,zoneorcompartment;orshowednegativeresultstotuberculintestscarriedoutannuallyandwerekeptinaherdfreefrombovinetuberculosis;thesemenwascollected,processedandstoredinconformitywiththeprovisionsofChapter

.andChapter

.Articlefortheimportationofembryos/ovaofcattle,waterbuffaloandwoodbisonVeterinaryAuthoritiesofimportingcountriesshouldrequirethepresentationofaninternationalveterinarycertificateattestingthat:thedonorfemalesandallothersusceptibleanimalsintheherdoforiginshowednosignsofbovine