Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEErythrosineBCat.No.:HY-D0259CASNo.:16423-68-0Synonyms:Erythrosinextrabluish分?式:C??H?I?Na?O?分?量:879.86作?靶點(diǎn):Others作?通路:Others儲存?式:4°C,sealedstorage,awayfrommoistureandlight*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoistureandlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn)H2O:33.33mg/mL(37.88mM;Needultrasonic)DMSO:33.33mg/mL(37.88mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM1.1365mL5.6827mL11.3654mL5mM0.2273mL1.1365mL2.2731mL10mM0.1137mL0.5683mL1.1365mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoistureandlight)。-80°C儲存時，請?jiān)?個?內(nèi)使?，-20°C儲存時，請?jiān)?個?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%saline1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESolubility:≥2.5mg/mL(2.84mM);Clearsolution2.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(2.84mM);ClearsolutionBIOLOGICALACTIVITY?物活性ErythrosineB?泛?于?品和紡織?業(yè)的?造染料。ErythrosineB也?種新型的光敏劑，已被?于開發(fā)動物模型。體外研究OnlythetwohighestconcentrationsofErythrosineBtested(50.0and70.0μg/mL)aresignificantlydifferent(p[1].PROTOCOLCellAssay[1]Thealkalinesingle-cellgelelectrophoresisassay(cometassay)isperformed.Briefly,2×105HepG2cellsareseededina24-wellplatefor24h.ThecellsarethentreatedwithErythrosineBat0.1,0.2,2.0,10.0,25.0,50.0or70.0μg/mL(finalconcentration)for4h;vehiclecontrol(0.7%DMSO)andpositivecontrol(doxorubicin0.3μg/mL)treatmentsarealsoperformed.TheHepG2cellsuspensionismixedwith37°Clow-meltingpointagaroseandtransferredtonormal-meltingpointagarose-coatedslides.Onehundredrandomlychosennucleoidsareanalyzedpertreatment,andatotalofthreeindependentexperimentsareperformed[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.REFERENCES[1].ChenW,etal.Establishinganexperimentalratmodelofphotodynamically-inducedretinalveinocclusionusingerythrosinB.IntJOphthalmol.2014Apr18;7(2):232-8.[2].ChequerFM,etal.GenotoxicandmutageniceffectsoferythrosineB,axanthenefooddye,onHepG2cells.FoodChemToxicol.2012Oct;50(10):3447-51.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.