微流體芯片資料第1頁/共31頁ContentsBackgroundExperimentABSTRACTDiscussion第2頁/共31頁ABSTRACTMicrodropletso?eruniquecompartmentsforaccommodatingalargenumberofchemicalandbiologicalreactionsintinyvolumewithprecisecontrol.Amajorconcernindroplet-basedmicro?uidicsisthedi?cultytoaddressdropletsindividuallyandachievehighthroughputatthesametime.第3頁/共31頁Here,wehavecombinedanimprovedcartridgesamplingtechniquewithamicro?uidicchiptoperformdropletscreeningsandaggressivereactionwithreagentconsumption.Ourcombineddeviceprovidesasimplemodeltoutilizemultipledropletsforvariousreactionswithlowreagentconsumptionandhighthroughput.第4頁/共31頁第5頁/共31頁BackgroundAlargenumberofreactionscanbeindependentlyminiaturizedintotinydropletsrangingfromnanolitertopicolitersizeswithouthavingtoincreasethechipsizeandcomplexity.

Microfluidicchipshaveprovidedapowerfulplatformforaccomplishingdropletformationandmanipulationwithinonechipofonlyafewsquarecentimetersinsize.

AdvantagesOverthepastfewyears,droplet-basedmicro?uidicshasattractedincreasingattention.第6頁/共31頁Background

Dropletgenerationformultiplesamplesislimitedbythe“world-to-chip”interfaceproblemandcannotbeaddressedindividuallyDropletgenerationandmanipulationfunctionswithinonechipcaneasilyinterferewitheachotherandaredifficultTobalance,despitebeingseparatedbyalong,windingchannel.DrawbackPassivedropletformationmethods:第7頁/共31頁InnovateThiscontextshowsahybriddevice:thecartridgedropletgenerationtechniquecombinedwithamicrofluidicchip.Theyimprovedthecartridgetechniquewithanovelmovementscheme:thecapillary,insteadofthemultiwellplate,wasmovedbyadigitalandautomaticmanipulatortoaspiratedifferentsamplesorthecoveringoil.第8頁/共31頁Thehybriddropletdeviceforgenerationandmanipulationconsistsoffourmajorcomponents.

(a)manipulator(b)multiwellplate(c)capillary(d)PDMSchip第9頁/共31頁第10頁/共31頁第11頁/共31頁Threegeneralsteps

loadthemultiwellplatewithdifferentsamples,coverwithalayerofoil,andinputtheparametersofthedesireddropletarrayintothecontrollerviaaPC

startthecontrollertoaspiratecertainvolumesofsamplesandoilintotheCapillarysequentially

introducethegenerateddropletsintothePDMSchipformerging,mixing,detection,orothermanipulation.第12頁/共31頁Laboratoryreagent

siliconeoil,

FeCl2·4H2O(Sigma?Aldrich),FeCl3·6H2O(AcrosOrganics),ammoniumhydroxidesolution(28%NH3Fluka),andhydrochloricacid(37%HCl,AcrosOrganics)第13頁/共31頁ImprovedSamplingTechnique

CombinedDropletDevicePerformance

High-ThroughputDropletScreening

AggressiveDropletReaction

第14頁/共31頁ImprovedSamplingTechniqueThecapillaryaspiratestheoilwhenthecantileverremainsinthe“up”state.Whenthecantileveristriggeredtostayinthe“down”state,thecapillaryaspiratesthesample.Theresidencetimeintheoil(TO)andsample(TS)couldbeadjustedrespectivelytogeneratedropletswithdesireddistanceandvolume.第15頁/共31頁第16頁/共31頁(c)Relativeintensitywasmeasuredalongthechannelstartingfromtoprightcorner.TheintensityoffluoresceinandDIwaterdropletdoesnotdecreaseorincrease,whichindicatesthatnocross-contaminationbetweenthesetwosampleswasdetected.(b)Dropletarraywasgeneratedbysequentiallyaspirating50uMfluoresceinandDIwater.第17頁/共31頁CombinedDropletDevicePerformanceHere,themergingofdropletswasdemonstratedinaPDMSchip.ThedropletsfusiondoesnotdependondropletdistancebutdropletvelocityandVd/Vc.Aftermixingcompletely,themergeddropletwasoutputtoasinuouschannelfordisplayanddetection.第18頁/共31頁第19頁/共31頁Inthe?rstapplication,aseriesofAiandBm,n(i=1?2,m=1?3,andn=1?6)weregenerated.A1andA2areFluo-4andRhod-2solutionswiththesameconcentration(10.8μM),whichareindicatorsthatexhibitanincreasein?uorescence(green/red)uponmetalionbonding.Avalueofm=1?3representmetalsionsolutionsofLaIII,YbIII,andYIII,respectively,whilen=1?6aresixdi?erentmetalionconcentrations(0,2.5,5,10,20,and40μM)ofthesesolutions.Thedropletarraycontains721-nLdroplets第20頁/共31頁High-ThroughputDropletScreening第21頁/共31頁第22頁/共31頁The36pairsofdropletreactionsused～72nLofsolutionandrequired1.8mintocomplete.Therefore,thereagentconsumptionforeachmeasurementcouldbereducedto1nLpersample,andthereactionthroughputis1200h?1,whichcanbemuchfasterbyincreasingthe?owvelocity.ThescreeningdropletscouldbestoredinthePDMSchipafterwestoppedthevacuum.第23頁/共31頁AggressiveDropletReaction第24頁/共31頁Fromtheseimages,wefoundthatthecoprecipitationissofastthatitimmediatelyformsparticleswhentwodropletscomeintocontactwitheachother.Moreover,ineachvolumeratio,theprecipitationinitiatedbythefusionoftwodropletsdidnotblockthemicrochannelatsuchhighconcentrations.Such?exible,controllableandminiaturizedreactionconditionsareextremelysuitableforaggressive,fast,orevenexplosivereactionsthatgenerateprecipitationorheat.第25頁/共31頁DiscussionThecartridgedropletgenerationmethodprovidesanefficientwayforintroducingdifferentnanoliterdropletsintothemicrofluidicchip.themicrofluidicchipoffersaplatformfordelicatemanipulationsuchasmerging.Thisfeatureisvaluableforreducingthereactionscreeningandoptimizationcostandtimeandisparticularlyusefulfordrugscreening.第26頁/共31頁Comparedwiththeothercartridgedropletgeneration,ourdesignusedadigitalandautomaticmanipulatortomovethecapillaryinsteadofthemultiwellplate.DiscussionSubsequently,thecoveringoilwasingeniouslyuseda